Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"costimulation"”.
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Understanding the function of ICOS/ICOSL costimulation in experimental autoimmune myasthenia gravis
… we have characterized the role of ICOS/ICOSL costimulation in experimental autoimmune myasthenia gravis (EAMG), a model of human MG. Following acetylcholine receptor (AChR) immunization, ICOS gene-deficient mice were resistant to the development of EAMG due to faulty germinal center formation, …
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Studies of human T cell costimulation: potential for the immunotherapy of cancer
Costimulation is required for the generation of an effective T cell based immune response. The presentation of tumour associated antigens may occur in the absence of effective costimulation, inducing tolerance. Conversely effective costimulation can overcome immunosuppressive mechanisms present …
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Investigating the role of CD28 costimulation and IL-4/IL-13 responsive myeloid and lymphoid cells during helminth infections in mice
The aim of this study was to evaluate the importance of CD28 in initiating protective Th2 immunity against both primary and secondary infections with N. brasiliensis. Our findings demonstrate that CD28 is required for initiation of protective Th2 immunity against primary infection with N. …
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Spatial Dynamics and the Mechanoresponse in CD4+ T Cell Activation
… TCR engagement and is further augmented by CD28 costimulation: ensuring T cell survival and conferring numerous functional capabilities. The work in this dissertation highlights the spatial and temporal dynamics that regulate the initial coupling of CD28 with TCR signaling and also dissects the …
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Cellular and Molecular Aspects Of Naïve T cell Priming for Adoptive Immunotherapy
… CD62L and CCR7 as well as the important costimulation protein CD28. However, low dose IL-15 during primary and secondary expansion results in improved cell yield while preserving a population of central memory phenotype. To further develop our ex vivo protocol we investigated the effect …
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The Characterization of T-Cell Manufacturing For Adoptive T-Cell Therapies
… better to T-cell manufacturing involving CD28 costimulation, while older patients appear to respond better to T-cell manufacturing lacking CD28 costimulation.</p>
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Study and Therapeutic Interference of T Cell Function in Pressure Overload-induced Heart Failure
… We inhibited T cell function in vivo via T cell costimulation blockade, through FDA-approved rheumatoid arthritis drug abatacept (CTLA-Ig). Administration of abatacept led to highly significant delay in progression and decreased severity of cardiac dysfunction in the mouse HF model. The …
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Staphylococcal Enterotoxin B Induced Clonal Anergy in αβ T Cell Receptor Transgenic Mice
… was due to T cell activation in the absence of costimulation, we examined the effect of the CD28/B7 pathway on the response of T cells to SEB and OVA. The T cell response to both OVA and SEB was enhanced by antibodies to the CD28 coreceptor suggesting that CD28/B7 mediated costimulation plays a …
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Investigating immune surveillance, tolerance, and therapy in cancer
… SIY-specific T cells required a higher level of costimulation to achieve functional activation.
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Tethered Il-15 to Augment The Therapeutic Potential of T Cells Expressing Chimeric Antigen Receptor: Maintaining Memory Potential, Persistence, and Antitumor Activity
… to the cell surface would enhance T-cell costimulation to support persistence independent of CAR activation by preserving T-cell memory potential and maintain antitumor activity in the presence of low TAA. Using clinically compliant methods, the generated mIL15-CAR T cells mimicked the …
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Overcoming T Cell Tolerance Against Endogenous Choroid Plexus Tumors Using a Combination Immunotherapy
… and tumor T cell infiltrate. The addition of costimulation through an anti-CD28/Folate bispecific conjugate, PV-1 Fab/Folate, further enhanced survival and dramatically slowed tumor growth. Furthermore, proliferating CD8 T cells were found in tumors of treated animals. However, upon treatment …
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Signalling reactions of PI3Kgamma in T cells
… upon T cell receptor (TCR) stimulation. Costimulation with specific anti-CD28 antibody or activation with Ionomycin and phorbol ester is able to rescue this proliferation, in contrast to subsequent interleukin-2 (IL-2) and interferon-gamma production. In our experiments IL-2 costimulation …
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Reciprocal Regulation of CD4+ and CD8+ T Lymphocyte Effector and Memory Fates by Interleukin 12 and Type 1 Interferon
… a novel model in which TCR activation and costimulation act to shape the ability for IL-12 and IFN-a/b to differentially program the development of distinct classes of effector and memory CD8+ T lymphocytes. These studies have direct bearing on the design and development of effective …
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The Role of Alternative Splicing and Alternative Polyadenylation in Regulating Activated T cell Biology
… events, ~10-30%, are regulated by CD28 costimulation. Through the creation of CRISPR clones and AMO transfection experiments, I excitingly found isoforms of Caspase-9, Bim, and Bax, that increase in abundance upon T cell stimulation and work in an additive manner to promote T cell …
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Expression Profiles of Molecules Involved in Antigen Presentation in Gangliosides-stimulated Primary Astrocytes from Rat brain
… Cell surface molecules of central importance to costimulation are CD40, ICAM-1, CD80 (B7-1) and CD86 (B7-2). In astrocytes, mRNA expressions of the above molecules were constitutive and that of CD86 was further induced by treatment of Gmix. Those expression patterns are the same in …
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Retroviral-mediated gene transfer of Il-1β?cDNA into human activated T cells
… (mAb) plus interleukin-2 (IL-2), or 2) cis costimulation using beads carrying co-immobilized anti-CD3 and anti-CD28 mAbs. By cross-linking the CD3 and CD28 receptors, these mAbs mimic in vivo signaling events, leading to cytokine production and proliferation. A modified human interleukin-1β …
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Studies of the Mechanisms of Reverse Signaling by CD40L
… neither mutation affected the ability of costimulation to mobilize intracellular CD40L to the cell surface. However, the deletion of the cytoplasmic domain reduced overall protein expression, indicating a potential role for the cytoplasmic domain in CD40L stability or translation …
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Functional characterization of rat CTLA-4 and CD25+CD4+ regulatory T cells
… of anti-TCR- and anti-CD28 stimulated (costimulation) T cell responses such as reduction in activation marker expression, proliferation, and cytokine IL-2 production. Although T cells stimulated with the superagonistic anti-CD28 antibody alone without TCR engagement also increased their …
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The Genetic, Molecular, And Cellular Bases Of Unidentified Primary Immunodeficiencies
… pathways and reducing the requirement for T cell costimulation. I also identified homozygous missense mutations in IFIH1, the gene encoding the cytosolic pattern recognition receptor of dsRNA MDA5, in the third family of study. The affected individual presented with recurrent severe respiratory …
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Costimulatory function of CD44
… receptor, which could be greatly enhanced by costimulation via CD44. On the level of signal transduction, coligation of CD44 with CD3 resulted in a strong and sustained increase of early tyrosine phosphorylation events and upregulated downstream signal transduction pathways, such as the …
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