Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 36 for “"cytidine deaminase"”.
-
The Role of Activation-Induced Cytidine Deaminase in Antibody Diversification and B Cell Malignancies
<p>Activation-induced cytidine deaminase (AID) is indispensable for somatic hypermutation (SHM) and class switch recombination (CSR) in B cells. SHM alters the affinity of an antibody for its corresponding antigen by introducing point mutations within the variable region of the immunoglobulin (Ig) …
-
Investigation of the role of the DNA-modifying enzyme, activation-induced cytidine deaminase (AID), in cancer
The DNA-editing enzyme, Activation-Induced cytidine Deaminase (AID) is essential for antibody diversification and plays an important role in immunity. AID is specifically expressed in activated B-cells and mutates targeted DNA sites, diversifying the antibody repertoire. Due to its mutagenic …
-
The Effect of Activation Induced Cytidine Deaminase Phosphorylation and Herpes Virus Uracil Dna Glycosylase On Antibody Diversification
<p>Activation-induced cytidine deaminase (AID) is a mutagenic enzyme that is expressed in mammalian B-cells and initiates the antibody diversification processes of somatic hypermuntation (SHM) and isotype class switch recombination (CSR). AID is targeted to the immunoglobulin gene locus where it …
-
An investigation of the impact of the B cell lymphoma factor Activation Induced Cytidine Deaminase (AICDA/AID) on epigenetic plasticity
… of the DNA modifying enzyme Activation-induced cytidine deaminase (AICDA) is a factor linked to cancer progression, particularly B-cell derived non-Hodgkin lymphomas, but has also been associated with other inflammation-associated cancers, including colorectal cancer (CRC). The widely recognised …
-
Genome-Wide Characterization of the Effects of Nucleic Acid Modifying Enzymes: Cytidine Deaminases and DNA Methylation
<p>Activation-induced cytidine deaminase (AID) is essential for two processes of immunoglobulin diversification in germinal center B cells: somatic hypermutation (SHM), in which mutations are introduced into immunoglobulin (Ig) genes, and class-switch recombination (CSR), in which genomic constant …
-
The Extent and Nature Of Chromosomal Rearrangements in B Lymphocytes
… This may be the result of Activation Induced Cytidine Deaminase (AID) expression, which introduces a heretofore uncharacterized array of rearrangements. Despite the importance of these events, current understanding of their genesis is limited. To examine the origins of chromosomal …
-
The Role of RNA Helicase RHAU in Immunoglobulin Class Switch Recombination
… (CSR). In these cells, activation induced cytidine deaminase (AID) binds Gquadruplex (G4) switch transcripts, which serve as guide RNAs to target AID to the immunoglobulin heavy chain switch (S) regions in the DNA for CSR. Sequence alignment revealed homology between the AID G4 binding …
-
A High-Throughput Approach to Uncover Novel Roles of APOBEC2, a Functional Orphan of the AID/APOBEC Family
<p>APOBEC2 is a member of the AID/APOBEC cytidine deaminase family of proteins. Unlike most of AID/APOBEC, however, APOBEC2's function remains elusive. Previous research has implicated APOBEC2 in diverse organisms and cellular processes such as muscle biology (in Mus musculus), regeneration (in …
-
Regulation of Immunoglobulin Gene Diversification By Noncoding RNA's
… processes are catalyzed by activation-induced cytidine deaminase (AID), a potent DNA mutator whose expression and function are highly regulated. I show that AID is regulated posttranscriptionally by a lymphocyte-specific microRNA, miR-155. I find that miR-155 is upregulated in murine B …
-
The Role of ZMYND8 in Immunoglobulin Class Switch Recombination
… is initiated by the enzyme activation induced cytidine deaminase (AID), which produces nucleotide mismatches in actively transcribed immunoglobulin heavy chain (Igh) switch donor and acceptor DNA. The 3' Regulatory Region (3'RR), a prototypical super-enhancer located at the 3' of the Igh locus, …
-
Identification and Characterization of APOBEC1 mRNA Editing Targets: A Transcriptomics Approach
… B mRNA Editing Catalytic polypeptide 1 (APOBEC1) cytidine deaminase is an mRNA editing enzyme that modifies a specific cytidine in the apolipoprotein B (apoB) transcripts of small intestine enterocytes. APOBEC1-mediated cytidine to uridine editing generates an inframe stop codon and results in …
-
Functional assessment of BRAF and CRAF through Base Editor 3 mutagenesis reveals residues and regions critical for ERK1/2 signalling
… tool comprised of a nickase Cas9 fused to a cytidine deaminase and a uracil DNA glycosylase inhibitor. This can be used to generate high-efficiency C to T mutations. This thesis describes the use of a high efficiency BE3 mutation screen in an arrayed format with the high-throughput functional …
-
Identification and Characterization of Drug Targets in the Pyrimidine and Purine Pathways of Trypanosoma brucei
… redundant enzymes: thymidine kinase (TK) and cytidine deaminase (CDA). Using a combination of genetic and analytical techniques, a novel pathway linking cytosine and thymine nucleotides was discovered. This pathway is composed of the salvage enzymes TK and CDA in addition to a newly discovered …
-
Structure-Function Studies on Aspartate Transcarbamoylase and Regulation of Pyrimidine Biosynthesis by a Positive Activator Protein, PyrR in Pseudomonas putida
… nucleoside kinases, uridine phosphorylase and cytidine deaminase. Without a functional nucleoside kinase, it was impossible to feed exogenous uridine to P. putida. To obviate this problem, uridine kinase was transferred to P. putida from E. coli and shown to function in this heterologous host. …
-
Restriction of Retrotransposition and Retroviral Infection by RNase L and APOBEC3G
… Virion-associated A3G exhibited elevated cytidine deaminase activity in the presence of RNase L. These results supported a regulatory mechanism for A3G during HIV-1 infections whereby RNase L plays an activating role. Finally in Chapter 5, I determined the in vivo hypermutation …
-
Biochemical and Structural Studies of RNA and DNA Editing Enzymes
… occurring by way of hydrolytic deamination of cytidine (C) and adenosine (A), is a modification event that profoundly effects on an organisms vitality and quality of life. This study focuses on two types of enzymes---ADAT (adenosine deaminase acting on tRNA) and APOBEC3G (apolipoprotein B …
-
Single-Stranded Dna Scanning And Deamination With Single-Molecule Resolution
… <p>APOBEC3G (Apo3G) and Activation-Induced cytidine Deaminase (AID) are the most notable members of APOBEC enzymes, a family of cytidine (C) deaminases with crucial functions in the immune system. In T cells, Apo3G deaminates C in viral cDNA to halt the replication of HIV 1 strains lacking …
-
Structure Function Analysis of Adenosine Deaminase Acting on tRNA (ADAT2) from Trypanosoma Brucei
… is carried out by the homodimeric adenosine deaminase acting on tRNA (ADAT) and modifies only tRNAarg, whereas in eukarya the enzyme is the ADAT2/3 heterodimer, which performs the same reaction on all tRNA molecules with adenosine at position 34. Despite a similar evolutionary origin, the …
Page 1 of 2