Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"cyclin A"”.
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Regulation of Ciz1 by Cyclin A-Cyclin Dependent Kinase 2 (CDK2) mediated phosphorylation
… process, regulated by the activities of cyclin dependent kinases. Phosphorylation of target proteins regulates key transitions in the cell cycle contributing to accurate DNA replication and cell division. Here we evaluate the regulation of the protein Ciz1 by cyclin A-CDK2. Ciz1 promotes …
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Mechanisms of Cytokine-Induced IGF -I Insensitivity in Cancer Cells
… to induce accumulation of both E2F-1 and cyclin A, to hyperphosphorylate retinoblastoma (RB), and to induce enzymatic activity and association of Cdk2 with cyclin A. The cytostatic property of these cytokines was also shown by their ability to block IGF-I-stimulated luciferase activity of …
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Probing the Role of Nascent Helicity in Protein Function: p27kip1 as a Regulator of the Cell Cycle
… binds to and regulates the activity of cyclin-dependent kinases (Cdks) which are the master timekeepers of the cell division cycle. Members of the p27 family of proteins, also including p21 and p57, are called cyclin-dependent kinase inhibitors (CKIs). The amino terminal domain of p27 …
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Design of Non-ATP Competitive and Cell Cycle Specific CDK Inhibitors Targeting Cyclin Binding Groove
<p>CDK2/cyclin A and CDK4/cyclin D1 are validated targets for cancer drug discovery with ATP competitive compounds being the most common inhibitors. However, issues arising from the selectivity of current inhibitors have prevented clinical validation of these important targets. To generate cell …
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Role of MDM2 In Cell Growth Regulation
… overexpression does not prevent expression of cyclin D, cyclin D-CDK mediated phosphorylation of Rb or cyclin E in normal, immortal or tumor-derived cells. However, MDM2 down-regulates cyclin A expression specifically in normal cells, which is associated with G1 arrest. The domain of MDM2 …
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Analysis of markers of cell division cycle, apoptosis and autophagy flux in glioblastoma
… Mcm2, expressed throughout the cell cycle; Cyclin A, an S-phase cyclin; Geminin, a protein that prevents re-initiation; and Phosphohistone H3 (PHH3), a marker of mitosis. Apoptotic markers included two anti-apoptotic proteins, Bcl-2 and Bcl-xl; a pro-apoptotic protein, Bak; and a final …
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The Effect of Radiation and Repeated Sub-culturing on TGF-β1 Signaling in FRTL-5 Cells
… regulating the expression of its response genes; cyclin A and plasminogen activator inhibitor-1 (PAI-1) under our experimental conditions. To determine this we used luciferase reporter constructs containing promoters for cyclin A and PAI introduced to the cultures by transfection. PAI-1 production …
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Regulation of the anti-senescence factor, TBX2, by the UV stress signaling pathway and the mitotic cyclin dependent kinases
… can suppress senescence through repressing the cyclin dependent kinase inhibitors, p21wAF11 c1 P118011 and p19ARF. Both Tbx2 and Tbx3 have also been linked to several cancers primarily because their expression levels have been found to be deregulated in these cancers. However, despite the …
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Regulation of the anti-senescence factor, TBX2, by the UV stress signalling pathway and the mitotic cyclin dependent kinases
… can suppress senescence through repressing the cyclin dependent kinase inhibitors, p21wAF11 c1P118011 and p19ARF. Both Tbx2 and Tbx3 have also been linked to several cancers primarily because their expression levels have been found to be deregulated in these cancers. However, despite the pivotal …
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Establishing DNA combing to investigate DNA replication stress in vitro
… is largely controlled by the activity of cyclin dependent kinases (CDKs) and their cyclin binding partners. Here using an in vitro cell-free DNA replication system we analyse the interplay between Ciz1 and cyclin A/CDK2 in regulation of the initiation phase of DNA replication. This …
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Identification and functional characterisation of novel APC/C interacting proteins
… target essential cell cycle regulators such as cyclin A, cyclin B, securin and S-phase kinase-associated protein 2 (SKP2) for proteasomal-dependent degradation. APC/C substrates and coactivators are aberrantly expressed in many cancers. It is thought that the APC/C can also regulate cellular …
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Analysis of cyclin-CDK mediated regulation of the G1/S transition in post quiescent murine fibroblasts
… be identified. Here we investigate the roles of cyclin E and cyclin A in the G1 to S-phase transition and reconstitute initiation of DNA replication in cyclin depleted nuclei using an in vitro DNA replication system. Using a murine 3T3 fibroblast model, chemical and genetic inhibition of CDK …
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Role of retinoblastoma protein in the development of liver cancer
… and apyrimdinic sites. The regulation of three cyclins, cyclin A, cyclin B and cyclin E is found to lack governance. Analysis of the G2/M checkpoint proteins such as Gadd45a, MAD2 and CDC25a all show a dysregulation in their activities. In conclusion Rb loss leads to aberrant regulation of p53 …
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caffeine에 의한 HGF-induced G2 delay 촉진효과 확인 및 기전분석
… with the delay in cdk1 activation and in cyclin B nuclear localization. Western blot analysis also showed delayed degradation of cyclin A and B. ATM/ATR knock-down cells did not show the augmentation of HGF-induced G2 delay, strongly suggesting that inhibitory activity of caffeine on …
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Regulation des DNA-Polymerase-[alpha]-Gens durch den Transkriptionsfaktor YB-1
Y-box protein-1 involvement in cyclin A and B1 gene regulation has recently been demonstrated (Jurchott, K., Bergmann S., Stein U., Walther W., Janz M., Manni I., Piaggio G., Fietze E., Dietel M., and Royer H.D. (2003) J. Biol. Chem. 278, 27988-96). A more generalised role of this protein for …
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Expression of Leukemia/Lymphoma Related Factor (LRF) in Human Breast Carcinoma
… LRF also upregulated the expression of the cyclin A, down- regulated p21CIP/WAF expression and induced cell cycle arrest in the G2 phase. Further studies are warranted to determine the malignant or proliferative role of LRF in human breast carcinoma.
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Prognostic factors in soft tissue sarcoma. Tissue microarray for immunostaining, the importance of whole-tumor sections and time-dependence.
… IHC expression of multiple markers (Ki-67, p53, cyclin A, bcl-2, ß-catenin, CD44, and Pgp) in 218 malignant fi brous histiocytomas (MFH) and in 140 mixed STS. In the MFH series, tumor size and Ki-67, as the only IHC marker, provided independent prognostic information. In the mixed STS series …
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The role of anchorage in cell cycle control
… progression in G1, because of insufficient cyclin D1 induction to overcome the Rb/E2F checkpoint, accumulation of cyclin-dependent kinase inhibitors, and lack of cyclin A production. Because of the complexity of anchorage-dependent mechanisms involved, several changes are required for cells …
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Creg1 And Its Enhancement Of P16ink4a-Induced Senescence
… of CREG1 and p16INK4a inhibits the expression of cyclin A and cyclin B by inhibiting promoter activity thereby decreasing mRNA and protein levels; these proteins are required for S-phase entry and G2/M transition. In conclusion, we are the first to find that CREG1 enhances p16INK4a-induced …
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