Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 257 for “"culture conditions"”.
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Effects of three-dimensional culture conditions on skeletal muscle myoblasts
Effects of Three-dimensional Culture Conditions on Skeletal Muscle Myoblasts\r\n\r\nPublication No._____________\r\n\r\n\r\nMichele Lynn Marquette, Ph.D.\r\nThe University of Texas Medical Branch, 2007\r\n\r\nSupervisor: Marguerite A. Sognier\r\n\r\nThe objectives of this research were to: 1) …
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The effects of culture conditions on cell metabolism and function
… profoundly affects the capacity of cell culture to model physiological and pathophysiological states. Central to the modern optimisation of cell culture is the development of physiologic media, which highlight the major impact that specific nutrients can have on a variety of cellular …
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Factors affecting bone cell growth and differentiation under differing culture conditions
… in osteoblast differentiation in 2D versus 3D cultures remain unclear. In this study, rat marrow stromal cells (MSCs) were grown both on plastic and in 3D polymer scaffolds and their differentiation into osteoblasts studied. MSCs cultured in a synthetic 3D matrix differentiate faster into …
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Bioenergetics and growth of white steenbras, Lithognathus lithognathus, under culture conditions
… steenbras, Lithognathus lithognathus, under culture conditions were determined to assess its suitability for mariculture. Fish were captured by seine netting, from nearby estuaries and transported to the laboratory where they were successfully kept for the duration of the experimental period. …
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Effects of culture conditions on development of early murine and bovine embryos
… development in vitro. Bovine morulae also were cultured to examine the effect of agar embedding and the environment of immature mouse uteri on embryo development. Mouse embryos were cultured for up to 96 h in M2 medium or M2 supplemented with CLBl 107. One-and two-cell embryos did not develop …
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The effect of hypoxia and 3D culture conditions on heterogeneous ovarian cancer spheroids
… metastasis potential of ovarian cancer. SVF co-cultured spheroids further increased the glycolytic shift of the heterogeneous of ovarian cancer spheroids, induced the aggressive phenotype by elevating the corresponding protein markers. Decreasing the glycolytic shift and suppression of the …
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Optimization of culture conditions and extraction method for phycocyanin production from a hypersaline cyanobacterium
Submitted in fulfilment of the requirements of the degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2014.
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Fundamental differentiation and growth characterization of murine embryonic stem cells in varied culture conditions
… of ES cells was also conducted with different culture conditions of reduced serum and glucose concentrations both in the presence and absence of leukemia inhibitory factor (LIF) which prevents spontaneous differentiation, as well as at varied LIF concentrations and seeding densities. In …
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Optimization of culture conditions for equine bone marrow mesenchymal stem cells and their differentiation into osteoblasts
<p>The horse industry is a multibillion dollar industry where the health of the animals is of great focus for horse owners. Bone fractures are an aspect of equine health failure that is continually catastrophic and often fatal. In addition to discussing fracture, this review will outline the …
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Optimizing embryo culture conditions and spent culture media analysis as predictors of embryo quality and pregnancy
… aim of this thesis is first, to evaluate various culture conditions to improve embryo development, and secondly, to analyze spent culture media for any biomarkers that may be predictive of embryo health. Single-step and sequential culture media were compared in both Planer and EmbryoScope™ …
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An investigation of protein variation in Ulva lactuca and Ulva rigida under high nitrogen culture conditions
… the protein content of two macroalgae under culture conditions. Ulva lactuca and U rigida were grown in saturating NH₄⁺ - N (1 mM) and PO₄³⁻P (70 μM ) concentrations. Of the two, protein levels of U. lactuca showed the greatest increase over time. Natural populations had a content of 3.7 % of …
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Optimization of Culture Conditions for in Vitro Fertilization of the Microphallid Trematodes Gynaecotyla Adunca and Microphallus Turgidus
… of this protocol on the production of eggs in culture. <em>Microphallus turgidus</em> has been successfully cultured in vitro (Pung, et al., 2011) and was used in this study as a control. Metacercarial cysts of<em> G. adunca</em>, were obtained from the green glands of fiddler crabs, <em>Uca …
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Cell-to-cell variability and culture conditions during self-renewal reversibly affect subsequent differentiation of mouse embryonic stem cells
… despite efforts to control the differentiation conditions and the use of a clonal starting population. In addition, this distribution may be affected by the cell microenvironment during self-renewal. Prior studies on self-renewal culture of ESC, however, focused on long term proliferation and …
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Effect of culture conditions, donor source, and injection site on in vitro development of deoxyribonucleic acid microinjected porcine zygotes
… of experiments were used to evaluate three culture media and two incubation temperatures for their ability to support development of DNA microinjected porcine zygotes. Development in vitro was compared between embryos collected from postpubertal and prepubertal donors and between embryos …
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The effects of temperature, water quality and culture conditions on the immunology, hematology, and blood chemistry of hybrid striped bass
… and serum chemistry changes under different culture conditions. The kinetics of the humoral immune response was determined for sunshine bass acclimated to 10, 18, 24, 29°C, and to elevated ammonia (0.15 mg/L NH3) and elevated nitrate (200 mg/L). These conditions are frequently encountered in …
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Studies on the novel detection and destruction of the enteroparasite Giardia lamblia and other veterinary problematic microorganisms examined under varying culture conditions
… Ireland by using a combined in vitro HCT-8 cell culture-quantitative PCR assay for evaluating the efficacy of using pulsed UV light for treating G. lamblia parasites. This study aims to provide a source of data into novel disinfection mechanisms such as Pulsed UV light technologies as a potential …
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Performance characterization of Erwin, Shasta, and Kamloops strains of rainbow trout under culture conditions at White Sulphur Springs National Fish Hatchery, West Virginia
… River Kamloops) were collected to guide fish culture operations at White Sulphur Springs National Fish Hatchery (NFH). The performance of the strains was evaluated in terms of survival, growth, disease resistance, reproductive performance (number of ripe and culled females, spawning time, …
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Hypoxia-induced responses of porcine pulmonary veins.
… pulmonary veno-occlusive disease. Blood vessel culture can be a powerful tool to enable assessment of the impact of environmental factors on vessel function and as a disease model. However culture conditions alone affect vessel contractility; the effect of culture conditions on PV function …
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Uncovering Constraints On Organoid Morphologies
… an organoid’s morphology by manip- ulating the culture conditions, the morphology of an organoid often resembles that of its original organ, suggesting that organoid morphologies are governed by a set of tissue-specific constraints. Here, we establish a framework to identify constraints on an …
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The Effects of Herbicides on Three Soil Inhabiting Blue Green Algal Species
… obtained from soil samples and grown under culture conditions in the laboratory.
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