Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 201 for “"culture media"”.
-
Chromotography for Amino Acid Analysis of Tissue Culture Media
The first phase of the problem was to become acquainted with paper chromatography and to find the technique which would be most suitable for subsequent experiments. A modification of Redfield's (29) method of two-dimensional paper chromatography was used. Slight changes were made with his method …
-
Integrated Microfluidic Culture Media Oxygenator for Organ-on-a-Chip Applications
… systems (MPS) are in vitro platforms for the culture of human cells in a manner that closely mimics the in vivo physiological microenvironment. Oxygen is a key element for maintaining cell viability and function in MPS devices. Thus, this thesis presents the design, fabrication, and testing of …
-
Ion and Amino Transport in Rabbit Kidney Tissue in Tissue Culture Media
… that occur when cells are incubated in tissue culture media as opposed to changes that occur when they are incubated in natural media, i.e., serum.
-
Maturation and Culture Media Effects on In Vitro Bovine Embryo Developmental Competence
… aim of this thesis was to alter maturation and culture media to improve the developmental competence of <em>in vitro </em>bovine embryos. This thesis is comprised of three experiments and one proof of concept study. Each experiment followed the same general layout: oocyte aspiration from Jersey …
-
Immunoassay sensitivity and kinetic enhancement in cell culture media using electrokinetic preconcentration
The microfluidic cell culture enables the study of cell signaling in previously impossible or impractical ways by allowing the precise spatial and temporal control of the microenvironment to better mimic in vivo conditions. Microfluidic techniques allow the creation of exact culture geometries, …
-
Diffusion and reactions of nitric oxide, oxygen, and superoxide in cells and culture media
… profiles of NO and related species in cell cultures and tissues. Toward that end, the diffusion and reactions of NO, 02, and 02- were studied by mathematical modeling and by experimental work with macrophage cultures. The results obtained will be useful in designing NO toxicity experiments …
-
Optimizing embryo culture conditions and spent culture media analysis as predictors of embryo quality and pregnancy
… aim of this thesis is first, to evaluate various culture conditions to improve embryo development, and secondly, to analyze spent culture media for any biomarkers that may be predictive of embryo health. Single-step and sequential culture media were compared in both Planer and EmbryoScope™ …
-
The Fate of Biological Agents on Cell Culture Media and the Final Impact on Cell-Based Foods
Cell-based foods are a novel category of food which has garnered significant interest and investment in recent years. Cell-based foods are covered by legal statutes, and the FDA and USDA are among the regulators who have oversight. A science-backed approach is therefore needed to determine the …
-
Supplementing Bovine Embryo Culture Media to Improve the Production and Quality of In Vitro Produced Bovine Embryos
… of zinc sulfate and a bovine embryonic stem cell culture media (TeSR) for improving bovine embryo development in vitro. Zinc sulfate increased day 8 blastocyst total and ICM cell number. Therefore, zinc sulfate appears to improve blastocyst quality. The TeSR medium improved embryo development …
-
Use of Locally available materials in the development of culture media for the production of Cheese starter cultures in Zambia
… of cheese processing studied.Lyophilized stock cultures obtained from KCF,ZA de Buxiers BP10 of France and a mother culture from DPBZ were used as principal organisms to test the efficacy and sensitivity of all media used in this study. Eight complex broth media were constructed in the …
-
Use of Locally available materials in the development of culture media for the production of Cheese starter cultures in Zambia
… of cheese processing studied.Lyophilized stock cultures obtained from KCF,ZA de Buxiers BP10 of France and a mother culture from DPBZ were used as principal organisms to test the efficacy and sensitivity of all media used in this study. Eight complex broth media were constructed in the …
-
Evaluation of the effect of growth conditions on lipid production by Papiliotrema laurentii UFV-1 in culture media containing xylose
… superior values can be achieved depending on the culture conditions. Papiliotrema laurentii UFV-1, contrary to the oleaginous yeast model Yarrowia lipolytica, is capable of growing and accumulating lipids from xylose, the main sugar found in hemicellulose. Therefore, this work aimed to evaluate …
-
Effect of different culture media and incubation methods on culturing murine embryos in vitro using a semen straw as an alternative receptacle
… of the study was to fertilize mouse oocytes and culture the resulting zygotes in bi-gas incubator and in a goat vagina and compare the in vitro embryo development in TCM-199 and Ham's F10 culture media until the blastocyst formation. The spermatozoa and oocytes of an F1 generation (Balb C x C57) …
-
Investigating a novel in vitro embryo culture system – The Walking Egg Affordable Assisted Reproductive Technology
… is the development of a low-cost embryo culture method by The Walking Egg foundation. This method utilizes a standard chemical reaction and simple equipment to equilibrate culture media pH and to regulate temperature; both aspects were investigated in this study. An exploration into the …
-
Identification, characterization and amino terminal sequencing of high molecular weight soluble antigens of the human malaria parasite, Plasmodium falciparum
… supernatant fractions obtained from in vitro culture media or partially purified fractions of culture media have been shown to protect them from malaria. Protective immunity is known to occur naturally in afflicted adolescent and adult individuals only after long-term exposure to malaria.
-
Developing a labview based thermally stimulated current (tsc) controller to measure residual charge in electrospinning
… and decay of residual charge during cell culture media is still unknown. In an attempt to clarify the presence or absence of residual charge during cell culture, a LabVIEW based Thermally Stimulated Current controller is designed. The LabVIEW controller encapsulates the temperature and the …
-
Comparison of two different media and assisted hatching techniques on the embryo hatching rate using the mouse as a model
The use of in vitro culture media and assisted hatching techniques remain a challenging obstacle to hatching of blastocyst-stage embryos. Mechanical, chemical, enzymatic thinning and laser assisted techniques have been used previously, but there is still a lack of information on its application and …
-
Comportamento in vitro de Chalara paradoxa sob diferentes condições de cultivo
… conditions of this pathogen involving different culture conditions, such as culture media, temperatures and luminosities, which are key factors in determining the behavior of the pathogen. Therefore, the objective of this work was to evaluate the in vitro behavior of C. paradoxa in different …
-
Designing and constructing a prototype standalone bioreactor using 3D printing and finite element analysis : a tool to define osteogenic differentiation in a 3D mechanical environment
… chemical, and mechanical properties to those cultured in vivo. In vivo tissues are embedded into complex environments, communicating both with nearby zones and with the whole organism, which determines the tissue-specific function. Special instruments called bioreactors are used to mimic these …
Page 1 of 11