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Showing 1 to 20 of 76 for “"cryopreserved"”.
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Mechanisms of cell damage and recovery in cryopreserved freshwater protists
… protein SH levels were also detected in cryopreserved algae. In <i>Haematococcus pluvialis</i> Flotow CCAP 34/8 a regulated antioxidant response and high carotenoid composition were considered to contribute to freeze-tolerance.<br/><br/>Novel techniques (encapsulation/two-step cooling) …
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Novel Cryoprotective agents to improve the quality of cryopreserved mammalian cells
Cryopreservation is a promising approach to long-term biopreservation of living cells, tissues and organs. The use of cryoprotective agents (CPAs) in combination with extremely low temperatures is mandatory for optimum biopreservation. CPAs (e.g., glycerol, trehalose, dimethyl sulphoxide (DMSO)), …
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Analysis of the Proteoglycan Content in Fresh and Cryopreserved Porcine Cardiovascular Tissues
… conduit tissue. Proteoglycans from fresh and cryopreserved porcine aorta conduit tissues were isolated by extraction with 4 M guanidine (Gdn) - hydrochloride (HCl) for 48 hours. Quantitative analysis of extracted proteoglycans revealed that the content of proteoglycan material from …
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Outflow Reconstruction using Cryopreserved Aortic Patch in Right Lobe Living Donor Liver Transplantation
… lobe graft, we reconstructed the new MHV using cryopreserved veins just like the MHV of the extended right lobe graft, and then made a venous pouch to form a common trunk between MHV (or new MHV) and RHV of the right lobe graft using a cryopreserved aortic patch. During graft implantation, …
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Factors affecting post-thaw quality of cryopreserved boar sperm and its effect on gilt fertility
In several species, cryopreservation of sperm has played a major role in genetic advancement as well as conservation of genetic resources. However, due to variability in post-thaw quality of sperm and decreased pregnancy rates and litter sizes, the swine industry has not been able to fully utilize …
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Effects of elevated testicular temperature on viability of cryopreserved semen and morphological characteristics of ejaculated spermatozoa
… 2 , days 12, 15...39 = Period 3. Semen was cryopreserved on each day of collection until morphological abnormalities of sperm increased to >50%. Semen viability before and after freezing was lower in Period 3 than in Period 1 (P≤.01). These differences coincided with increased abnormal …
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Effect of bioxcell and triladyl extenders and removal of seminal plasma of equilibrated and cryopreserved goat semen
The objectives of the study were to evaluate the effect of two extenders (Triladyl® and Bioxcell®) and the removal of seminal plasma on goat buck semen. Six ejaculates were collected from six indigenous bucks by means of electro-ejaculator method, and semen was pooled, and replicated 10 times. Raw …
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Effect of different disaccharides as energy supplements in tris-egg yolk semen extender on the quality of cryopreserved boer goat spermatozoa
The quality of cryopreserved Boer goat semen may be influenced by the source and concentration of energy supplements in the extender. The aim of the study was to improve the protocols for cryopreservation of Boer goat spermatozoa using different disaccharides concentrations as supplements in tris- …
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Determination of the effects of caffeine C1, 3. 7- trimethylxanthine in Tris-based egg yolk extender and different incubation intervals on the quality of cryopreserved bull semen
… and their interaction on quality parameters of cryopreserved bull semen. Semen samples were assessed for total motility, progressive motility, non-progressive motility, non-motile sperm cells, viability, acrosome integrity, and sperm cell morphological abnormalities using standard microscopic …
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High-throughput sperm cryopreservation of aquatic species
… processing systems. Large quantities of cryopreserved sperm produced from this protocol were evaluated in commercial-scale hatcheries in cooperation with aquaculture producers. To develop production for commercial requirements, quality characteristics of materials and products were …
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Cryopreservation of eukaryote algae
… algal strains tested. Of the 283 successfully cryopreserved strains comprising 240 species (68 genera), >220 species (34 genera) were cryopreserved for the first time. In addition, preliminary data suggest that some relationships exist among proposed systematic evolutionary lines, environmental …
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Development of Optimal Electromagnetic Resonance Rewarming Technology for Cryopreservation
… indispensable to maintain the integrity of the cryopreserved biological matrix. The major challenges for traditional convective rewarming method, i.e., water bath, lie in that the high specific heat of cryopreserved materials likely result in a slow warming by the unsatisfactory heat source; and …
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Effect of seminal plasma on cryopreservation and function of bovine spermatozoa
… VCL; and straight line velocity, VSL) of cryopreserved ejaculated spermatozoa (Experiment 1) and cauda epididymal spermatozoa, both fresh and frozen (Experiment 2).2) determine the effect of additional SP on sperm transport in the female using cryopreserved ejaculated sperm (Experiment 3) …
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Kryokonservierung von hepatischen Sternzellen der Ratte
… showed no significant differences between the cryopreserved and untouched cells. Neither the cell morphology nor the biochemical and cellular qualities are impaired by the cryopreservation process. The storage of cryopreserved HSC showed that a longtime storage of HSC between one till six …
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The influence of fixation and cryopreservation of cerebrospinal fluid on antigen expression and cell percentages by flow cytometric analysis
… leukocytes and brain derived cells in 1) cryopreserved CSF in comparison to freshly processed CSF, and 2) Transfix-treated CSF in comparison to freshly processed CSF. Method: CSF samples were prospectively collected and processed as follows 1) Freshwithin 24 hours (the current gold …
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Strategies to improve artificial insemination by donor
… infertility. The introduction of quarantined, cryopreserved semen and the associated reduction in cycle fecundity when compared to fresh semen necessitated the development of strategies to improve the performance of frozen sperm. A prospective randomised clinical trail was undertaken in the …
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Ex Vivo Expanded Cord Blood Natural Killer Cells As A Novel Therapeutic For Multiple Myeloma
… vivo</em> expansion of NK cells from fresh and cryopreserved CB. Using artificial antigen presenting cells (aAPCs), interleukin-2 (IL-2) and a gas permeable culture system we were able to expand CB-NK cells 1848-fold (fresh CB) and 2389-fold (cryopreserved CB). The resultant cells were >95% pure …
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Isolation, Culture and Cryopreservaion of Neural Stem Cell
… study, we investigated whether the NSCs from cryopreserved fetal brain tissue or cultured neurospheres maintain the differentiation potential compared with fresh(not frozen) tissue derived NSCs. Neural stem cell was prepared from gestation 10 to 15 weeks human fetal brain. Cultured neural stem …
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Comparison of epididymal and ejaculated sperm collected from the same Holstein bulls
… mature, fertile, Holstein bulls were collected, cryopreserved, cultured and used for in vitro fertilization. In Experiment I, epididymal sperm was found to have higher post-thaw motility compared to ejaculated sperm. During cryopreservation, the membrane permeability of ejaculated and epididymal …
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HUMAN WHARTON'S JELLY MESENCHYMAL STEM CELLS (WJ-MSCs): CHARACTERIZATION AND PROPERTIES
… sources and they can be easily cultured or cryopreserved. Many other properties make UC cells a comparatively more desirable therapeutic agent. However, more research is needed in order to elucidate the biological features and specific markers expressed by specific UC MSCs named Wharton …
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