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Showing 1 to 20 of 39 for “"cryoprotectant"”.
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Oh cryoprotectant, wherefore art thou cryoprotectant? Investigation of the permeation of common cryoprotectants into live rice callus cells by coherent Raman microscopy
… tissue samples are exposed to mixtures of cryoprotectants. Only a few cryoprotectant formulations have been used almost exclusively since their development in the early 1990s, including Plant Vitrification Solution 2 (PVS2) and Plant Vitrification Solution 3 (PVS3). Unfortunately, these …
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EFFECT OF CRYOPROTECTANT EQUILIBRATION ON TRANSMEMBRANE POTENTIAL OF BOVINE OOCYTES
A major physiological event during cryopreservation is equilibration to and from high concentrations of cryoprotective agents. The focus of this study was to analyze the effect of large and rapid osmotically induced volume perturbation on the transmembrane potential of bovine oocytes. The aim of …
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Concentration of Cryoprotectant in water-in-oil microdroplets for single cell vitrificaton
… is demonstrated to dynamically concentrate cryoprotectants within single cell-containing droplets. These droplets of sizes of about 30 micron diameter were concentrated to 3-4M from a starting concentration of IM in about 300s. The cells are tolerant to this concentration process and do not …
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An Agent-Based Model of Cryoprotectant Equilibration in Secondary Stage Preantral Ovarian Follicles
Young cancer patients have limited options for fertility treatment when facing gonadotoxic treatment. One promising fertility treatment for young cancer patients is the cryopreservation of immature ovarian follicles followed by maturation and subsequent reimplantation. However, preantral ovarian …
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The Effect of Arginine Vasotocin on Cryoprotectant Production during Freezing in the Pacific Chorus Frog, Pseudacris Regilla
… way of surviving during wintering months. Use of cryoprotectants is seen in all freeze tolerant species (glucose/glycerol). Synthesis and transportation mechanisms that regulate this process are not yet fully understood. Arginine vasotocin is a neurohypophyseal peptide known to have glycogenolytic …
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X-ray Computed Tomography as a method of cryoprotectant concentration measurement and ice detection: application to organ cryopreservation.
La criopreservación persigue la conservación de sistemas biológicos, como células, tejidos o pequeños organismos, a bajas temperaturas. Existen distintos procedimientos para llevar las muestras biológicas a temperaturas criogénicas, evitando la formación del principal causante de la muerte celular …
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Regulation of pyruvate kinase and glycerol-3-phosphate dehydrogenase in the freeze tolerant goldenrod gall fly: role in polyol cryoprotectant production
… The roles that these enzymes play in polyol cryoprotectant synthesis and metabolic regulation were investigated. A primary focus was the potential for reversible post-translational modifications (PTMs) to regulate enzyme activity. Several PTMs were analyzed for PK and G3PDH from control and …
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Dielectric Measurements for Organ Cryopreservation
… . Before cooling, the organ is perfused with a 'cryoprotectant', a solution which reduces freezing injury. Warming rates that can be achieved by thermal conduction cannot exceed about 1K/min without damaging the exterior of the organ. Experimental evidence from a range of cell and organ systems …
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The utilization of the prostacyclin analogue iloprost in the cryopreservation of stallion spermatozoa
… Most producers utilize glycerol as the primary cryoprotectant when freezing stallion semen, fully knowing that the cell undergoes significant damage during the cryopreservation process as well as from the exposure to the cryoprotectant itself. The current study evaluated the potential of the …
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New methods for cryopreserving rooster spermatozoa
… and osmotic intolerance. Glycerol is a common cryoprotectant used to protect cells during freezing, but its relatively large molecular weight and relatively low membrane permeability (compared to water) creates relatively long aniosmotic conditions when glycerol is added or removed. …
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Experimental Study of Advanced Cryopreservation Strategies for the Cardiovascular System: Superiority of Vitrification over Standard slow Freezing in Murine Vessels and Efficacy of Ultrafast Rewarming by High-Intensity focused Ultrasound (HIFU) in Cardiac Recovery from Deep Hypothermia (-6ºC)
… slow freezing, through the use of an optimized cryoprotectant cocktail and a controlled cryoprotectant loading-unloading approach that maintains high cellular viability and prevents structural damage. Moreover, the use of High-Intensity Focused Ultrasound (HIFU) in hearts enables the recovery of …
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Serial and Single Crystal Studies of Ligand Binding to Radiation Sensitive Haem Protein DHP-B
… hanging-drop vapour diffusion method, soaked in cryoprotectant and measured at synchrotron sources. Serial data was collected using microcrystals at SACLA, Japan and from DLS. The structures were solved using both CCP4i2 suite and Phenix. The structures displayed different binding modes and …
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The Rational Design and Use of Novel Small-Molecule Ice Recrystallization Inhibitors for the Cryopreservation of Hematopoietic Stem Cells and Red Blood Cells
… storage of cells. The most commonly used cryoprotectants are 10% dimethyl sulfoxide (DMSO) for hematopoietic stem cells (HSCs) and 40% glycerol for red blood cells (RBCs). DMSO fails to protect the functionality of HSCs after cryopreservation and therefore, up to 20% of HSC …
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Using Fluorescent Probes to Study the Location and Interaction of Disaccharides within Lipid Bilayers
… have focused on trehalose has a potential cryoprotectant. Cryopreservation involves storing hydrated cells or other materials at very low temperatures in a frozen state. Unfortunately, trehalose did not show effectiveness as a cryoprotecting agent. This may be due to trehalose being a …
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Cryopreservation of pancreatic islets
… cryopreservation protocols, which use the toxic cryoprotectant dimethyl sulphoxide (DMSO), achieve suboptimal cryosurvival and are not clinically used. The aim of this project was to develop an improved and clinically-relevant method for cryopreservation of islets. The project examined the …
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Regulation of the Nuclear Factor of Activated T Cell (NFAT) Family of Transcription Factors in the Freeze Tolerant Wood Frog, Rana sylvatica
… potentially linked with glucose production as a cryoprotectant). Anoxia activated NFATc4 in liver, leading to increased atrial natriuretic peptide levels. Neither freezing nor anoxia significantly affected NFATs in skeletal muscle. Overall, the study indicates that NFATs have a crucial role to …
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Through Vial Impedance Spectroscopy (TVIS): A novel process analytical technology for investigating the thawing of frozen cell suspensions.
… often relies on the use of chemical agents (cryoprotectants) which impact the (a) the rate of water transport through the cell membrane; (b) ice nucleation temperatures, and (c) the formation of both extracellular and intracellular ice. Despite the recognition of the importance of ice …
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MEMS Coulter counter for dynamic impedance measurement of time sensitive cells
… red blood cells and yeast cells were mixed with cryoprotectant agent and phosphate buffered solution. To quantitatively evaluate mixing efficiency, image processing technique was used to analyze color intensities variation of captured images of 2 dyed fluids mixed in the channel at different flow …
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The Engineering and Development of pH-Responsive Biopolymers for Drug Delivery Applications
… similar cryosurvival rate with trehalose as cryoprotectant compared the gold standard DMSO protocol. The success marked the importance of application-specific design and understanding of drug delivery systems.
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Cold-acclimation of the wood cockroach <i>Cryptocercus punctulatus</i> (Scudder) (Dictyoptera: Cryptocercidae)
… that ribitol, like other polyols acts as a cryoprotectant to stabilize protein structure, membrane integrity, and reduce osmotic fluctuations in freezing tissues.
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