Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"cysteine desulfurase"”.
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Structural insight into the assembly of iron-sulfur clusters and their function in radical generation
… of radical chemistry. The structure of a cysteine desulfurase involved in cluster bioassembly in the cyanobacterium Synechocystis PCC sp. 6803 was solved by X-ray crystallography and analyzed in terms of its mechanistic implications. We found that the active site cysteine responsible for …
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Developing Budding Yeast as a Cell Factory for Production of the Antioxidant Ergothioneine
… To complete the EGT biosynthetic process, a cysteine desulfurase is required, and two candidates were identified in A. fumigatus, namely AFUA_2G13295 and AFUA_3G14240, termed egt2a and egt2b in this study. Co-expression of egtA and egt2a in S. cerevisiae using high expression 2μ vectors was …
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Cysteine Desulfurases Mediating Sulfur Trafficking for the Biosynthesis of Fe-S Clusters
… to date include the participation of at least a cysteine desulfurase and an Fe-S cluster scaffold. The first step in sulfur mobilization for the assembly of Fe-S clusters as well as other thiocofactors within the cell involves a PLP-dependent enzymatic mechanism, catalyzed by cysteine …
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Characterization Of Initial Iron Binding Location And The Structure/iron Binding Site On S.cerevisiae Isu And On D.melanogaster Frataxin
… provider and an allosteric modulator of both the cysteine desulfurase and scaffold protein ISU. My research has been focused on studying the interaction between frataxin and ISU, particularly the mechanism of iron binding/ iron acceptance by ISU from frataxin. Using XAS, the Stemmler laboratory …
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FUNCTIONAL STUDIES OF THE FE-S CLUSTER BIOGENESIS BY SUF SYSTEM
… step of sulfur mobilization is catalyzed by the cysteine desulfurase SufS, which transfers the sulfur from the free amino acid cysteine to specific sulfur acceptor proteins. Despite similarities in their general reaction schemes, the proteins and mechanisms involving the second reaction step are …
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The Biosynthesis and Function of Nitrogenase Metalloclusters
… of both nitrogenase components. NifS is a cysteine desulfurase that provides the sulfur for cluster formation and NifU serves as a molecular scaffold during [Fe-S] cluster assembly. Genetic and biochemical experiments involving amino acid substitutions within the N-terminal and C-terminal …
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Biochemical and genetic characterization of mercaptopyruvate sulfurtransferase and paralogous putative sulfurtransferases of Escherichia coli
… could participate or work in concert with a cysteine desulfurase, IscS, in the biosynthesis of cofactors was examined. Cloning of the sseA gene and biochemical characterization of the corresponding protein were used to show that SseA is a mercaptopyruvate sulfurtransferase of E. coli. A …
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Investigations of conserved structure-function relationships and enzymatic evolution
… stress and iron starvation. SufS is the cysteine desulfurase enzyme responsible for the acquisition of sulfur and subsequent transfer to transpersulfurase SufE. Recent studies have provided several crystal structures of SufS including two variants displaying stalled intermediates of the …
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Isolation of in vivo intermediates in iron sulfur cluster biogenesis
… two central <i>isc</i> players include IscS, a cysteine desulfurase, and IscU the proposed site of iron-sulfur cluster assembly. Using <i>A. vinelandii</i> as a model organism, we have sought to better understand the mechanism of <i>in vivo isc</i> cluster assembly. In order test the scaffold …
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Biosynthesis of Iron-Sulfur Clusters
… The NifS protein has been shown to have cysteine desulfurase activity and can be used to supply sulfur for the <I>in</I> <I>vitro</I> catalytic formation of [Fe-S] clusters. The activity of the NifU protein has not yet been established, but NifU could have functions complementary to NifS …
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Molecular Details Of The Mitochondrial Iron Sulfur Cluster Assembly Pathway
… site on ISU scaffold proteins other than the cysteine rich active site.</p>