Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"crystallins"”.
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Crystallins and avian phylogeny
Contains fulltext : mmubn000001_029086396.pdf (Publisher’s version ) (Open Access)
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Alkylation of rat lens crystallins with iodoacetamide
… protein total free-sulfhydryl of the crystallins had reacted after 1 hr at pH 8, 25°C. Alkylated α-, βL-' and y-crystallin fractions demonstrated no altered chromatographic behavior on Sephacryl S-200; only alkylated βH-crystallin was altered so that it co-chromatographed with …
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Insights into the structure and aggregation of lens crystallins and other aggregation-prone proteins
… to the long-lived alpha-, beta- and gamma-crystallins are associated with cataract and their study has provided insight into the molecular basis of this disease. In this thesis, alpha- and gamma-crystallin isoforms have been characterised under crowded and oxidative conditions, …
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Insights into the structure and aggregation of lens crystallins and other aggregation-prone proteins
… to the long-lived alpha-, beta- and gamma-crystallins are associated with cataract and their study has provided insight into the molecular basis of this disease. In this thesis, alpha- and gamma-crystallin isoforms have been characterised under crowded and oxidative conditions, …
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Stability, unfolding, and aggregation of the gamma D and gamma S human eye lens crystallins
… in mature lens fiber cells. The 0- and y-crystallins are thought to be primarily structural proteins while a-crystallin possess an additional chaperone activity. Aggregation of partially unfolded or covalently damaged forms of these proteins results in cataract, which is the leading cause …
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Functional, Biochemical and Molecular Analyses of the *Cold Stable Eye Lens Crystallins From the Antarctic Toothfish Dissostichus Mawsoni
… of the cDNAs encoding for the toothfish crystallins revealed a total of 21 isoforms (2 alphas, 6 betas, and 13 gammas). Phylogenetic analyses suggest that toothfish alpha and beta crystallins are orthologues of mammalian counterparts, while gammas do not. Further separation, partial …
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In vitro proteolysis of ovine lens crystallins by calpain II : A dissertation submitted in partial fulfilment of the requirements for the Degree of Bachelor of Science with Honours
… degradation of the major lens proteins, the crystallins, is an important component in many types of cataract.. At Lincoln University, a heritable cataract in sheep is being studied. The aim of this project was to set up an in vitro system to investigate the ovine crystallin degradation …
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Contributions of aromatic pairs of human Gamma-D-Crystallin to its folding, stability, aggregation, and interaction with human Alpha B-Crystallin
Two distinct groups of proteins, a-crystallins and [Beta][gamma]-crystallins, constitute 90% of the vertebrate eye lens soluble proteins. Long-term solubility and stability against unfolding and aggregation are essential properties of crystallins and crucial to the function of the lens. Aggregation …
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In vitro interactions of the small heat shock protein chaperone human [alpha]B-crystallin with its physiological substrates in the lens [gamma]-crystallins
… heat shock protein, is one of the ubiquitous crystallins in vertebrate lenses, along with the [beta][gamma]-crystallins. It is composed of two subunits (~ 20 kDa) aA- and [alpha]B-crystallin (aA- and [alpha]B-Crys), which form a hetero-oligomeric, polydisperse complex of ~ 800 kDa in the lens. …
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Antioxidant Intervention With manganese(Iii)-Salophen in the Selenite Cataract Model: Implications for Cataract Disease
… of GSSeSG. Extensive proteolysis of lens crystallins and loss of calcium homeostasis occur in cataractous lenses from a rat treated with sodium selenite. The visual protection with Mn(III)-salophen is accompanied by a partial loss of the calcium homeostasis, a net increase in sodium, and …
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Folding, stability and aggregation of the long-lived eye lens protein human gamma D crystallin
… in the human eye lens. H[gamma]D-Crys and other crystallins are found in insoluble protein inclusions associated with the eye disease cataract. H[gamma]D-Crys is expressed in utero and does not regenerate during life, thus necessitating high stability and solubility. Covalent damage, including …
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The characterization of human [gamma]D-crystallin mutants and their differential interactions with the lens chaperone [alpha]B-crystallin
… eye lens is composed of fiber cells packed with crystallins up to 450 mg/ml. Human yD-crystallin (HyD-Crys) is a monomeric, two-domain protein, predominantly localized to the lens nucleus. Both domains of this long-lived protein have double Greek key p-sheet folds with well-packed hydrophobic …
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Molecular Evolution of Visual System Genes in Fishes
… studies on two gene families, opsins and crystallins. Opsin proteins determine the wavelengths of light detected by the retina, while crystallin proteins contribute to lens transparency and refractory power. My studies focus on teleost fishes, because teleost visual ecology is …
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Potential roles for transforming growth factor-B during terminal differentiation of ocular lens cells /
… resulted in decreased synthesis of lens crystallins and no accumulation of a differentiation marker protein, phakinin. Measures of tritiated thymidine incorporation demonstrated that TGF-β stimulated, in a dose-dependent manner, cellular growth and spreading. The production of collagen …
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Mechanism of the efficient quenching of tryptophan fluorescence in human gamma crystallin
… to be H[gamma]siological relevant for the lens crystallins. Trp42 Steady-state and time-resolved fluorescence measurements combined with H[gamma]brid quantum Figure 1: The crystal structure of wild- mechanical-molecular mechanical (QM-MM) type H[gamma]D-Crys depicted in ribbon simulations …
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Mechanism of aggregation of oxidation-mimicking mutants of human [gamma]D-crystallin
… in the eye lens due to aggregation of the lens crystallins, misfolded because of various covalent modifications accrued over a lifetime. Such modifications include oxidation of Trp residues to more hydrophilic residues following exposure to ultraviolet light. Structural features of the …