Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"constitutive promoter"”.
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Functional analysis of soybean PR-10 genes
… vector, expression controlled by the CaMV35S constitutive promoter. Four PR-10 family members were transformed into Arabidopsis thaliana, and alterations in defense responses were monitored in the PR-10 transformants. Two RNAi constructs were made for future transformation into soybean, to …
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Improving barley for biofuel production – investigating the role of 4CL and CCR in the lignin biosynthesis pathway
… was used to down-regulate 4CL1 and CCR1 using a constitutive promoter via Agrobacterium-mediated transformation of barley. From an extensive screen of the primary transformants for changes in protein level and lignin content, six CCR and four 4CL lines were taken forward for detailed analysis. …
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Molecular and Functional Characterization of Terpene Chemical Defense in Arabidopsis Roots in Interaction with the Herbivore Bradysia spp. (fungus gnat)
… synthase AtTPS08, which is responsible for the constitutive formation of the novel volatile diterpene compound, rhizathalene, in Arabidopsis roots. Rhizathalene synthase is a class I diterpene synthase that has high affinity for the substrate geranylgeranyl diphosphate (GGPP) and is targeted to …
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Heterologous Expression of Arabidopsis Thaliana Purple Acid Phosphatase Gene (Atpap15) in Crops for Phytoremediation of Sites Contaminated with Excess Phosphorus
… under cauliflower mosaic virus (CaMV35S) constitutive promoter. After PCR confirmation of the gene, plants were transferred to the greenhouse and allowed to grow in pots. The pots contained Sta-Green potting mix (Lowe’s Inc., Mooresville, North Carolina,U.S.). Individual flowers were …
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Genetic analysis of RNA polymerase/transcriptional activator interactions at thepepT promoter of S. typhimurium
… In addition, we have characterized the pepT promoter region. Mutational studies and primer extension analysis demonstrate that pepT is transcribed from two promoters, an FNR-dependent, anaerobically induced promoter (P1), and a constitutive promoter (P2) conferring low level expression. A …
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Transcriptional targeting of lentiviral vectors to the erythroblastic progeny of hematopoietic stem cells
… of the LTR, immediately upstream of the HIV-l promoter. The modified LTR was used to drive the expression of a reporter gene (GFP), while a second gene (~LNGFR) was placed under the control of an internal, constitutive promoter to monitor cell transduction, or immunoselect transduced cells, …
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A Physiological, Biochemical and Structural Analysis of Inositol Polyphosphate 5-Phosphatases from Arabidopsis thaliana and Humans
… expressing At5PTase1 under the control of a constitutive promoter were characterized. This characterization revealed that plants ectopically expressing At5PTase1 had an altered response to ABA. These plants have stomata that are insensitive to ABA, and have lower basal and ABA-induced …
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Engineering Zymomonas mobilis for the production of biofuels and other value-added products
… production in Z. mobilis, we have constructed a constitutive promoter library that leads to a range of gene expression levels in the strain. Collectively, our results provide the framework for the development of an industrial production process utilizing Z. mobilis as the microbial host.
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IN VIVO IMAGING OF STEM CELL MEDIATED TREATMENT IN A MOUSE MODEL OF SPINAL CORD INJURY
… a viral vector expressing Luciferase under a PGK constitutive promoter (PLW vector). Results: the iron content/cell increased in proportion to the incubation time and to the iron concentration in the medium and in relation to different carriers (PLL, PB and PS) in labelled mNSCs. Longer incubation …
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Human Embryonic Stem Cells - Constitutive Gene Expression and Differentiation Towards Definitive Endoderm and Posterior Foregut Endoderm
… or to introduce fluorescent reporter genes. Constitutive promoters are useful tools due to their high level of expression in most cell types. Different eukaryotic/mammalian and viral constitutive promoters have been reported to ensure high level and sustained activity in hESCs but a …