Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"cyclin B1"”.
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Cyclin B1 Mediates The Effect of Uchl1 In Promoting Cell Cycle Progression In Uterine Papillary Serous Carcinoma
… was due to its ability to stabilize cyclin B1 protein, an essential protein in mitotic progression. Specifically, we demonstrated that UCHL1 and cyclin B1 interact with each other in both the cytoplasm and nuclear space prior to mitosis. UCHL1 silencing increased the deubiquitination …
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Experimental evidnece for hysteresis in the cell cycles of Xenopus Laevis egg extracts
… <u>Prediction 1: The threshold concentration of cyclin B required to activate MPF is measurably higher than the threshold concentration required to inactivate MPF.</u> The difference in thresholds implies that the MPF control system is hysteretic and bistable. To measure these thresholds, …
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Differenzielle Regulation und prognostische Bedeutung von zellzyklusassoziierten Regulatoren der G1- und G2-Phase in Abhängigkeit von der anatomischen Lokalisation in Gastrointestinalen Stromatumoren (GIST)
… von den G1-Phase-Proteinen E2F1 und Cyclin D1, sowie von den G2-Phase-Proteinen Cyclin A2 und Cyclin B1 und dem Transkriptionsfaktor Myc wurden in Abhängigkeit von den klinisch-pathologischen Parametern Lokalisation, Morphologie, Tumorgröße, Mitosenanzahl und Mutationsstatus …
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Unterschiedliche Aktivierung von Signalwegen zur Zellproliferation in mesenchymalen Tumoren des Gastrointestinaltrakts
… Tumoren mehr in GIST exprimiert. Während die Cyclin-D1-Expression der GIST höher war, wurden die höchsten E2F1- und Cyclin-B1-Expressionen bei den Leiomyosarkomen gefunden.SCHLUSSFOLGERUNGEN:Im Vergleich zu Leiomyomen weisen Leiomyosarkome PDGFRA-Expression auf. Für GIST kann eine sequentielle …
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Exploring the effects and efficacy of novel cell cycle regulating compounds in aggressive Breast, Ovarian and Cervical Cancer
… investigate the expression levels of Cdc20 and Cyclin B1 to confirm the compounds were acting on the intended target. The novel compounds were found to reduce cell viability in the cancer cell lines tested but were also very cytotoxic to the non-carcinomic RPE-1 cells. The cytotoxicity …
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Prognostic and treatment predictive factors for radio- and chemotherapy resistance in breast cancer patients- a step towards personlized medicine
… prognostic importance of a proliferation marker, cyclin B1,in a case-control study. There were 190 lymph node negative breast cancer patients with no chemotherapy who died from breast cancer and 190 corresponding controls who were alive at the corresponding case’s time of death. Cyclin B1 was an …
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Eukaryotic chromosome segregation: New aspects of separase regulation by securin, Cdk1, PP2A and auto-cleavage
… inactive by association with either securin or cyclin-dependent kinase 1 (Cdk1) in conjunction with cyclin B1. Only when all chromosomes interact properly with the mitotic spindle apparatus does the anaphase promoting complex or cyclosome (APC/C), a multisubunit E3 ligase, mediate the …
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THERAPEUTIC POTENTIAL OF TARGETING THE FLNA-REGULATED WEE1 KINASE PATHWAY IN ADRENOCORTICAL CARCINOMAS.
