Global ETD Search
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Showing 1 to 20 of 24 for “"curli"”.
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Formation and Pathogenicity of Cytotoxic Curli Intermediates
… component of the biofilm and has identified that curli and extracellular DNA (eDNA) complex irreversibly within the biofilms of Salmonella enterica serovar Typhimurium and Escherichia coli. Here, we investigate the formation and pathogenicity of cytotoxic curli intermediates previously …
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REGULATION AND EXPRESSION OF AMYLOID CURLI IN SALMONELLA INFECTION
… is a thick extracellular matrix composed of curli, cellulose, BapA, and extracellular DNA. Curli are functional amyloids with a rich β-sheet structure strikingly similar to pathological and immunomodulatory human amyloids such as β-amyloid, α-synuclein, and serum amyloid A. It is often …
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Curli Production and ERIC-PCR Fingerprinting of Escherichia coli from Clinical Cases of Bovine Mastitis
… clinical severity, and impairing recovery. Curli are adhesive surface structures produced by some E. coli and Salmonella strains that bind a number of host proteins and have recently been found to play a role in the pathogenesis of bacterial sepsis. Sixty-one E. coli isolates from 36 …
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H-NS Counter-silencing as a Primary Regulatory Mechanism of Curli Gene Expression in Salmonella Typhimurium
… genes, such as the csg regulon required for curli synthesis, and silence their expression. While previous studies have implicated a role of the alternative sigma factor σS in directly disrupting H-NS silencing, our system reveals that σS plays a more indirect role, acting through …
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THE ROLE OF BACTERIAL AMYLOID FIBRILS IN ESCHERICHIA COLI COMPLEMENT RESISTANCE
… complement system. The bacterial amyloid fibril, curli, functions in bacterial adherence and the formation of biofilm. Curli-producing parental and curli-deficient mutant E. coli was compared in its survival to human complement, using in vitro serum sensitivity assays. Results showed an increase …
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Contributions of type 17 immunity and type I interferon signaling to Salmonella-induced autoimmunity
… Salmonella biofilm-associated amyloid protein, curli, is a potent inducer of systemic inflammation and autoimmune responses. Curli induces immune responses via various receptors, on the cell surface through toll-like receptor (TLR) 2/1 and CD14, and in the cytosol through nucleotide-binding …
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The Role of the Extracellular Matrix components of Salmonella enterica serovar Typhimurium Biofilms in Biofilm Formation and Architecture via in vitro analysis
… Typhimurium (STm) biofilms is composed of curli, cellulose, BapA, O-antigen capsule, colanic acid and extracellular DNA (eDNA) and protects the resident bacteria from diverse environmental stresses such as antibiotics and the host immune response. Studies have shown that curli, which …
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The Role of Bacterial Amyloids In Regulating Gastrointestinal Homeostasis
… as essential adhesins during biofilm formation. Curli fibers expressed by Enterobacteriaceae family members including E. coli and S. Typhimurium are the most studied amyloids to date. Curli-like fibers are also produced by members of the predominant phyla found in the host gastrointestinal …
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Mechanisms Associated with Attachment of Escherichia coli O157:H7 to Lettuce Surfaces
… expressing various extracellular proteins (curli, O157-antigen, and intimin) known to influence attachment of E. coli to intestinal cells were evaluated for their physicochemical properties and ability to adhere to cut edge and whole leaf lettuce. Escherichia coli O157:H7 strains included: …
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INVESTIGATION OF ANTIBIOFILM AND ANTIVIRULENCE ACTIVITY OF 5-FLUOROCYTOSINE IN ESCHERICHIA COLI
… all three fluoropyrimidines caused inhibition of curli production and general reduction of biofilm adhesion in dose-dependent manner in MG1655. This result was further confirmed by the observation that fluoropyrimidines can downregulate transcription of the curli structural gene csgA, suggesting …
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THE SUBTLE BIOFILM REGULATION IN ESCHERICHIA COLI: CSGD AND THE YDDV-DOS OPERON
… to environmental signals and on regulation of curli fibers, cellulose and poly-N-acetylglucosamine (PNAG), the most important biofilm determinants in E. coli. E. coli is an Enterobacterium, normally living inside the mammalian gut, at temperature of 37° C and in relatively nutrient-rich …
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The Dendritic Cell Response to Exogenous and Endogenous Danger Signals
… of bacterial biofilms, the amyloid-like protein “curli”, irreversibly forms fibers with bacterial or eukaryotic DNA during biofilm formation. This interaction accelerates amyloid polymerization and creates potent immunogenic complexes that activate immune cells, including dendritic cells, to …
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Novel techniques for the in situ detection of bacteria on salad leaf surfaces
… serovar Typhimurium mutants defective in curli fimbriae production were used to assess the role of curli in attachment to abiotic surfaces and leaves. Viability determination of potential viable but non-culturable (VNC), sub-lethally stressed cells was performed to assess the effect of …
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Untersuchungen zum Einfluss der ATP-abhängigen Protease ClpXP auf die Regulation und Expression Virulenz-assoziierter Gene des uropathogenen E. coli Stammes 536
… kodieren. Zudem exprimiert der Stamm Curli-Fimbrien, eine Flagelle vom Serotyp H31 und ist aufgrund seines glatten Lipopolysaccharid Phänotyps (O6 Antigen) serumresistent. Ziel dieser Arbeit war es, den Einfluss der Protease ClpXP auf die Regulation und Expression Virulenz-assoziierter …
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The structure and function of TasA in the <i>Bacillus subtilis</i> biofilm matrix
… including the <i>Enterobacteriacae</i> Curli, <i>Staphylococcus aureus</i> Bap, <i>Pseudomonas</i> Fap and <i>Bacillus subtilis</i> TasA protein fibres that give structure to the respective biofilms. However, the categorisation of ‘amyloid-like’ to fibre-forming proteins of the biofilm …
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Shigella Boydii 18: Characterization and Biofilm Formation
… ATCC transformants produced copious amounts of curli as revealed in scanning electron micrographs. Fit(TM) produce wash and water yielded reductions in populations of S. boydii transformants on parsley similar to those observed with the wild type.
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A combinatorial approach for improved target prediction, validation, and characterization of small RNAs in Escherichia coli
… ABC transporter and the master regulator of curli biosynthesis, respectively, and activate cfa, which is involved in membrane reconstruction. We found that RydC represses pheA, which encodes a chorismate mutase and is involved in phenylalanine biosynthesis, and activates trpE, encoding an …
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Post-Transcriptional Regulation of Virulence Genes by GlmY and GlmZ in Enterohemorrhagic E. Coli
… strains. Aside from the LEE, GlmZ also regulates curli which are used to facilitate bacteria attachment to host cells. These data show that GlmZ has been co-opted into being an important regulator of virulence genes in EHEC.
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Phenotypic, Genotypic, and Pathogenic Evaluation of E. coli ST131 Clinical Isolates
… epithelial cell adhesion and invasion, and curli production. Twenty UPEC clinical isolates of E. coli ST131 and non-ST131 were used for both in vivo and in vitro studies. Six mice per isolate were inoculated via urethral catheterization. CFUs were determined from bladder and kidneys. In …
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Phenotypic and Genotypic Characteristics for Escherichia coli strains responsible for bacterial bloom events in Australia
… for a free-living lifestyle such as flagella and curli were disrupted in B1-001. In Colilert-18, B1-001 was heavily outcompeted by the two phylogroup A bloom strains from the east coast, explaining why B1-001 was not detected in recent bloom events assessed using Colilert-18. The current study …
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