Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"covalent labeling"”.
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Development and Application of Covalent-Labeling Strategies for the Large-Scale Thermodynamic Analysis of Protein Folding and Ligand Binding
<p>Thermodynamic stability measurements on proteins and protein-ligand complexes can offer insights not only into the fundamental properties of protein folding reactions and protein functions, but also into the development of protein-directed therapeutic agents to combat disease. Conventional …
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Ion Mobility and Gas-Phase Covalent Labeling Study of the Structure and Reactivity of Gaseous Ubiquitin Ions Electrosprayed from Aqueous and Denaturing Solutions
Gas-phase ion/ion covalent modification was coupled to ion mobility/mass spectrometry analysis to directly correlate the structure of gaseous ubiquitin to its solution structures with selective covalent structural probes. Collision cross-section (CCS) distributions were measured prior to ion/ion …
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Molecular actions of estrogen agonists and antagonists and their interaction with the human estrogen receptor
… followed by analysis of the hormone binding, covalent labeling and transactivating ability of the mutant receptors. Mutagenesis revealed that Cys530 is the preferential site of labeling by covalent affinity labels, however, in the absence of a cysteine residue at postion 530, Cys 381 is …
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Statistical and computational approaches for the analysis of high-throughput epigenomic data /
… is the first DNA sequencing method which uses covalent labeling of unmodified individual CG sites, following by the synthesis of DNA polymerase. This DNA sequencing method combines the enrichment of the unmodified DNA fraction, the resolution of a single base, and the strand specificity. …
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Protein Structural Studies by Oxidative Labeling and Mass Spectrometry
… subject in biological chemistry. Oxidative labeling combined with electrospray ionization (ESI) mass spectrometry (MS) offers enormous opportunities for studying protein structures at the level of individual residues. Such high selectivity combined with excellent sensitivity makes ESI-MS a …
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Applications of functional nucleic acids in imaging, sensing and drug delivery and investigations of DNAzyme-metal interactions
… Most FNA-based fluorescent sensors require covalent labeling of fluorophore/quencher to FNAs, which incurrs extra expenses and could interfere the function of FNAs. Chapter 3 describes a new sensor design avoiding the covalent labeling of fluorophore and quencher. The fluorescence of …
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Investigations of the inhibition mechanisms of human ribonucleotide reductase by gemcitabine-5'-diphosphate and saccharomyces cerevisiae ribonucleotide reductase by Sml1
… of a reductant. Inactivation is caused by covalent labeling of RNR by the sugar of F2CDP (0.5 equivalents/[alpha]) and is accompanied by the release of 0.5 equivalent cytosine/[alpha]. Studies using size exclusion chromatography reveal that in the E. coli RNR, an u212 tight complex is …
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Insights into the substrate specificities, interactions and regulatory mechanisms of bacterial glycoconjugate biosynthesis enzymes
… superfamily from a cellular proteome. Robust, covalent labeling at the active site of various representative monoPGTs from unfractionated cell membrane fractions is demonstrated using 3- phenyl-2H-azirine probes.
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Nanoscale protein analysis utilizing capillary electrophoresis and laser-induced fluorescence detection
… with valuable tools in protein analysis. Various covalent and non-covalent fluorescent derivatization approaches have been investigated, with emphasis on biochemical and/or clinical applications. The non-covalent dye, NanoOrange, is used as a clinical diagnostic tool for early disease diagnosis, …
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Synthesis and characterization of new light emitting probes for sensitive detection of bio-molecules and live cells
… and biological applications take advantage of labeling the objects of interest with fluorescent or luminescent tracers. Luminescent tracers take advantage of the unique property of some lanthanide metals to absorb and emit light. Long lifetime of lanthanide emission allows temporal gating of …
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Single Molecule Study of Polymer-Surfactant Interactions
… systems can be followed by FCS without covalent labeling with dye molecules. Moreover, FCS is only sensitive to the dye concentration; therefore these investigations can be applied over a wide range of polymer concentrations.