Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"cre/lox"”.
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Cre-lox-basierte Inaktivierung des Pbx3-Gens
… Mäusen untersucht. <br>Zur Herstellung eines Cre-lox-basierten „Targeting“-Vektors wurde ein Teil der Genregion des Pbx3-Genes subkloniert und das Exon 3 als Zielsequenz sowie syngene DNA zur homologen Rekombination in den pFlox-Vektor eingefügt. Das linearisierte Rekombinationskonstrukt …
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Role of retinoblastoma protein in the development of liver cancer
… was determined using an in vitro system, with Cre-lox technology. The Cre-lox technology enables the lethality of Rb loss in the embryo to be overcome. The regulation of p53, extent of DNA damage and cell cycle control were investigated in Rb-/- hepatocytes. The tumor suppressor p19ᴬᴿᶠ, …
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Evaluation of the Recombination Efficiencies of FLP Proteins
… recombination systems in plants are the Cre/lox system from the bacteriophage P1 and the FLP/FRT system from the yeast Saccharomyces cerevisiae. The Cre/lox system is well-characterized and is the first choice in application of site-specific recombination system. However, some …
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Identification of novel gastric stem cell markers and investigation of Intestinal Stem Cell Cre-mediated Recombination
… Identifying new markers is important for increasing our basic understanding of gastric tissues and studying mechanisms of cancer development. Gastric and intestinal tissues share a common developmental program. Thus, intestinal stem cell genetic drivers were investigated for putative …
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The role of Wt1 in Müllerian duct development
… novel conditional null reporter allele, <em>Wt1 flox-RFP</em>. This allele utilizes the Cre-lox system to delete exons 8 and 9 of <em>Wt1</em>, disrupting the DNA binding capability of the protein. Simultaneously, a nuclear red fluorescent protein is activated in cells expressing the recombined …
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Verifying the Deletion of Growth Hormone Receptor Using a Quantitative Polymerase Chain Reaction at the mRNA Level in Tissue-Specific GHR-/- Mice
… three tissue-specific GHR-/- mice were created using a Cre-Lox system. This thesis represents the efforts to verify the deletion of GHR at the mRNA level only in the specific tissues of these tissue-specific gene-disrupted mouse lines. Collectively, these data show the GHR mRNA levels …
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The analysis of a mouse model of Lysosomal Storage Disorder uncovers a role for astrocyte dysfunction in neurodegeneration
… in neurodegenerative processes is becoming increasingly appreciated. In my PhD project, I investigated the contribution of astrocytes to neurodegeneration in Multiple Sulfatase Deficiency (MS D), a severe Lysosomal Storage Disorder (LSD) caused by mutations in the Sulfatase Modifying Factor 1 …
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THE CHARACTERIZATION OF BEHAVIORAL ABNORMALITIES IN BDNF LOXP TRANSGENIC MICE
<p>BDNF homozygous floxed mice (BDNF<sup> lox+/+</sup>) are a transgenic mouse strain used to study the neurotrophin brain-derived neurotrophic factor (BDNF) through Cre-Lox recombination when crossed with the appropriate Cre-expressing strain. BDNF<sup> lox+/+</sup> mice contain two artificially …
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THE ROLE OF SKELETAL MUSCLE-SYNTHESIZED BRAIN-DERIVED NEUROTROPHIC FACTOR IN RETROGRADE TRANSPORT ALONG MOTORNEURON AXONS
… and reduced BDNF is implicated in NMDs. Using Cre-Lox gene recombination technology our lab generated transgenic mice in which the BDNF gene was knocked out of skeletal muscle. We hypothesized that skeletal muscle-BDNF is required for normal retrograde transport of proteins and mitochondria …
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THE ROLE OF SKELETAL MUSCLE SYNTHESIZED BRAIN-DERIVED NEUROTROPHIC FACTOR (BDNF) ON THE HEALTH AND MAINTENANCE OF THE MURINE MOTOR UNIT
… To investigate this, we used a mouse model with Cre/Lox targeted gene deletion driven by the human alpha skeletal actin promoter, resulting in experimental animals with reduced or absent muscle-derived BDNF (MuscleBDNF<sup>+/-</sup>, MuscleBDNF<sup>-/-</sup>). We examined gastrocnemius and soleus …
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Role of entF Gene in Iron Acquisition by Brucella abortus 2308
… (BAN1) of B. abortus 2308, generated using cre-lox methodology was found to be growth inhibited in iron minimal media compared to wild type strain. Growth inhibition was further enhanced with the addition of an iron chelator or 0.1% erythritol. Compared to wild type strain, no growth …
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Development and applications of programmable DNA-guided Argonaute-based artificial restriction enzymes
… as AREs. Through these studies, I was able to create an engineered version of PfAgo enzyme which demonstrates lower non-guided nuclease activity as well as higher specificity for cleavage of high GC-content DNA sequences compared to the wild-type PfAgo enzyme. To demonstrate some of the …
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Kasvipatogeenin Pseudomonas syringae pv. tomato DC3000 -bakteerin tuottamien Hop-efektoriproteiinien merkitys taudin syntyyn
… aloitettu antibioottimarkkerin poisto Cre-lox-mekanismilla.
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Elucidating the role of SRSF1, a prototypical splicing factor, in liver physiology
… has the remarkable capability to circumvent the Cre-lox based knock-out of SRSF1. However, the chronic cycle of death and regeneration leads to the development of NASH-like pathology. To understand primary changes occurring in the model, an acute SRSF1 knock-out model was developed. Insight into …
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Stress and inflammation regulate the kynurenine pathway dioxygenases in a glia‐ and tissue‐specific manner
… between symptoms of depression and increased Kynurenine Pathway activity, the cellular specificity underpinning DO regulation by stress and inflammation remain poorly defined. Thus, (Chapter 1) after a review of the relevant literature, this thesis probes the regulation of DO …
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The effect of brain-specific VEGF-C deletion on vascular morphology in the brain and skull
… role of VEGF-C in the brain, we generated Nestin-Cre; Vegfcfl/fl mice, in which we confirmed deletion of Vegfc. Nestin-Cre is expressed in neuronal and glial cell precursors, in which it mediates Cre-lox recombination of the floxed Vegfc allele. The objective of my study was to see if …
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Yap and Taz Are Required For Neural Crest-Derived Cardiovascular Development
… cause of early infant death. Cardiac neural crest cells (NCCs) are a migratory and multipotent cell population known to aid in the development of the cardiac outflow tract (OFT), valves, and interventricular septum, during embryogenesis. <em>Yap </em>and <em>Taz </em>are downstream effectors …
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Understanding the pathogenesis of myotonic dystrophy type 1
… pathology. To do this, we attempted to use the Cre-lox system for the conditional expression of an EGFP reporter-linked expanded CUG repeat RNA in the mouse. Expression of the transgene was controlled by Cre excision of a transcriptional stop, placed upstream of the EGFP-expanded repeat open …
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Abscisic acid ameliorates glucose tolerance and obesity-induced inflammation
… a tissue-specific knockout generated through Cre-lox recombination, we were able to determine that this effect was dependent on PPARgamma in immune cells. To further characterize the differences between the ATM subsets that were affected by ABA, we performed a multi-organ assessment (i.e., …
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Variation in chromosomal aberration induction in differing ATM and ASPM genotypic backgrounds detected via a modified PNA FISH technique
… and exposed to 0.2 μM tamoxifen for inducible Cre-lox excision of floxed Aspm alleles. Upon analysis of chromosome-type aberrations, there were statistically significant elevations in acentric fragment and/or dicentric average frequencies seen across all strain backgrounds of the Atm-/- …
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