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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 393 for “"cryo"”.
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Cryo-EM Studies of Bacterial RNA Polymerase
… complexes to be determined by single particle cryo-electron microscopy (cryo-EM). Unlike X-ray crystallography, cryo-EM samples can be directly visualized without crystallization. In this thesis, I optimized and utilized cryo-EM methodologies to structurally characterize several bacterial RNAP …
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Machine learning for cryo-EM structure determination
Electron cryo-microscopy (cryo-EM) has revolutionised structural biology thanks to its relaxed sample requirements. Recent advances in cryo-EM image processing have enabled the structural determination of many macromolecules that were previously intractable by other methods. At the same time, …
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THz Cryo-CMOS Link for Quantum Computing
In this thesis, a terahertz (THz) cryo-CMOS link for quantum computing and other cryogenic applications is designed. This THz wireless link can efficiently deliver the control signals and data between room temperature and cryogenic environment. Its operation allows for a small antenna aperture …
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Affinity cryo-electron microscopy: Methods development and applications
<p>Single particle cryo-electron microscopy (cryo-EM) is an emerging powerful tool for structural studies of macromolecular assemblies. Although less concentrated and smaller amounts of samples are required for single particle cryo-EM compared to X-ray crystallography, it remains challenging to …
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Cryo-electron microscopy studies of pluripotency factor Nanog
… oligomerisation of Nanog drives self-renewal, cryo-electron microscopy and single particle analysis were utilised to determine a 3D structure of Nanog. By performing size exclusion chromatography and analytical ultracentrifugation, it was discovered that Nanog forms large oligomers of 860 kDa …
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Cryo-electron Microscopy of Reconstituted Membrane Fusion Systems
… of a SNARE reconstituted in-vitro system, I use cryo-electron tomography to provide insight into how SNARE proteins organise at fusion sites showing that such organisation occurs in a vesicle-plasma membrane distance-dependent manner. In order to improve the quality of structural data acquired at …
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A cylindrical specimen holder for electron cryo-tomography
… a way to make cylindrical specimens for electron cryo-tomography, using thin-walled carbon tubes as specimen holders. The tubes are made in a multi-step process, involving carbon deposition on glass micropipette templates and subsequent removal of the glass. Tube diameters are typically a few …
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Investigating Microtubules Inside Cells with Cryo-Electron Tomography
… of neuronal microtubules remain unclear. Cryogenic electron tomography allows for visualization and structure determination of purified or cellular specimen in their native-like, vitrified state. I used this method in combination with subtomogram averaging to study intracellular …
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A cylindrical specimen holder for electron cryo-tomography
… a way to make cylindrical specimens for electron cryo-tomography, using thin-walled carbon tubes as specimen holders. The tubes are made in a multi-step process, involving carbon deposition on glass micropipette templates and subsequent removal of the glass. Tube diameters are typically a few …
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Deep Learning for Segmentation Of 3D Cryo-EM Images
<p>Cryo-electron microscopy (cryo-EM) is an emerging biophysical technique for structural determination of protein complexes. However, accurate detection of secondary structures is still challenging when cryo-EM density maps are at medium resolutions (5-10 Å). Most existing methods are image …
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Cryo-electron microscopy studies on ovine mitochondrial complex I
… I was developed and suitable conditions for cryo-EM imaging established, after extensive screening of detergents and additives. Cryo-EM images were acquired with the recently developed direct electron detector and processed using the latest software. This allowed the solution of the nearly …
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2D image classification and alignment in single-particle Cryo-EM
Background and Objective: Single-particle Cryo-EM is a powerful tool for structural biologists to determine structures of macro-molecules at atomic resolution. In single-particle Cryo-EM, many individual biological molecular particles with identical structures are rapidly frozen at unknown, random …
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A Geometric Approach for Deciphering Protein Structure from Cryo-EM Volumes
Electron Cryo-Microscopy or cryo-EM is an area that has received much attention in the recent past. Compared to the traditional methods of X-Ray Crystallography and NMR Spectroscopy, cryo-EM can be used to image much larger complexes, in many different conformations, and under a wide range of …
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NUMERICAL AND EXPERIMENTAL INVESTGIATION OF CRYO-FREEZING WITH LARGE BLOOD VESSELS
Cryosurgery is accepted as a favorable treatment option for eradicating undesirable cancerous tissue due to its minimally invasive nature. A finite difference study of a biological liver tissue undergoing cryo-freezing using the immersed boundary method (IBM) is presented in this study. The liver …
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Cryo-electron microscopy image analysis using multi-frequency vector diffusion maps
Cryo-electron microscopy (EM) single particle reconstruction is an entirely general technique for 3D structure determination of macromolecular complexes. However, because the images are taken at low electron dose, it is extremely hard to visualize the individual particle with low contrast and high …
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Machine Learning for Reconstructing Dynamic Protein Structures from Cryo-EM Images
… of dynamic biomolecular complexes via cryo-electron microscopy (cryo-EM). In single particle cryo-EM, the central problem is to reconstruct the 3D structure of a target biomolecular complex from a set of noisy and randomly oriented 2D projection images, a challenging inverse problem …
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A Graph-Based Algorithm to Determine Protein Structure from Cryo-EM Data
Cryo-electron microscopy: cryo-EM) provides 3D density maps of proteins, but these maps do not have sufficiently high resolution to directly yield atomic-scale models. Previous work has shown that features known as secondary structures can be located in these density maps. A second source of …
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Understanding the Structure and Function of GABAA Receptors Using Cryo-Electron Microscopy
The general metadata -- e.g., title, author, abstract, subject headings, etc. -- is publicly available, but access to the submitted files is restricted to UT Southwestern campus access and/or authorized UT Southwestern users.
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Visualisation of innate immune signalling complexes in cells by cryo-electron tomography
… labelling experiments of MAVS. I optimised a cryo-correlative light electron microscopy (cryo-CLEM) workflow to attain high-resolution structural insights into the ASC signalling complexes in cells. My cryo-ET reconstructions, obtained in unstained and fully hydrated conditions, show that the …
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ADVANCING IN SITU CRYO-ET TO THE NEXT DIMENSION: TOMOGRAPHY ON 3D ORGANOIDS
… remains a central challenge in biology. In situ cryo-electron tomography (cryo-ET) can help to address this challenge by providing high-resolution, three-dimensional visualization of macromolecules in near-native, minimally disturbed conditions. With its broad resolution, cryo-ET enables us to …
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