Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"cryo ET"”.
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ADVANCING IN SITU CRYO-ET TO THE NEXT DIMENSION: TOMOGRAPHY ON 3D ORGANOIDS
Interpreting how molecular organization supports cell and tissue function remains a central challenge in biology. In situ cryo-electron tomography (cryo-ET) can help to address this challenge by providing high-resolution, three-dimensional visualization of macromolecules in near-native, minimally …
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Linking Architecture and Function of Endoplasmic Reticulum - Plasma Membrane Contact Sites
… of eukaryotic cells form an intricate network of membrane contact sites (MCS) and thereby promote cellular functions such as lipid metabolism, signalling and organelle biogenesis. The formation of cortical endoplasmic reticulum (cER) - plasma membrane (PM) contacts in budding yeast cells …
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A CORRELATIVE CRYO-ELECTRON TOMOGRAPHY APPROACH TO STUDY BRD4-NUT CHROMATIN CONDENSATES ACROSS SCALES
… their compatibility with active transcription. Cryo-electron tomography (cryo-ET) is suitable for conducting such analyses, as it allows molecular-resolution imaging of cellular structures within their native context. However, methodological challenges limit the straightforward application of …
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Investigating the cytoskeleton and microtubule-based transport with in situ cryo-electron tomography
The cytoskeleton is composed of microtubules, actin, and intermediate filaments, which function both as a cellular scaffold and as tracks for motor proteins that drive intracellular transport. Cytoplasmic dynein, in combination with dynactin and activating adaptors, is a microtubule-based motor …
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Computational Modeling of Claudin-15 Paracellular Channels
… tissues through claudin protein assemblies, yet the molecular basis of strand flexibility, pore architecture, and ion selectivity for cation-selective claudin-15 (Cldn-15) remains incompletely defined at atomic resolution. This dissertation employs molecular dynamics (MD) simulations, reduced …
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Investigating tau propagation in situ
… To achieve this, I employed electron cryo-tomography (cryo-ET), a powerful technique that allows the visualization of biological samples at molecular resolution in near-native conditions. I wanted to study tau propagation in a disease relevant context. I first focused on human tissue …
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Deep learning methods to study structurally heterogeneous macromolecules in vitro and in situ
… macromolecular complexes are often structurally heterogeneous, which is key to executing or regulating their specific biological functions. To understand the molecular mechanisms underpinning these biological functions, structural biologists aim to determine the 3-D structure of the relevant …
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Structural and biochemical characterization of nuclear pore complex structural scaffold sub-complexes
… and human diseases associated with deletions or mutations of constituents. To obtain detailed structural information of the NPC, techniques that span several resolution ranges are necessary due to its large size and complexity. For instance, recent progress in the structural …
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Molecular Architecture of the ER-Mitochondria Encounter Structure
Membrane contact sites (MCS) form between all organelles of eukaryotic cells and facilitate interdependent processes between unique subcellular compartments. In budding yeast, MCS between the endoplasmic reticulum (ER) and mitochondria are mediated by a protein complex known as the ER-mitochondria …
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Cryo-EM reveals how adeno-associated virus engages its receptor AAVR and an investigation of the GPR108 ectodomain
… some aspects of its basic biology are still not yet fully understood. Only recently in 2017 was the primary receptor for most AAV serotypes discovered and there are still unknown factors in AAV cell entry, uncoating, and host protein interactions. Furthermore, its use in gene therapy can be …
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Towards structural characterisation of ciliary rootlets, ciliary caps and intraflagellar transport
… of building blocks and signalling molecules between the ciliary base and tip through a process called intraflagellar transport (IFT). IFT is driven by multiple copies of the ciliary motor proteins kinesin-2 and dynein-2 attached to train-like assemblies of IFT-A and IFT-B protein subcomplexes. …
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Visualisation of innate immune signalling complexes in cells by cryo-electron tomography
… functional and fluorescently labelled MAVS yet, it provides solid foundation for future labelling experiments of MAVS. I optimised a cryo-correlative light electron microscopy (cryo-CLEM) workflow to attain high-resolution structural insights into the ASC signalling complexes in cells. My …
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The encapsidation protein from Lactococcus lactis asccφ28 as a novel system for the study of molecular motors
… x-ray studies reveal a decameric stoichiometry. Surprisingly, preliminary crystallographic, as well as cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM) studies, suggest the decameric assembly is in fact composed of a dimer of pentamers, akin to the widely …
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Molecular recognition, in- and out- of equilibrium, in the actions of bacterial multidrug efflux nanomachines
… compounds are recognised, while critical metabolites and other molecules needed by the cells are retained, is one intriguing puzzle of this system. Structural information and dynamics are crucial for elucidating the functionality of this molecular machine; however, its allosteric nature and …
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Molecular modeling and simulation of bacterial chemosensory arrays
… of proteins into a highly cooperative signaling network. The present dissertation will present my work using techniques in computational modeling and simulation to investigate the molecular structure and function of the bacterial chemosensory array. A brief overview of each chapter follows. …
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Molecular mechanisms of Sorting nexin-9 in membrane triggered actin polymerisation
… correlate with several stages of breast cancer metastasis, and poor patient survival in colorectal and prostate cancer. In addition to this, SNX9 is also required for Chlamydia trachomatis entry into cells and contributes to aberrant actin assemblies in a rare disease of phospho- inositide lipid …
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Structure & Function of Bacterial Transport Machines in their Cellular Context
… antibiotic efflux pump and the Type I Secretion System (T1SS), responsible respectively for antibiotic resistance and export of protein virulence factors. To pass the Gram-negative envelope in a one-step translocation process, both machines use a tripartite system, consisting of outer …
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Cryo-electron Microscopy of Reconstituted Membrane Fusion Systems
Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins are responsible for driving the fusion of biological membranes and are present across many different cell trafficiking systems. Synaptic SNARE proteins allow the release of neurotransmitters across synaptic …
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Fantastic ribonucleoprotein complexes and how to study them. Architectural principles of regulatory assemblies in bacteria.
… (RBPs) and small regulatory RNAs (sRNAs). Together these form an information-rich regulatory network that exercises post-transcriptional control of gene expression. A key RBP in bacteria is Hfq, which is best studied as a facilitator for sRNA-mRNA pairing. In Pseudomonas aeruginosa, however, …