Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"cryogenic electron microscopy"”.
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Riemannian geometry for inverse problems in cryogenic electron microscopy
… geometric approach to inverse problems in cryogenic electron microscopy (Cryo-EM). It is divided into two parts, motivated by the sub problem of orientation estimation and that of modelling of protein dynamics. The thesis is concluded with a discussion on how to bring these pieces together …
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Synthesis and performance of novel supramolecular tools for single-particle cryogenic electron microscopy and drug and gene delivery
<p>High-resolution biomacromolecular structure elucidation is fundamentally important to structure-based drug design and basic research into complex biochemical processes. Cryo-EM is an emerging alternative to XRD and NMR that is complementary in many ways relative to XRD and NMR. Materials …
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Structural insights into microbial one-carbon metabolic enzymes: Ni–Fe–S-dependent carbon monoxide dehydrogenases and acetyl-CoA synthases
… binds CODH in acetogens. In this work, we use cryogenic electron microscopy to capture the first-ever snapshot of an archaeal CODH/ACS complex. We observe a hydrophobic cavity between the CODH and ACS active sites that is rerouted relative to bacterial CODH/ACSs but conserved with a channel …
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Native chromatin extraction and its structure analysis
… in less than 2h. Finally, we further applied cryogenic electron microscopy to analyze higher order chromatin structure in near-native state. We found that the chromatin fibers hold a branching structure and this structure generally exist in eukaryotic cells.
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Use solid-state NMR to study the molecular structures of disease-associated peptide aggregates
… the oligomer was proposed based on NMR data and cryogenic electron microscopy (cryo-EM) 2D classification. The novel structural model can explain several critical phenomena about the cytotoxicity of the oligomers. Last, with the help from Dr. Lieberman’s lab, we explored the atomic structure of …
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Elucidating the biology of G-protein coupled receptor 35
… in an effort to elucidate its structure using cryogenic electron microscopy.
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Towards Establishing Methods of Synthesis, Purification, and Structural Determination for Human Fibrinogen
… a variety of complex media. Chapter 3 describes cryogenic electron microscopy and our process of obtaining a high-resolution, in-solution structure of human fibrinogen's D region. This structure is currently the highest resolution structure of any fibrinogen domain obtained in the native, …
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Deep learning methods to study structurally heterogeneous macromolecules in vitro and in situ
… average structure. However, recent advances in cryogenic electron microscopy (cryo-EM) provide avenues to determine sets of heterogeneous structures from a single dataset, and simultaneous advances in cryogenic electron tomography (cryo-ET) enable the resolution of macromolecules in their native …
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Structural and Dynamic Proximal Proteomic Analysis of TRPV2 Ion Channel Activation
… functional mechanism for TRPV2 channel opening, cryogenic-electron microscopy (cryoEM) was used to determine the structures of TRPV2 upon pharmacological activation. Using this cutting-edge structural technique, we were able to elucidate high resolution structures of TRPV2 bound to the …
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INVESTIGATION OF CRYOGENIC SURFACE CHARGING DURING OFF-AXIS ELECTRON HOLOGRAPHY
Cryogenic electron microscopy (cryo-EM) is an increasingly popular technique for determining the structure of solutions, especially of those with protein solutes. The ability to immobilize a fluid and attain atomic resolution of solutes within has propelled the technique to the forefront of …
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Deep learning for image processing in optical super-resolution microscopy
Optical microscopy is fundamentally governed by a trade-off between image quality, imaging speed and duration. The quality can be considered a function of the signal-to-noise ratio, contrast and image resolution, which are all limited by the amount of light that can be acquired within a set …
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Structural Characterization of Plaque Seeded Amyloid-β Fibrils by Magic Angle Spinning NMR
… the region from D23-A42. We have also collected cryogenic electron microscopy (cryo-EM) images of the seeded fibrils. Even though the NMR spectra show a single set of peaks, we have been unable to reconstruct high resolution electron density maps due to heterogeneity in the width and twist of the …
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CRYOGENIC MICROSCOPIES OF ENERGY STORAGE MATERIALS: INSIGHTS INTO METAL ANODES, SOLID-ELECTROLYTE INTERFACES, AND MXENES
… interfaces (SEI), and MXenes through developed cryogenic characterization techniques in batteries.Our research addresses a critical characterization gap by developing and adapting multiple cryogenic techniques specifically for energy materials research applications. By integrating cryogenic …
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Structure and regulatory mechanism of human uncoupling protein 1
… crystals for X-ray crystallography, but later cryogenic electron microscopy (cryo-EM) and single particle analysis were used to determine the structure. Human UCP1 was purified from yeast mitochondria and modified with nanobody fiducials to develop a sample suitable for cryo-EM analysis. The …
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Cryo-EM studies of class A GPCRs: the thyroid-stimulating hormone receptor and the β1-adrenoceptor
… While methodological advances, particularly in cryogenic electron microscopy (cryo-EM), have made more GPCRs amenable to structural studies, they remain challenging targets. During this project, the thyroid-stimulating hormone receptor (TSHR) and the β1-adrenoceptor (β1AR) were studied. The TSHR …
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Investigating the function of the multidrug transporter ABCG2
… Recent structural information about ABCG2 from cryogenic electron microscopy has revealed conformational changes associated with transport. However, there has been little investigation of the driving forces behind the process. ATP hydrolysis alone is traditionally thought to drive ABC …
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Cryo-EM reveals how adeno-associated virus engages its receptor AAVR and an investigation of the GPR108 ectodomain
… many AAV serotypes, including AAV2. Utilizing cryogenic electron microscopy (cryo-EM) we explore the AAV5-AAVR interactions, using an AAV5-AAVR complex, and visualize how the previously unseen PKD1 domain engages with some AAV serotypes. We then observe how a never-before visualized AAV …
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Excitons in Self-Assembled Hybrid Systems of Molecular Aggregate Light-Harvesting Nanotubes: Charge-Transfer Substrates and Solution-Stabilized Nanocomposites
… design principles for applications in optoelectronic devices have been the supramolecular structures fragility, and the Frenkel excitons delicate nature. The structural instability, both dis-assembly and spontaneous re-assembly of aggregates. Analysis of the absorption spectrum of a model …
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Structural investigations of adenosylcobalamin-dependent enzyme maturation
… for cofactor loading and unloading. Using cryogenic electron microscopy, we obtain structural data on IcmF that show that the monomeric G-protein domains of IcmF dimerize to resemble the active conformation of dimeric MeaB, and that this “active” conformation of the G-protein domains …
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Structural and functional role of the extracellular loops and C-terminal domain of the voltage-gated sodium ion channel
… In the Nav1.5 structure determined by cryogenic electron microscopy (cryoEM), the wild-type residues that correspond to some of these mutants form a complex salt bridge at the interface between the DII and DIII turret loops. Furthermore, adjacent aromatic residues could potentially form …
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