Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"cyan fluorescent protein"”.
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Design and synthesis of probes for detection of protein-protein interaction and RNA localization
… hydrazide system for detecting the formation of cyan fluorescent protein and NF-kappaB p50 dimers was assessed. A series of benzophenone-based probes were synthesized and tested for photocrosslinking activity to investigate the efficiency of photocrosslinking in these systems. Three series of …
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Methodology development for imaging histone modifications and for site-specific protein labeling in vitro and on the surface of living cells
… cells was developed using genetically encoded fluorescent reporters. These reporters were constructed for sensing histone phosphorylation and methylation by fluorescence resonance energy transfer (FRET). These reporters are four-part chimeric proteins with the domains from N- to C-terminus as …
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Rapid Rac GTP-ASE activation in live cells by mechanical stress is independent of Src
… guanosine triphosphatase, was quantified using a fluorescent resonance energy transfer (FRET) method that measures changes in Rac activity in response to mechanical stresses by quantifying intensity ratios of ECFP (enhanced cyan fluorescent protein as a donor) and YPet (a variant yellow …
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Leveraging Genomic Information for Safer Gene Therapy
… novel insulator sequences using two expression protein marker system, cyan fluorescent protein (CFP) for validation of sequence integration and green fluorescent protein (GFP) for measurement of strength of insulator sequence. Our findings showed that CTCF and RAD21 occupancy as the major but …
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Correlating antisense RNA performance with thermodynamic calculations
… mRNA hybridization were computed based on the fluorescent protein reporter genes. Three different fluorescent proteins (i) green fluorescent protein (GFP), (ii) cyan fluorescent protein (CFP) and (iii) yellow fluorescent protein (YFP) were used as reporters. Each fluorescent protein was cloned …
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Anti-GD1a antibody targeted disruption of the node of Ranvier in a mouse model of acute motor axonal neuropathy
… both to over-express GD1a and to express cyan fluorescent protein (CFP) in the cytoplasm of axons. Endogenous fluorescence allowed identification of intramuscular nerve bundles and their terminal branches, which were categorized depending on bundle size. Within these categories, IgG and …
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Thermodynamics of λ-PCR Primer Design and Effective Ribosome Binding Sites
… in the primer designs to test heterologous protein expression of the cyan fluorescent reporter with different RBS strengths. These RBS sequences were designed with an online tool, the RBS Calculator. A chimeric primer design procedure for λ-PCR was developed and shown to effectively create …
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Examining the Role of Phospholamban Phosphorylation on Interaction with SERCA Using Fluorescence Microscopy
… many forms of heart failure (HF), making these proteins potential targets for the treatment of HF. Both proteins have been extensively studied in vitro, where their basic structure and function were determined, and in animal models, where their role in disease was examined. However, key …
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Evaluating luteinizing hormone receptor signaling using the cyclic AMP reporter ICUE3
… hormone (LH) receptor is a member of the G protein-coupled receptor family and the subfamily of glycoprotein hormone receptors. The LH receptor plays a vital role in normal development and in the function of the gonads. The LH receptor is expressed in interstitial cells, thecal cells, and …
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Analysis of nerve terminal dysfunction in autoimmune neuropathy
… to produce a mouse line that expresses green-fluorescent protein (GFP) in TSCs, and cyan-fluorescent protein (CFP) in axons (CK mouse). Live imaging techniques were used to study the acute and chronic effects of EG1 mAb mediated, complement dependent glial injury in this system. It is shown …
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Rotational motion and organization studies of cell membrane proteins
… Plasma membrane receptors are integral membrane proteins with diverse structures and functions which bind specific ligands to trigger cellular responses. Due to compartmentalization of the plasma membrane and the formation of membrane microdomains, receptors are distributed non-homogeneously in …
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Exploring a Nuclear Insulin Receptor Signalling Pathway in Drosophila Neural Stem Cells
… is studied via a variety of *in vivo* methods. Cyan fluorescent protein (CFP)‐tagged InR was expressed with the GAL4 system to examine its subcellular localization. InR‐CFP is present in the nucleus of *Drosophila* NSCs throughout CNS development. This localization pattern is not upheld in NSC …