Global ETD Search
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Showing 1 to 17 of 17 for “"cyclin/CDK"”.
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Funktion und Regulation von D- und E-Typ-Cyclin-Cdk-Komplexen während der Entwicklung von Drosophila
… durchlaufen wird. Die Aktivität von Cyclin E-Cdk(Cyclin-dependent kinase)2-Proteinkomplexen ist essentiell für den Eintritt in die S-Phase. Umgekehrt erfordert der Austritt aus der Zellproliferation zum korrekten Zeitpunkt in der Entwicklung die Inaktivierung von Cyclin …
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Analysis of cyclin-CDK mediated regulation of the G1/S transition in post quiescent murine fibroblasts
… be identified. Here we investigate the roles of cyclin E and cyclin A in the G1 to S-phase transition and reconstitute initiation of DNA replication in cyclin depleted nuclei using an in vitro DNA replication system. Using a murine 3T3 fibroblast model, chemical and genetic inhibition of CDK …
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B-cyclin/CDK Regulation of Mitotic Spindle Assembly through Phosphorylation of Kinesin-5 Motors in the Budding Yeast, <italic>Saccharomyces cerevisiae</italic>
… it has been known for many years that B-cyclin/CDK complexes regulate the assembly of the mitotic spindle and entry into mitosis, the full complement of relevant CDK targets has not been identified. It has previously been shown in a variety of model systems that B-type cyclin/CDK …
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Studies of the Kinetics of Cell Cycle Processes in S. Cerevisiae: The Molecular Basis of Start Irreversibility and Cyclin-Cdk Ordering of Mitotic Events
… central negative feedback oscillator comprising cyclin-CDK and its antagonist, APCCdc20. This oscillator is stabilized and tuned by positive feedback loops, and its frequency is modulated by checkpoint controls. Either by directly triggering events, or by entraining independent oscillators …
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Mitotic Exit Control in Budding Yeast: Regulators and Dynamics
… Cdc14 release and ME is controlled by mitotic cyclin-Cdk oscillation, the FEAR network including a non-proteolytic function of separase (Esp1), and the Mitotic Exit Network (MEN) indirectly activated by spindle elongation through cohesin cleavage by the proteolytic function of Esp1. The MEN …
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Peak Mitotic Cyclin Permits Mitotic Exit
… mitosis, and cytokinesis are all regulated by Cyclin-dependent kinase (Cdk). Cyclin/Cdk complexes promote replication origin firing and mitotic entry, and conversely, inhibit pre-replication origin loading and exit from mitosis. Cyclin synthesis and degradation, Cdk phosphorylation and Cdk …
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Regulation mechanisms of Drosophila retinoblastoma protein stability and repression activity
… I investigated the effects of phosphorylation by Cyclin-Cdk complexes. I show that protein stability and activity governed by the IE are cleanly separable, possibly by coordinated interactions with E2F transcription factors and E3 ubiquitin ligases. A highly conserved lysine residue K774 in the …
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Determination Of The Essential Functions Of A Conserved Cyclin, Cyclin Y, In Drosophila
… encodes a member of a highly conserved family of cyclins, the Y type cyclins, which have not been functionally characterized in any organism. Here I report the generation and phenotypic characterization of a null mutant of <i>CG14939</i>, which we rename <i>Cyclin Y</i> (<i>CycY</i>). I show that …
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Regulation of Anaphase Promoting Complex Coactivators
… G1; inhibitory phosphorylation of Cdh1 by CDK and Polo kinase may allow accumulation of Cdh1 targets in the subsequent cell cycle. I demonstrate lethality of exact endogenous gene replacement of CDH1 with the CDK-unphosphorylatable CDH1-m11 allele; neither polo kinase sites nor polo …
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Loss of Chk1 Function and Exogenous Expression of Cyclin A1/Cdk2 Results in Apoptosis after the MBT in Early Xenopus laevis Embryos
… the role of two cell cycle components, Chk1 and cyclin A1/Cdk2, during apoptosis was studied during early development of Xenopus embryos. Chk1 is a serine/threonine kinase that inhibits the activity of cyclin-dependent kinases (Cdks) in response to unreplicated DNA. In the pre-MBT embryo, Chk1 is …
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Regulation of Global Transcription Dynamics During Cell Division and Root Development
… that do not express either S-phase or mitotic cyclins we quantify the relative contributions of cyclin-CDK complexes and transcriptional regulatory networks in the regulation the cell cell expression program. We show that CDKs are not the sole regulators of periodic transcription as contrary to …
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The B55α/PP2A Holoenzyme in Cell Cycle Exit, Maturation/Differentiation, and Cancer
… in part by an equilibrium between inducible CDKs and the serine/threonine protein phosphatase PP2A. Protein Phosphatase 2A (PP2A), is a serine/threonine phosphatase that functions as as a collection of trimeric holoenzymes. The trimeric PP2A holoenzyme is composed of the "A" scaffolding …
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Characterization of the molecular mechanisms underlying retinoic acid induced growth inhibition in MCF-7 human breast cancer cells.
… (pRb). It also resulted in a down regulation of cyclin dependent kinase 2 (cdk2) and cyclin D1 gene expression and a profound down regulation of cdk2 activity. In contrast, no changes were seen in cdk4 expression or activity. We have further demonstrated that RA did not cause a change in the …
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The Dynamics of the Unreplicated DNA Checkpoint in Xenopus laevis Embryos and Extracts
… pathways cause cell cycle arrest by inhibiting cyclin-dependent kinases (Cdks). In Xenopus laevis, early embryonic development consists of twelve rapid cleavage cycles between DNA replication (S) and mitosis (M) without checkpoints or gap phases. However, checkpoints are engaged in Xenopus once …
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Regulation of the Anaphase-Promoting Complex Examined at the Single Cell Level
… cycle transitions are driven by oscillations of cyclin-cyclin dependent kinase (CDK) activity and associated cyclin degradation, mediated by ubiquitylation by the anaphase-promoting complex (APC). In this work, I analyzed the regulation of the APC by its activator Cdh1 in budding yeast in single …
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Molecular Mechanisms of Growth Inhibition Mediated by Retinoic Acid in Human Pancreatic Cancer Cells
… cycle, as well as in cellular concentrations of cyclins and their associated cyclin-dependent kinase (CDK) sub-units and CDK inhibitors (CDKI) such as p21, p27 and p16. Previously published studies have shown minimal effects of natural retinoids in inducing such changes in pancreatic cancer cell …
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B cell signalling in mechanisms of central and peripheral tolerance
… has now been implicated in the maintenance of cycling ERK activation observed in spontaneously proliferating WEHI-231 B cells. BCR signalling was found to reduce the levels of active Rap, hence providing a mechanism for the BCR-mediated uncoupling of the Ras-independent, MEK-dependent ERK …