Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 65 for “"conserved regions"”.
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THE ROLE OF CORONAVIRUS - HOST CELL MEMBRANE INTERACTIONS DURING VIRAL ENTRY AS MEDIATED BY CONSERVED REGIONS OF THE FUSION PEPTIDE
… S, the fusion peptide (FP) is a short, highly conserved domain that inserts into the host membrane, initiating membrane fusion, genome release and subsequent infection. Due to its critical role in viral entry and high sequence conservation across coronaviruses, the FP has been previously …
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HIV subtype C diversity: analysis of the relationship of sequence diversity to proposed epitope locations
… codon sites under positive selection and epitope regions; suggesting epitope regions are evolutionarily conserved. It is possible that epitopes exist in non-conserved regions, yet fail to be detected due to the reference strain diverging from the circulating viral population. To test if CTL …
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Establishment of a transformation procedure to study the role of trypsin inhibitors in soybean
… these two major serine trypsin inhibitors. Conserved regions, which have been identified by searching NCBI and EMBL database, were targeted for down regulation. Seed specific promoters were also isolated to drive the expression of hairpin constructs designed to down-regulate selected …
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The cloning and characterization of an α-amylase and a branching enzyme from Butyrivibrio fibrisolvens H17c and their expression in Escherichia coli
… and other α-amylases in the three highly conserved regions which constitute the active centre. Conserved regions were all located in the N-terminal half of the B. fibrisolvens amylase and no homology to other amylases was detected for the remainder of the protein. Approximately 40% of the …
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CSA-X: Modularized Constrained Multiple Sequence Alignment
… if chosen well, created from the least conserved region of the correct alignments, improves the alignment accuracy. In this study, ProbCons and T-Coffee are used as the underlying MSA programs in CSA-X, and the accuracy of the alignments are measured in terms of Q score and TC score. On …
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Regulation of Brain-Derived Neurotrophic Factor in the Adult Mouse Brain
… of transgenic animals to define BDNF promoter regions; and use of comparative genomics to identify evolutionarily conserved regions of BDNF. The in situ hybridization study suggests that the four distinct BDNF promoters are differentially regulated and that neighboring promoters are …
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Novel use of a cell-penetrating peptide-adaptor system to characterize Akirin constructs and investigate activity of bacterial effector proteins
… and chromatin remodeler Brahma. The functional regions of Akirin are unknown; therefore, TAT-CaM was used to characterize the four conserved regions of Akirin. Bacterial effectors, AopP and AvrA, have been shown to inhibit the NF-kB pathway. Previous studies used infection and transfection …
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The Role of the C-terminus in Bacillus subtilis inorganic pyrophosphatase activity
… a role in multimerization. However, both its conserved and non-conserved regions are absolutely vital for full enzyme activity. The signature sequence is found to be required for both the composition and correct conformation of the active site. Substrate specificity studies suggest the …
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Transcriptome-Wide Characterization of APOBEC1-Catalyzed RNA Editing Events in Macrophages
… events. Additionally, RNA editing in non-coding regions of mRNA transcripts has been shown to influence gene expression in a tissue-specific manner. In mammals, mRNA editing serves a diverse set of biological roles in neuronal function, host defense and lipid metabolism. The major mRNA editors …
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Design, synthesis and evaluation of amphibian isolated peptides as antibacterial and antiproliferative agents
… of the peptides, elimination of the highly conserved regions and modifications of the motif of the peptides such as the C-terminal amidation to study the structure-function relationships. <br/><br/>The results of this study brought some ideas on the targeted modifications of AMPs. These …
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Probing Interactions Between Canonical NOX Domains
… Past research studies in NOXes have identified conserved, putative interacting regions at the interface of the TM and DH domains: the TM B-loop, the TM D-loop, the NADPH-FAD linker, the TM-DH linker, and the C-terminus. Using the SpNox model system, the role of the conserved, putative …
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Molecular Characterization of the Heat Shock-Regulatedgroesl Operon in Lactobacillus Zeae Strain 102S
… with degenerate primers designed to conserved regions and genomic walking were used to determine the sequence of the highly conserved shock response genes groESL (HSP60/10). A rapid RNA isolation procedure was developed, and specific heat induction conditions for the groESL operon …
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Studies on CheA(B): Phosphorylation, methylation and behavioral control during chemotactic signal transduction in Bacillus subtilis
… modulating family. CheA$\sb{\rm B}$ has several conserved regions (including the histidine that is phosphorylated in CheA$\sb{\rm E}$) that coincide with the other autophosphorylated signal transducers. In vitro experiments on partially purified CheA$\sb{\rm B}$ indicates that it also appears to …
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An Efficient Primer Selection Process Combining Progressive and Iterative Multiple Sequence Alignment Strategies: ClustalW and HMMER
… and HMMER alignment tools, is able to identify conserved regions in sequence data in a computationally efficient manner, and from these regions, suggest viable primer candidates. Computational complexity for the HMMER alignment effort has been maintained at O(MN); the suggested process for …
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On the origins of enzyme inhibitor selectivity and promiscuity: a case study of protein kinase binding to staurosporine
… of ligand binding. I find that the most conserved regions are the main chain atoms in the hinge region and I show that the tetrahydropyran ring of staurosporine causes induced-fit of the glycine rich loop. I apply multiple linear regression to select distances measured between the …
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A computational framework for the identification, cataloging, and classification of evolutionary conserved genomic DNA
Evolutionarily conserved genomic regions (ecores) are understudied, and yet comprise a very large percentage of the Human Genome. Highly conserved human-mouse non-coding ecores, for example, are more abundant within the Human Genome than those regions, which are currently estimated to encode for …
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Characterization of Polymorphic Microsatellites in Strawberry and Their Transferability to Other Genera in the Rosaceae Family
… Amelanchier arborea and Potentilla fruticosa had conserved regions resulting in generation of amplicons. For UDF 003, Spirea xbumalda and Prunus persica did not contain a microsatellite although there was some sequence similarity with Fragaria. Size homoplasy, i.e., alleles of identical size with …
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An investigation of the mechanism of PAX7 mediated oncogenesis via in silico and in vitro biology
… a long period of evolutionary time. The highly conserved paired box sequence within the Pax genes encodes a paired DNA binding domain, indicating that the Pax proteins are transcription factors which bind and regulate downstream target genes. Nine Pax genes (Pax1 - Pax9) listed in the National …
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Genetic and genomic studies of mouse and human NR2E1 in cortical disorders, aggressive behaviour, and psychiatric disease
… proximal promoter, and evolutionarily conserved regions of this gene in 60 subjects with microcephaly. Four candidate regulatory mutations were identified. To help interpret these findings, the genomic architecture and molecular evolution of NR2E1 were characterized in …
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Interaction of the Neurospora crassa mitochondrial tyrosyl-tRNA synthetase with group I intron RNAs
… CYT-18 suppressed mutations of phylogenetically conserved nucleotide residues at all positions tested, except for the universally conserved G-residue at the guanosine-binding site. Structure mapping experiments with some selected mutant introns showed that the P7 mutations impaired the formation …
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