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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 106 for “"cytochrome b"”.
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Molecular evolution of the cytochrome b gene among percid fishes
To better understand the evolution of the cytochrome b gene and its implications for evolutionary relationships among percid fishes, the entire cytochrome b gene (1,140 base pairs) was amplified via PCR using the tRNA flanking primers and sequenced. Twenty-two species were examined, including 20 …
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Cloning, High-Level Expression, and Characterization of Escherichia Coli Cytochrome B(562)
The gene for the soluble cytochrome b$\sb{562}$ from Escherichia coli B was cloned on a SalI fragment containing the full length gene, including the promotor. The DNA sequence analysis of the gene revealed the presence of a leader sequence, with the characteristics of an E. coli signal sequence, in …
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The role of electrostatics in macromolecular associations: The cytochrome b(5)-cytochrome c complex
… interactions, including the association between cytochrome b$\sb5$ and cytochrome c. The dependence of stability of the association between cytochrome b$\sb5$ and cytochrome c on ionic strength and pH conditions is indicative of the charge-charge interactions in the interfacial domain.
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Investigation of the Interaction Between Cytochrome b(H) and the Q(i)-Site in Rhodobacter Sphaeroides
… the pH dependence of the midpoint potential of cytochrome bH differs substantially from what was published previously; a high potential component of bH remains even in the presence of Antimycin, and the thermodynamic properties of bH are substantially modified in strains with mutations at …
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The role of the cytochrome B and cytochrome C oxidase III genes in the immune response of the South African abalone, Haliotis midae
… Two genes of the electron transport system, cytochrome b and cytochrome c oxidase III, were found to be upregulated in a cDNA microarray experiment performed on haemocytes from immunestimulated abalone (Arendze-Bailey, unpublished). The current study sought to confirm these results by …
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Use of Fluorescence to Study the Membrane Binding Domain of Cytochrome B(5) and the Induction of Membrane Domain Formation in Phospholipid Vesicles by Myelin Basic Protein
Embargo set by: Seth Robbins for item 86158 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs
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Comparison of Cytochrome b variation and morphological features of isolated Banded Pygmy Sunfish (Elassoma zonatum) populations in the lower Green River and Coastal Plain systems in Western Kentucky
… with genetic comparisons using mitochondrial cytochrome-b (Cyt-b) were conducted to test relationships between populations. Two sites, Cypress Creek in the Lower Green River system and Rose Creek in the Tradewater River drainage, were sampled from above the inner boundary and two additional …
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Identification and Characterization of the Cyd Gene Locus of Escherichia Coli (Respiration, Membranes, Cloning, Energetics, Cytochromes)
… of Escherichia coli contains a terminal oxidase, cytochrome d, which is part of a two subunit protein complex that is composed of two additional cytochromes, b(,595) and b(,558). The first mutants affecting the cytochrome d complex (Cyd) have been isolated using two newly developed genetic …
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A macro- and micro-evolutionary investigation of African Camponotus ants
Camponotus than the cytochrome oxidase II gene, based on almost all measures of phylogenetic utility. The primary hypothesis proposed to account for this observation is that these two mitochondrial genes are evolving under different evolutionary constraints. Specifically, the cytochrome oxidase II …
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Surface immobilization of engineered hemeproteins: Orientation, stability, structure, and function
… applications. Hemeproteins, including myoglobin, cytochrome b$\sb5$ and cytochrome b$\sb{562}$ that are used in this study, are excellent candidates for the fabrication of novel biomaterials due to their unique electronic and optical properties. This work shows that protein engineering technology …
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Macromolecular recognition in the cytochrome P450(cam) enzyme system
The cytochrome P-450$\sb{\rm cam}$ reaction cycle is a complex set of coordinated chemical transformations requiring precise temporal and spatial control of reactivities. Cytochrome P-450$\sb{\rm cam}$ catalyzes the regio- and stereo-specific hydroxylation of camphor to form 5-exo-hydroxycamphor. …
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CHEMICAL AND PHYSICAL CHARACTERIZATION OF COMPLEX III; THE OXIDATIVE REACTION MECHANISM
… compounds with isolated and with mitochondrial cytochrome b using MCD and EPR showed that the unusual coordination structure of the cytochrome b heme(s) can be represented by hindered bis-imidazole protoheme. The midpoint potentials for the b and c(,1) cytochrome were measured using MCD and EPR. …
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Molecular Studies of the Succinate:ubiquinone Oxidoreductase of Escherichia Coli
… centers. The small hydrophobic peptides contain cytochrome $b\sb{556},$ anchor the hydrophilic domain to the membrane, and allow electrons to be transferred to ubiquinone in the aerobic respiratory chain. The role of the b heme has eluded investigators for years. This work focuses on elucidating …
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Blood Meal Analysis for the Detection and Identification of Host DNA in the Gut Contents of Ornithodoros coriaceus
… mitochondrial genes, 18S rDNA, 12S rDNA, and cytochrome b, were used to detect host DNA in the gut contents of the soft tick vector. Polymerase chain reaction in combination with reverse line blot showed that the cytochrome b marker could accurately identify host DNA in ticks, fed …
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Parallel instrumentational approaches to the study of the bioenergetics of photosynthesis in chloroplasts and intact plants
… midpoint potentials of the chromophores of the cytochrome b6f complex (b6f) under a variety of redox and inhibitory conditions designed to help elucidate its function in electron transfer. These studies, were directed at settling controversies about the properties and function of the chloroplast …
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Phylogeographic analysis reveals strong geographical structuring in the klipspringer, Oreotragus oreotragus
… designations. Mitochondrial control region and cytochrome b sequence data were used to investigate these topics using blood, faecal and museum specimens. Cytochrome b and control region fragments were amplified in 83 and 60 samples respectively, out of a total sample set of 89 individuals. The …
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Phylogeographic structure of the Atlantic pupfish, Cyprinodon variegatus (Cyprinodontidae), along the eastern coast of North America: Evidence from mitochondrial nucleotide sequences
… (D-loop, non-coding origin of replication) and cytochrome-b gene for evidence of northern and southern subspecies within the Atlantic Coast of the eastern United States. Additionally, it will be may be possible to determine if secondary hybrid zones developed as a result of the last retreat of …
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Characterization of the composition and activity of the photosynthetic apparatus in chloroplasts
… and inactive photosystem II, photosystem I, cytochrome b/f, high-potential cytochrome b-559, the rate of uncoupled electron transport, and the ratio of chlorophyll a to b. In leaves the amounts of the photosynthetic components per leaf area, the fresh weight per leaf area, the rate of …
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Experimental and computational investigations of the stability and dynamics of cytochrome bb5s
… and experimental approaches were used to study cytochrome b$\sb5.$ The goal was to investigate protein dynamics and how they may relate to stability and biological function.Computational studies used molecular dynamics simulations to model the solution structure of cytochrome b$\sb5.$ …
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Evolution of the African Tigerfish (Genus Hydrocynus) : phylogeographic insights into drainage evolution
… framework and divergences are dated using the cytochrome b (cyt) region.
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