Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 82 for “"cycloheximide"”.
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Resistance to protein synthesis inhibitors in Coprinus cinereus
Cycloheximide, a potent eukaryotic protein synthesis inhibitor (Sisler and Siegel, 1967) was used in a biochemical and genetical investigation of the beisidiomycete fungus, Coprinus cinereus. An optimised polyuridylic acid dependant cell-free polyphenylalanine synthesising system was developed for …
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Rapid turnover of McL-1 couples translation to cell survival and apoptosis
… of translation induces apoptosis using the drug cycloheximide as a tool to isolate and study the direct effects of translation inhibition. Cycloheximide-induced cell death was detected within 2 - 4 hours of treatment in various cell types. Pre-treatment with several proteasome inhibitors blocked …
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Rapid Alterations in Rat Brain Tryptophan Hydroxylase Activity; Effects of Adrenal Steroids, Drugs and Stressors
… rats. This increase was blocked by cycloheximide, a protein synthesis inhibitor. The midbrain enzyme activity was increased in normal rats by short term stressors--cold (5° C), ether, and electric foot-shock--which resulted in increase plasma corticosterone levels. Adrenalectomized …
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Heat shock protein 27 inhibition of apoptosis in human breast cancer cells
… Butyrate, Staurosporine, Vitamin E Succinate and Cycloheximide. The cells that constitutively expressed Hsp 27 had a higher growth in the presence of Cycloheximide and Vitamin E Succinate and also exhibited lesser apoptosis in the presence of Sodium Butyrate and Staurosporine. This study …
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Nuclear Transfer With Porcine Embryonic Stem Cells
… and electroactivation + culture with cycloheximide improved activation rates, and (3) electroactivation + culture with cycloheximide was the most efficient treatment for production of pronuclear stage cytoplasm. Results from NT experiments indicated that neither enucleated, unactivated …
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Investigating temperature signalling pathways in Arabidopsis thaliana using small molecules
… In addition, the translation inhibitor cycloheximide was identified as a repressor of heat-inducible genes and an activator of early cold-inducible genes. Diverse translation inhibitors trigger a cytosolic influx of calcium ions and several inhibitors of translation elongation were found …
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The Control of Spore Germination in Anemia Phyllitidis (L.) Swartz and Other Ferns
… not identical to any of the known gibberellins. Cycloheximide inhibits the gibberellin-induced germination and also inhibits incorporation of C<sup>14</sup>-amino acids into TCA-insoluble material. Actinomycin D has no effect on germination, but it is not shown whether the inhibitor enters the …
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Regulation of tissue levels of metallothionein with emphasis on metallothionein degradation
… inversely proportional to cellular Zn status. Cycloheximide, chloroquine and tosyl-lysine chloromethyl ketone (TLCK) inhibited ³⁵S-MT degradation by 33, 65 and 50%, respectively, without affecting cellular Zn status. Degradation of ³H-protein was inhibited by 41, 41 and 16% in the presence of …
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Experimental evidnece for hysteresis in the cell cycles of Xenopus Laevis egg extracts
… of nondegradable cyclin B1 were added to cycloheximide-treated CSF-released extracts, and samples taken at various time-points were analyzed for MPF activity. At 40 nM cyclin B1, just above the activation threshold, the lag time for MPF activation was 45 - 60 minutes; at 50 nM cyclin B1, …
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Identifying Novel Interaction Between the E3 Ubiquitin Ligase Ring Finger Protein 20 (RNF20) and the Deubiquitinating Enzyme Ubiquitin Specific Protease 7 (USP7)
… with USP7 or the catalytic mutant USP7, cycloheximide chase and a deubiquitination assay, we show that RNF20 stability is dependent upon USP7 removing ubiquitin chains that would mark RNF20 for proteasomal degradation. This interaction was seen within multiple cell lines, with the …
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Utilization of Poly(A)-Binding Protein in Monitoring the Formation of mRNP Granules in Toxoplasma gondii
… and in intracellular parasites whereas cycloheximide can diffuse these granules in less than one hour.
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Regulation of Protein Turnover during Hyper-osmotic Stress in Skeletal Muscle
… media (Gibco). L-[14C]-U-phenylalanine (n=8) and cycloheximide (n=8) were used to quantify protein synthesis and degradation, respectively. Western blotting analyses was performed to determine the activation of protein synthesis and degradation pathways. During hyperosmotic stress, protein …
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Protein Turnover in Rat Skeletal Muscle During In Vitro Hypo-Osmotic Stress
… 75 min incubations with L-U[14C]phenylalanine or cycloheximide to determine protein synthesis and degradation. Immunoblotting was performed to assess signalling pathways involved. Phenylalanine uptake and incorporation were increased by 199% and 169% respectively in HYPO from ISO (p < 0.05). This …
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Interrelationships among estrogen, insulin-like growth factor-I and cyclic adenosine monophosphate in the regulation of uterine progesterone and estrogen receptors
… by pre-treatment of cells with actinomycin D or cycloheximide. These results suggest that regulation of PR occurs at the level of the PR gene, and that induction of PR stimulated by these agents requires activation of estrogen receptor (ER) and the action of protein kinases. Treatment with cGMP …
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Re-evaluation of the hypothesis that LTP has two temporal phases and that the late phase is protein synthesis-dependent
… slice by long-lasting pretreatment with the PSI cycloheximide deteriorated the stabilization of LTP. Additionally, acceleration of protein degradation using hydrogen peroxide after the induction of LTP resulted in decay of LTP. Addition of cycloheximide induced additive decay of LTP …
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Translation and transcription are required for endogenous bursting after long term removal of neuromodulators
… is exposed to the protein synthesis inhibitor Cycloheximide (CHX) and the transcription inhibitor Actinomycin D (ACD). The results suggest that early transcription and late protein synthesis play an important role in the recovery process of the rhythmic activity in the pyloric network of the …
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Degradational and Transcriptional Investigations of the RAF Kinase Inhibitory Protein
… mechanisms using ubiquitination and cycloheximide blocking experiments or transcriptional mechanisms other than by Snail repression using luciferase reporter assays. My results indicate that RKIP is a relatively stable protein, which has a half-life around 1 to 2 days and is not …
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Degradational and Transcriptional Investigations of the RAF Kinase Inhibitory Protein
… mechanisms using ubiquitination and cycloheximide blocking experiments or transcriptional mechanisms other than by Snail repression using luciferase reporter assays. My results indicate that RKIP is a relatively stable protein, which has a half-life around 1 to 2 days and is not …
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Methods to Detect Apoptosis in Equine Peripheral Blood Neutrophils from Normal Healthy Adult Horses
… of apoptosis; actinomycin D, staurosporin, cycloheximide and sodium hypochlorite, at four different concentrations (table 2). A fifth population was to induce necrosis using a freeze-thaw cycle and bleach. A control sample was examined (no inducer) to determine spontaneous rate of apoptosis. …
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The role of nerve growth factor and p75 neurotrophin receptor in recovery from liver fibrosis
… myofibroblasts from serum-deprivation and cycloheximide-induced apoptosis. To define the dominant effect of p75 neurotrophin receptor-mediated events in experimental liver fibrosis I have used a mouse lacking the p75 neurotrophin receptor ligand-binding domain but expressing the …
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