Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"cpn60"”.
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Analysis of the multiple chaperonins of Mycobacterium smegmatis
… gene for the essential oligomeric chaperonin Cpn60, many contain two or more cpn60 genes. The non-pathogenic Mycobacterium smegmatis has three cpn60 homologues, while the pathogenic Mycobacterium tuberculosis has two. This study is a functional characterisation of the chaperonins of M. …
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Microbial profiling using metagenomic assembly
… was undertaken to formally evaluate 16S rRNA and cpn60 as DNA barcodes for Bacteria. Both 16S rRNA and cpn60 were found to meet the criteria for DNA barcodes, with cpn60 a preferred barcode based on its superior resolution of closely related taxa. The high resolution of cpn60 enabled a method of …
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Phenotypic and genetic diversity of pseudomonads associated with the roots of field-grown canola
… were analyzed according to their 16S rRNA and cpn60 gene sequences and selected phenotypic properties (fatty acid methyl ester (FAME) profiles, antibiotic resistance, extracellular enzyme production and carbon substrate utilization). On the basis of 16S rRNA and cpn60 gene sequences, two major …
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SEX-DEPENDENT GUT MICROBIOME DIFFERENCES IN A TRANSGENIC MOUSE MODEL OF AUTISM
… and gut microbiome composition, we performed cpn60 amplicon sequencing on fecal samples taken from transgenic mice expressing human sAPPα(NtgsAPPα = 10; Nwt = 18). We found no evidence of a strong effect on alpha or beta diversity, but did find significant reductions in the proportional …
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Characterization of the vaginal microbiome in pregnancy
… in pregnancy has been established based on cpn60 gene amplicon sequencing. The vaginal microbiota of pregnant women relative to non-pregnant women had lower richness and diversity, higher Lactobacillus abundance and lower Mollicutes/Ureaplasma prevalence. This gives a better understanding of …
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Characterization of putative sialidase genes in Gardnerella vaginalis
… been divided into four subgroups (A-D) based on cpn60 universal target sequencing. Recent research has demonstrated that a previously identified sialidase gene (Gene 1) does not correlate with sialidase activity. Analysis of 39 available G. vaginalis genome sequences identified a second sialidase …
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Characterizing the genotypic and phenotypic diversity of Gardnerella vaginalis from vaginal clinical samples
… genotyped using their chaperonin-60 (cpn60) sequences, revealing the presence of four phylogenetic clades (subgroups A-D) made up of 13 subgroup A, 17 subgroup B, 58 subgroup C and 2 subgroup D isolates. To determine the phenotypic diversity of G. vaginalis isolates, sialidase …
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Characterisation of vaginal Prevotella strains from a cohort of South African adolescent girls and young women with and without bacterial women with and without bacterial vaginosis
… defence mechanisms. Phylogenetic analysis by cpn60 gene sequencing and core genome alignment revealed that intra-sample diversity was generally low, but at least one sample had two distinct strains at the same time point. The putative P. melaninogenica isolate was most closely related to …
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Determination of factors affecting the community structure of Gardnerella spp. in the vaginal microbiome
… roles in the pathogenesis of vaginosis. Four cpn60-defined subgroups: A, B, C, and D, corresponding to thirteen “genome species” have been identified. Multiple subgroups of Gardnerella usually colonize vagina simultaneously. Subgroups A and C are the most common and abundant, while subgroup D …
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THE MICROBIOME AND THE REPRODUCTIVE ECOLOGY OF Dermacentor variabilis AND Dermacentor andersoni IN WESTERN CANADA
The geographical ranges of the Rocky Mountain wood tick, Dermacentor andersoni, and the American dog tick, Dermacentor variabilis, are largely allopatric on the Canadian prairies. Range expansion by D. variabilis to the north and west has resulted in an area of sympatry in south-western …