Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"cyclin B"”.
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Evaluating the role of the fission yeast cyclin B Cdc13 in cell size homeostasis
… with cell size. In fission yeast, Cdc13 (cyclin B), an important activator of the core cell cycle kinase Cdc2 (CDK1), increases in concentration as cells grow. It has been proposed that the concentration of such cell cycle regulators serves as a proxy for cell size and makes cell cycle …
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Quantitative Analysis of a Cell Cycle Checkpoint in Xenopus laevis Cell-Free Egg Extracts
… or damaged DNA by inhibiting the activity of cyclin-dependent kinases (Cdks). In the Xenopus laevis embryo, checkpoints are not operational until the midblastula transition (MBT). Studies in cell-free egg extracts indicate that a threshold concentration of nuclei, which approximates the MBT …
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Regulation of Anaphase Promoting Complex/Cyclosome to Control M Phase Exit
… At the onset of anaphase, the APC/C targets cyclin B and securin for degradation, initiating chromosome separation and mitotic exit. Regulation of APC/C activity is critical for a functional cell cycle, and this is largely mediated by cytostatic factor (CSF) activity and the Spindle Assembly …
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Experimental evidnece for hysteresis in the cell cycles of Xenopus Laevis egg extracts
… <u>Prediction 1: The threshold concentration of cyclin B required to activate MPF is measurably higher than the threshold concentration required to inactivate MPF.</u> The difference in thresholds implies that the MPF control system is hysteretic and bistable. To measure these thresholds, …
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Mitotic slippage by ATP depletion and its mechanism
… of rounded mitotic cells, degradation of cyclin B, increase of cell attachment, and reassembly of nuclear membrane. The results of EdU incorporation assay and time-lapse imaging revealed that survived cells from mitotic slippage preceded next interphase, but finally died even in normal …
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Mitotic regulators and their effects on Drosophila : chromosome structure during development
… complex/cyclosome. Increasing levels of cyclin B, a known mitotic target of the APC/C, does not alter the timing of the transition, indicating that CYCLIN B is not the only APC/C target at the polyteny-polyploidy transition. In mitosis, activity of APC/C and POLO lead to the loss of …
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Cell Cycle Regulatory Roles of Estrogen Receptor Alpha (Erα) In Breast Cancer Cells
… ligand. Additionally, complex formation between cyclin B and ER α was shown by immunoprecipitation and led to the discovery that anaphase promoting complex (APC) is the E3 ligase for both cyclin B and ERα at the termination of M phase.<br /><br /> Our findings suggest that unliganded ERα has an …
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The effect of doxorubicin on the U2OS cell cycle
… arrest occurs. This is shown by lower levels of Cyclin B in the G2 phase during flow cytometry analysis and staining with PI. Apoptosis levels are monitored using cleaved Caspase 3 and cleaved PARP. The percentage of 10 [mu]M cells undergoing apoptosis increased steadily over 48 hours, while the …
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Identification and functional characterisation of novel APC/C interacting proteins
… target essential cell cycle regulators such as cyclin A, cyclin B, securin and S-phase kinase-associated protein 2 (SKP2) for proteasomal-dependent degradation. APC/C substrates and coactivators are aberrantly expressed in many cancers. It is thought that the APC/C can also regulate cellular …
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Analysis of the role of rab11-FIP3 phosphorylation during cytokinesis
… a key role in the delivery and targeting of recycling endosomes to the furrow; this is essential for completion of cytokinesis. FIP3 undergoes spatial and temporal dynamics during mitosis. During metaphase and early anaphase, FIP3 is largely cytosolic, with some localised to endosomal membrane …
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Role of retinoblastoma protein in the development of liver cancer
… and apyrimdinic sites. The regulation of three cyclins, cyclin A, cyclin B and cyclin E is found to lack governance. Analysis of the G2/M checkpoint proteins such as Gadd45a, MAD2 and CDC25a all show a dysregulation in their activities. In conclusion Rb loss leads to aberrant regulation of p53 …
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caffeine에 의한 HGF-induced G2 delay 촉진효과 확인 및 기전분석
… with the delay in cdk1 activation and in cyclin B nuclear localization. Western blot analysis also showed delayed degradation of cyclin A and B. ATM/ATR knock-down cells did not show the augmentation of HGF-induced G2 delay, strongly suggesting that inhibitory activity of caffeine on …
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Cell cycle-dependent regulation of the human RNA cap methyltransferase (RNMT)
… is phosphorylated at Threonine-77 (T77) by CDK1/Cyclin B in a cell cycle-dependent manner during G2/M-phase.<br/>RNMT T77 phosphorylation activates cap methyltransferase activity in vitro. Furthermore, it negatively regulates the interaction of RNMT with KPNA2 (Importin-a), which was found to …
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Creg1 And Its Enhancement Of P16ink4a-Induced Senescence
… of CREG1 and p16INK4a inhibits the expression of cyclin A and cyclin B by inhibiting promoter activity thereby decreasing mRNA and protein levels; these proteins are required for S-phase entry and G2/M transition. In conclusion, we are the first to find that CREG1 enhances p16INK4a-induced …
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Molecular Mechanisms of Mitotic Checkpoint Complex Assembly onto Kinetochores
… degradation of two key cell cycle regulators, cyclin B and securin. The MCC is composed of BubR1, Cdc20 and Mad2, and while their assembly is an intrinsically very slow process, in cells it is catalytically activated. Recent work points towards hierarchical recruitments of SAC proteins onto the …
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Anti-cancer Functions and Mechanisms of a pRb2/p130 Peptide Fragment
… able to inhibit the kinase activities of Cdk2/Cyclin E/A complexes. In vitro kinase assays showed the inhibition occurred in a dose-dependent manner. The half maximal inhibition concentration of the Spa310 in the kinase assay was 1.67mM. In addition, it has been shown that Spa310 peptide is …
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Creation and enhancement of a quantized hybrid computational model of cell cycle progression including DNA damage and repair mechanisms
… of cell cycle progression controlled by the cyclin proteins, and ultimately the genes that regulate their expression. This interaction network is modelled using a Boolean network with the concentration of the cyclin proteins calculated by ordinary differential equations, thus creating a …
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The proteomic analysis of resistance to anticancer therapy in human breast cancer
… western blotting. These targets included p-ERK, cyclin D2 and cyclin B 1. The technique was subsequently extended using the Labvision Antibody Microarray Kit (Neomarkers) to analyse the MCF7 parental cell line and the derivative (MCF740Gy) displaying significant resistance to radiotherapy. The …
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TRAF Regulation of Caspase-2-Dependent Apoptosis in Response to DNA Damage
… targeting critical pro-mitotic proteins, like cyclin B and securin, to promote mitotic exit. Although the function of the MCC is well understood, its regulation is not, especially in regard to protein phosphatases To investigate this, we activated the spindle checkpoint with microtubule …
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