… and in primary ACC cells. Higher p-CDK1 and cyclin B1 were shown in FLNA-silenced MUC-1, while decreased Wee1, p-CDK1 and cyclin B1 resulted after FLNA overexpression. Notably, Wee1 depletion was reverted by lactacystin treatment, and FLNA transfection increased Wee1 phosphorylation at Ser123 …
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Characterisation of the molecular complexes that regulate the G2/M checkpoint of the eukaryotic cell cycle
… progression is principally mediated by the CDK1/cyclin B1 complex; phosphorylation of CDK1 by wee1 kinase prevents progression to mitosis, and subsequent dephosphorylation by the CDC25 phosphatases, initially the B isoform, leads to mitotic onset. The aim of this research was the biophysical …
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Developing Fluorescent Tools to Dissect the Role of Tuberin at Mitotic Onset
… binding to and controlling the localization of Cyclin B1 (Cyc B1). We have determined that phosphorylation of five key residues in the cytoplasmic retention sequence (CRS) of Cyc B1 decreases this binding interaction and permits the accumulation of Cyc B1 in the nucleus and mitotic onset to …
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The peptidyl-prolyl isomerase Pin1 is required for maintenance of the spindle assembly checkpoint
… degradation of its two inhibitors, securin and cyclin B1, which is mediated by the anaphase promoting complex or cyclosome (APC/C), a multisubunit ubiquitin ligase, in conjunction with its essential co-activator Cdc20. The spindle assembly checkpoint (SAC) is a surveillance mechanism that …
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Harnessing the Anaphase-Promoting Complex for Targeted Protein Degradation
… of numerous proteins including Securin, Cyclin B1 and Aurora kinase A. Most natural APC/C substrates contain multiple copies of short linear motifs (SLiMs), termed degrons, that serve as APC/C-recognition sequences. My research approach involved systematically dissecting various …
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The Role of CDCA7 in Akt-mediated Myc-Dependent Apoptosis and Proliferation
… the proapoptotic BAX promoter, elevates BAX and Cyclin B1 mRNA levels while reducing p15INK4B mRNA levels. This data points to a novel mechanism which implicates Akt phosphorylation of CDCA7 as participating in the dual signal model of Myc of function and thus affecting Myc-dependent growth and …
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Decoding Lysine-11 Signals in Ubiquitination
… degradation of the cell cycle substrate, cyclin B1. To further explore the functions of K11-chains I focused on the hypoxia inducible transcription factor (HIF) pathway, as K11-ubiquitination had been implicated in proteasome-independent degradation of the transcription factor. I …
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New Roles of the Transcription Factor NKX6.1 in Beta Cell Biology
… in part via direct interaction with the cyclin B1 gene and stimulation of its expression. Thus, whereas the majority of primary beta cells are quiescent, overexpression of Nkx6.1 is sufficient to initiate DNA synthesis and cell division. Importantly, and different from the common …
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Molecular mechanisms of Kruppel-like Factor 4
… acetylation. On the negatively regulated gene, Cyclin B1, I demonstrate that KLF4 recruits HDAC3, decreasing histone acetylation. In addition, I show that KLF4 is acetylated by p300/CBP, and that acetylation of KLF is important in the activation of target genes as well as its ability to inhibit …
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Exosomal Survivin-T34A: A Novel, Potential Cancer Therapeutic
… abolishes a phosphorylation site for p34cdc2-cyclin B1 resulting in the initiation of the mitochondrial apoptotic pathway in cancer cells with little to no direct effects on normal cells. The possibility that targeting Survivin in this manner may provide a novel approach for selective cancer …
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Sulforaphane Inhibits Mammary Cancer Cell Mitotic Progression and Tubulin Polymerization
… (P < 0.05) G 2/M cell cycle arrest and elevated cyclin B1 protein within 24 h. Moreover, 15 muM SUL was found to significantly (P < 0.05) induce phosphorylation of histone H1, block cells in early mitosis, and disrupt polymerization of mitotic microtubules in vivo. Taken together, these findings …
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Mechanism of APC/C activation and substrate specificity in mitosis
… hyper-phosphorylation of APC/C subunits by cyclin-dependent kinase (Cdk) and polo kinase. The aim of the first part of this thesis was to understand how mitotic phosphorylation regulates APC/C activity. Using cryo-electron microscopy and biochemical analysis, we found that an auto-inhibitory …
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HDAC inhibition results in suppression of FOXM1 and genes associated with poor prognosis prostate cancer through p53 dependent and independent mechanisms
… suppression of critical cell cycle genes such as Cyclin B1 and FOXM1. Therefore, Entinostat induces a p53-independent suppressive role of p21 that essentially mimics the activation of p53. <br/><br/>Genome-wide transcriptomic analyses confirmed that Entinostat mimics p53 mediated suppression of …
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