Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"cryotolerance"”.
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Metabolic Regulation and Cryotolerance of In Vitro-Produced Holstein Embryos
… metabolic regulators on the viability and cryotolerance of <em>in vitro</em>-produced embryos. Pilot studies revealed that evaluating early (stage 6) versus late (stage 7) blastocysts did not affect the trend seen in results, nor did culturing embryos in continuous versus sequential media. …
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Effects of Dietary Conjugated Linoleic Acid Supplementation on Bovine Oocyte Lipid Metabolism, Lipid Composition and Embryo Cryotolerance
Variation in cryotolerance exists between embryos from different animal breed, species and management conditions. Reduced tolerance to chilling and cryotolerance of oocytes and embryos has been associated with greater cytoplasmic lipids (Kim et al., 2001; Seidel, 2006). Previous studies in the cow …
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Energy substrates, metabolic regulators, and lipid accumulation during culture of in vitro-produced bovine embryos
… PES to reduce lipid content resulted in improved cryotolerance, and FCS decreased cryotolerance compared to the control. Transfer of embryos treated with PES during in vitro culture did not affect pregnancy rates, conceptus losses, or fetal or post-natal development in calves born normally. The …
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Effects of Polydatin on In Vitro Bovine Embryo Developmental Competence, Metabolism, and Cryopreservation
… and altered metabolism which hinders their cryotolerance. Overall, the goals of this thesis were to improve oocyte and embryo culture with the antioxidant polydatin and to optimize slow freezing procedures. This thesis was designed as three experiments, and in each experiment, oocytes were …
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Investigating the Actions of Interleukin-6 Family Members on Post-Hatching Blastocyst Development in Cattle
… rate of frozen and thawed blastocysts, but no cryotolerance effects were observed in thawed blastocyst cell number or percentage of apoptotic cells within the blastocyst. A separate series of studies tested if supplementing IL6 and/or LIF altered the morphology of blastocysts during extended …
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Determining the role of nerve growth factor-beta in seminal plasma on bovine reproduction
… sire conception rate scores, and improves sperm cryotolerance. To test this hypothesis, accessory sex glands (ampulla, bulbourethral, prostate, vesicular glands) were harvested post-mortem from mature bulls and either frozen or formalin-fixed for Ngf gene expression and protein localization. …
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Lipid Quantification and Cryopreservation of In Vitro Produced Jersey Cattle Embryos
… and possibly improve embryo viability and cryotolerance of IVP embryos. The CT method and collapsing the blastocoele prior to cryopreservation resulted in higher blastocyst survival rate. Further studies including transfer of embryos to recipients are necessary to corroborate these results. …