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Showing 1 to 11 of 11 for “"cryoprotective agents"”.
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Novel Cryoprotective agents to improve the quality of cryopreserved mammalian cells
… of living cells, tissues and organs. The use of cryoprotective agents (CPAs) in combination with extremely low temperatures is mandatory for optimum biopreservation. CPAs (e.g., glycerol, trehalose, dimethyl sulphoxide (DMSO)), however, are relatively cytotoxic and compromise biopreserved cell …
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An investigation of the effect of cryoprotective agents on intermolecular disulfide formation
"A cryoprotective compound is one which prevents or decreases injury caused by freezing living material. Several of these agents are known. It is not known why or how cryoprotective agents protect. It is the purpose of this study to investigate one possible explanation, on the basis of the …
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Untersuchungen zur Kryokonservierung humaner Adipozyten zur mehrzeitigen autologen Transplantation
… of human cells, during cryopreservation, cryoprotective agents are a common group of chemical substances, in science. In this work we used hydroxyethylstarch, glycerol and a mixture of glycerol, dextran and polyvinylpyrrolidone. The vitality of the cells was measured before the …
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Fertility preservation of ovarian germ cells: the horse and deer models
… were used to determine the toxicity effect of cryoprotective agents on ovarian tissue pre- and post-cryopreservation. In study three, equine ovarian tissues were used to compare slow-freezing versus vitrification; and to determine the best cryoprotective agents for each cryopreservation method. …
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Controlled loading of cryoprotectants (CPAS) with cell volume constraints
… permeability of the cell membrane to water and cryoprotective agents. Due to the large size of oocytes that requires microscopy methods which could be difficult to implement, micro fluidic device is presented in this study based on Coulter-principle to simultaneously control and measure the …
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Development of an automated microfluidic system for the loading and unloading of cryoprotectants from mammalian oocytes
… platform for the introduction and removal of cryoprotective agents (CPAs) to and from the oocyte is described. By combining microfluidics, automation, and optical measurement systems, the proposed system aims to overcome the challenges inherent to manual vitrification, and improve upon other …
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Influence of mitochondrial membrane potential on the cryopreservation survival of hepatocytes
… the physical damage of freezing by adding cryoprotective agents and optimizing cooling rates. Membrane-permeable cryoprotectants, such as dimethyl sulfoxide, though widely used, can be extremely toxic to the cell. More natural, non-membrane-permeable cryoprotectants, inspired by …
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Universal robot for automated microinjection with applications in transgenesis and cryopreservation
… we demonstrated that robotic microinjection of cryoprotective agents in zebrafish embryos significantly improves vitrification rates and post-thaw survivability of cryopreserved embryos compared to manual microinjection, opening the door to large-scale cryo-banking of various aquatic species on …
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Regulation of Volume by Spermatozoa and Its Significance for Conservation Biology
… to high concentrations of various osmolytes and cryoprotective agents in isotonic medium to evaluate which compounds were able to penetrate the sperm plasma membrane. The osmotic responses of spermatozoa from strains of mice known to have spermatozoa of high (B6D2F1) and low (C57BL6) post-thaw …
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EFFECT OF CRYOPROTECTANT EQUILIBRATION ON TRANSMEMBRANE POTENTIAL OF BOVINE OOCYTES
… equilibration to and from high concentrations of cryoprotective agents. The focus of this study was to analyze the effect of large and rapid osmotically induced volume perturbation on the transmembrane potential of bovine oocytes. The aim of this study was to evaluate and examine the difference in …
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BACTERIAL PLAYERS IN CROP SYSTEMS: BENEFITS AND RISKS IN THE " ONE HEALTH" PERSPECTIVES.
… materials, and other different molecules, as cryoprotective agents to obtain a lyophilised PGP inoculum. Using as model bacterium Rhizobium sp. GR12 strain, interesting results were observed using a residual fraction obtained from the extraction of proteins from okara, a soy-milk by-product, …