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Showing 1 to 13 of 13 for “"cytochrome P450 monooxygenase"”.
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Circadian regulation and phytohormone responses of cytochrome P450 monooxygenase genes in Arabidopsis
… and the time-of-day-specific transcripts of cytochrome P450 monooxygenases (P450s) involved in their syntheses and catabolisms suggest an interactions between hormone signaling network and circadian signaling network. The first phase of my research profiled a total of 98 P450 genes in …
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Isolation and characterization of cytochrome P450s from Papilio polyxenes and Papilio glaucus
Previous studies have isolated two cytochrome P450 monooxygenase cDNAs designated CYP6B1v1 and CYP6B1v2 from the oligophagous Papilio polyxenes. Baculovirus-mediated expression of these P450s demonstrated that both enzymes metabolize substantial amounts of linear furanocoumarins and low, if any, …
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The 21-dehydroxylation of corticosteroids: evidence for an enol intermediate and the hydroxylation of steroids by fungal cyto-chrome P450: evidence for a stepwise mechanism
… tum, and the hydroxylation of steroids by fungal cytochrome P450. Deuterium labelling techniques were used to study the enzymic dehydroxylation. Corticosteroids doubly labelled (2H) at the C-21 position were incubated with a culture of Eubacterium lentum. It was found that t he enzymic …
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Examining Molecular Interactions of Proteins by Isotopic Labeling, Sample Formulation and Solid-State NMR Spectroscopy
… methods of two large membrane proteins, E. coli cytochrome bo3 oxidase and A. thaliana cytochrome P450 monooxygenase 98A3. To obtain site-specific resolution in uniformly- 13C, 15N labeled samples of these proteins, hardware advances, new experimental techniques and increased dimensionality were …
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Comparative structural analysis of cytochrome P450 family CYP51 in the genus Cryptococcus
Cytochrome P450 monooxygenase CYP51 (sterol 14α-demethylase) is a well-known target of the azole drug fluconazole for treating cryptococcosis, a life-threatening fungal infection in immune-compromised patients in poor countries. Studies indicate that mutations in CYP51 confer fluconazole resistance …
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Effect of cell- and species-differences in the expression of drug metabolism enzymes on chemical-induced injury to the rat and human kidney
… caused in part by differences in the activity of cytochrome P450 monooxygenase (P450) between the two cell types. rPT cells expressed cytochrome P450 (CYP) 2B1/2, CYP2C11, CYP2E1, CYP4A2/3, and GSTα, but not CYP3A1/2, GSTµ, GSTπ, or GSTτ. rDT cells expressed CYP2B1/2, CYP2E1, and CYP4A2/3, GSTα, …
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Symbiont-Mediated Modification of Mosquitocide Toxicity in the Dengue Vector, Aedes aegypti
… increased susceptibility and a reduction in cytochrome P450 monooxygenase and general esterase activities when treated with naled and propoxur. A reduction of bacteria in mosquito larvae treated with broad-spectrum antibiotics, therefore, appears to affect the metabolic detoxification of …
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Biochemical and Functional Characterization of Induced Terpene Formation in Arabidopsis Roots
… foliage. We have identified a single cytochrome P450 monooxygenase of the CYP705 family that catalyzes a root-specific oxidative degradation of the C30-triterpene precursor arabidiol thereby causing the release of DMNT and a C19-degradation product named arabidonol. We found that DMNT …
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Structural Biology of Cytochrome P450 Monooxygenases in Arabidopsis Thaliana Phenylpropanoid Pathway
Plant genomes are rich in cytochrome P450 monooxygenase sequences with 246 full-length genes found in the Arabidopsis thaliana genome. Unlike other enzymes that catalyze substrate modifications, P450s catalyze the activation of oxygen, which then reacts with the nearest substrate atom. Among the …
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Reactor design for the hydroxylation of n-octane using a cyp153a6-based biocatalytic system expressed in Escherichia coli
… to 1-octanol using a previously developed cytochrome P450 monooxygenase, CYP153A6, enzymatic system. The biocatalyst was expressed in Escherichia coli BL21(DE3) by using a pET28b-PFR1500 plasmid encoding the complete operon from Mycobacterium sp. HXN-1500 which included the ferrodoxin …
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Insecticide detoxification in the navel orangeworm Amyelois transitella (Lepidoptera: Pyralidae)
… dietary studies the effects were assessed of the cytochrome P450 monooxygenase (P450) inhibitor piperonyl butoxide and the glutathione-S-transferase (GST) inhibitor diethyl maleate (DEM) on the toxicity of the insecticides azinphos-methyl, chlorpyrifos, chlorantraniliprole, β-cyfluthrin, and …
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A P-glycoprotein-like mechanism in the nicotine-resistant insect, Manduca sexta.
… parallel study, a monoclonal antibody against a P450 from a DDT-resistant housefly (Waters et al., 1989) was used to localize a cytochrome P450 monooxygenase enzyme (P450 or PSMO) to the M. sexta midgut. The co-localization of P450 and P-glycoprotein to the midgut suggests that larval M. sexta …
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Impacts of organic and conventional neurotoxic pesticides on a pest and a pollinator in almond agroecosystems (Prunus dulcis)
… enhancement of toxicity. The effects of the cytochrome P450 monooxygenase (P450) inhibitor piperonyl butoxide and the glutathione-S-transferase inhibitor diethyl maleate on the toxicity of the insecticides acetamiprid, clothianidin, and spinosad (a natural fermentation product made up of a …