Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"ctDNA"”.
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Monitoring trace levels of ctDNA using integration of variant reads
In patients with early-stage cancer, ctDNA detection rates can be low due to the presence of few or no copies of any individual mutation in each sample. Sensitivity may be increased by collecting larger plasma volumes, but this is not feasible in practice. Although cancers typically have thousands …
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Assessment of the clinical validity of ctDNA Analysis for melanoma management
… are urgently required. Circulating tumour DNA (ctDNA) analysis has emerged as a potential “liquid biopsy” for melanoma. Plasma-derived ctDNA are short DNA fragments released into the bloodstream by apoptotic tumour cells. Studies have shown that ctDNA levels in blood correlate with tumour burden …
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Establishing the feasibility of using ctDNA for molecular profiling in DLBCL
… pipeline for use on DLBCL FFPE biopsy and plasma ctDNA samples. This was conducted in the context of a prospective observational clinical trial, DIRECT. After establishing the variant calling pipeline, the next aim of the project was to compare genotyping profiles from baseline biopsy and plasma …
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Development and evaluation of methodologies for analysis of CTC and ctDNA in patients with ovarian carcinoma
… tumour cells (CTC) and circulating tumour DNA (ctDNA) represent a new generation of biomarkers that can be used for predicting therapy responsiveness and longitudinal monitoring of OC patients undergoing therapy. This thesis describes a series of investigations in OC which include, a …
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Investigating the Mechanisms and Kinetics of Circulating Tumour DNA Release to Improve its Clinical Utility
… treatment regimens. Circulating tumour DNA (ctDNA) has sparked tremendous interest in the last decade as a non-invasive method to monitor dynamic changes in treatment response. However, the mechanisms that dictate ctDNA release kinetics have not been thoroughly investigated, hampering the …
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Detection of trace levels of circulating tumour DNA in early stage non-small cell lung cancer
… using analytes such as circulating tumour DNA (ctDNA), can detect and quantify cancer in a minimally invasive way and can provide information on tumour heterogeneity. Current limitations in the liquid biopsy field are centred around the general sensitivity of the present assays and the input and …
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Improving earlier non-invasive diagnosis of high-grade serous ovarian cancer
… developing a diagnostic circulating tumour DNA (ctDNA) biomarker for high grade serous ovarian cancer (HGSOC), was to investigate low-cost high-throughput next generation sequencing assays in plasma samples collected from women with newly diagnosed OC. The secondary aim was to apply these methods …
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Detection and monitoring of treatment resistance in metastatic cancer through non-invasive DNA methylation-based biomarkers
… on empowering plasma circulating tumor DNA (ctDNA) analysis, as a non-invasive approach for precision oncology, with applications ranging from early detection and minimal residual disease (MRD) assessment to dynamic monitoring of treatment response and molecular profiling in advanced disease. …
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Circulating tumour DNA: A non-invasive biomarker for melanoma
… of mutant specific circulating tumour DNA (ctDNA) is an emerging tool for detection of residual disease and for prognosis and monitoring of different cancers (Bettegowda et al., 2014; Dawson et al., 2013; Gray et al., 2015; Spindler et al., 2012). There is however, limited use of ctDNA for …
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Multi-Modality Plasma-Based Detection of Minimal Residual Disease in Triple-Negative Breast Cancer
… I focused on detecting circulating tumor DNA (ctDNA) from TNBC patients after neoadjuvant chemotherapy and surgery. First, patient-specific somatic mutations were identified by sequencing primary tumors. From these data, 82% of the patients had at least one TP53 mutation, followed by 16% of the …
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Non-Invasive cancer detection: computational applications in liquid biopsy and radiomics
… comprehensively analyzing circulating tumor DNA (ctDNA), particularly at low ctDNA fractions, and introduces novel tools and strategies to enhance the sensitivity and specificity of ctDNA analysis. The first part of the thesis focuses on the development of Synggen, a tool for generating synthetic …
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Exploring the effect of peri-operative inflammation on systemic and local breast cancer biomarkers
… and influence circulating tumour DNA (ctDNA)release which may predict clinical and oncological outcomes. Aims 1. To understand the relationship between peri-operative systemic inflammation and breast tumour characteristics, surgical intervention and 30-day morbidity in non-metastatic …
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Targeted next generation sequencing to improve solid tumour diagnosis using tissue-derived and cell-free DNA
… from tissue or circulating tumour-derived DNA (ctDNA) from plasma. ctDNA is highly fragmented DNA released into the bloodstream from tumour tissue and is a common analyte for liquid biopsy in both the research and clinical setting. There is little literature surrounding the use of targeted NGS …
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Amplifying signals in the tumor microenvironment for drug development and diagnostics
… genomic profiling of circulating tumor DNA (ctDNA) molecules in liquid biopsies such as blood. Despite the promise offered by these innovative therapies and monitoring tools, their broad integration into clinical practice faces important challenges. Widespread adoption of emerging therapies …
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Decoding the RNA cargo of plasma-derived extracellular vesicles in metastatic castration-resistant prostate cancer
… a broad spectrum of circulating tumor DNA (ctDNA) levels, as opposed to high ctDNA patients only, it offers a more inclusive perspective on prognostic indicators. Integrating EV transcriptomics, ctDNA estimation, physical measurements of EVs, nucleic acid quantifications, blood counts and …
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Resolving the evolution of Oesophageal Adenocarcinoma using spatial and temporal sampling
… tissue is through circulating tumour DNA (ctDNA). Liquid biopsy sampling also has been proven to be highly prognostic in multiple cancer types and recent advances in ctDNA detection have now provided a potential avenue for this sampling approach to utilised in OAC. Hypothesis Deciphering …
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Characterising DNA methylation in tissue and liquid samples from patients with renal tumours
… to ccRCC tumorigenesis. Circulating tumour DNA (ctDNA) represents a promising target for non-invasive liquid biopsy in both diagnostic and prognostic applications. Mutational analyses of ctDNA have produced disappointing detection rates in ccRCC, possibly hampered by low ctDNA levels and high …
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Molecular Analysis of Breast Cancer Transcriptomes, Genomes, and Circulating Tumor DNA
… rearrangements present in circulating tumor DNA (ctDNA) to detect occult metastatic breast cancer was evaluated. In total, 14 eventual metastatic (EM) patients and 6 long-term disease free (DF) patients were investigated. We used whole-genome sequencing on the primary tumors to derive …
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Detection of AR gene rearrangements and regulation of AR variant-driven prostate cancer models
… (CTCs), and circulating cell-free tumor DNA (ctDNA). Additionally, we profile a technique we developed for detection of AR gene rearrangements from CTC DNA. Using this approach, we analyzed matched CTC DNA and ctDNA from a cohort of ten CRPC patients and found that genomic alterations in CTC …
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Exploration of cell-free DNA’s biological properties for better understanding and improved circulating tumour DNA detection
… from cancer cells, circulating tumour DNA (ctDNA), forms a subset of cfDNA. It can be challenging to detect ctDNA as it often constitutes only a minor fraction of the cfDNA. Recent studies suggest that understanding the biological properties of ctDNA can improve its detection in a …
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