Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"cryo-microscopy"”.
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A Geometric Approach for Deciphering Protein Structure from Cryo-EM Volumes
Electron Cryo-Microscopy or cryo-EM is an area that has received much attention in the recent past. Compared to the traditional methods of X-Ray Crystallography and NMR Spectroscopy, cryo-EM can be used to image much larger complexes, in many different conformations, and under a wide range of …
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Methods for time-resolved cryo-EM using pulsed infrared radiation and vapour phase deposition
Electron cryo-microscopy (cryo-EM) is an imaging technique for determining high spatial resolution structures of proteins. Technological advances have made it possible to attain atomic resolution and attention may now be directed towards the challenge of extending the time resolution of cryo-EM …
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Machine learning for cryo-EM structure determination
Electron cryo-microscopy (cryo-EM) has revolutionised structural biology thanks to its relaxed sample requirements. Recent advances in cryo-EM image processing have enabled the structural determination of many macromolecules that were previously intractable by other methods. At the same time, …
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A Structural and Computational Study of GABAA Receptor Diversity
… remain elusive. In this thesis, electron cryo-microscopy (cryo-EM) was used to structurally characterize synaptic and extrasynaptic receptors isolated from cells expressing various combinations of $\alpha$1, $\alpha$4, $\beta$3, $\gamma$2 and $\delta$ subunits. I discovered that, from a …
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Keeping It Together and Taking It Apart: Structural Investigations of Replisome Complexes Involved in DNA Replication Fork Protection and Termination
… facilitated by the development of electron cryo-microscopy (cryo-EM), our understanding of replisome structure remains in its infancy. Here I present high-resolution cryo-EM structures of the most complete replisome complexes to date, which are involved in multiple aspects of DNA replication …
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Studies on assembly and genetic variation in mitochondrial respiratory complex I
… mortality rates. Recent high-resolution electron cryo-microscopy structures of mammalian complex I provide a foundation for both interpreting biochemical and biomedical data and understanding the catalytic mechanism. First, this thesis explores how the flavin cofactor is inserted into the …
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Development of in vitro iCLIP techniques to study spliceosome remodelling by RNA helicases
… of a larger spliceosomal complex by electron cryo-microscopy by competitors. Regardless, the role of Snu114 in spliceosome activation remains elusive. In a short section of this thesis, genetic and biochemical analysis suggest Snu114 to be a pseudo-GTPase, precluding a role for …
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Mechanisms of human RAD51 regulation by RAD52 and BRCA2
… collaborative experiments deploying electron cryo-microscopy to determine structural mechanisms underlying the regulation of RAD51 filament assembly by BRCA2. A high-resolution structure from a complex of RAD51, ssDNA and the BRC repeats of BRCA2 suggests that BRCA2 BRC repeats may promote …
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Enhanced Portability and Anti-Frosting Functionality of Cryostats for Synchrotron-Based X-ray Imaging
… state during X-ray imaging is to keep them cryogenically frozen. We have developed affordable cryostats and novel protocol to not only improve cryo-imaging at current third-generation synchrotrons, but also enable cryo-imaging at existing synchrotrons that have limited accessibility. This …
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Computational Development for Secondary Structure Detection From Three-Dimensional Images of Cryo-Electron Microscopy
<p>Electron cryo-microscopy (cryo-EM) as a cutting edge technology has carved a niche for itself in the study of large-scale protein complex. Although the protein backbone of complexes cannot be derived directly from the medium resolution (5-10 Å) of amino acids from three-dimensional (3D) density …
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Activation of lipid kinase VPS34 by Rab GTPases
… II bound to Rab5 on membranes, using electron cryo-tomography and subtomogram averaging. Complex II is dynamic on membranes with only the UVRAG/Beclin 1 “adaptor arm” permanently contacting the outer leaflet of the lipid bilayer. In contrast, the VPS15/VPS34 “catalytic arm” hovers over the …
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Investigating tau propagation in situ
… To achieve this, I employed electron cryo-tomography (cryo-ET), a powerful technique that allows the visualization of biological samples at molecular resolution in near-native conditions. I wanted to study tau propagation in a disease relevant context. I first focused on human tissue …
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Architecture and assembly of maize streak virus: insights from 3D electron microscopy
… genus Begomovirus) have been studied by electron cryo- microscopy (cryo-EM) previously. While these investigations revealed some details about the geminate architecture, the interactions of capsid components have not yet been adequately modelled. The two incomplete icosahedral "heads" of the …