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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"connexins"”.
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RegulationofGap Junction Protein,Connexins in Immortalized Human Keratinocytes Cells(HaCaTcells)
… A family of gap junction proteins, the connexins(Cx), has been identified and comprises at least 20 members. Middle ear cholesteatoma is characterized by the presence of a squamous epithelium invading in the middle ear cavity, and is one of the most prevalent forms of otological …
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Electrical synapses: cellular morphology and identification of connexins in the mouse retina
Das Ziel meiner Dissertation war die Zahl der deplatzierten Amakrinzelltypen (DAZ), die Identifikation der Cx45-exprimierenden Amakrinzellen (AZ) und Cx30.2-exprimierenden Ganglienzellen (GZ) zu erforschen. Außerdem wurde die Lokalisation von Cx57 in den Horizontalzellen (HZ) untersucht. Gefunden …
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Identification of Connexins in Facilitating Neuron-Satellite Glial Cell Signaling in Trigeminal Ganglia
… was to identify the gap junction proteins, or connexins (Cxs), in the ganglion in response to three different models of inflammation: acute, acute-sensitization, and chronic. Initially, quantitative PCR was used to demonstrate that mRNA for Cxs: 26, 29, 36, 37, 40, 43, 50, and 57 was expressed …
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Regulation of specific connexins differentially alters gap junction permeability and endothelial cell function
… or peptide inhibitors to downregulate specific connexins we examined the role of gap junctions in intercellular diffusion, calcium excitation, and in mediating the expression of vascular regulators transforming growth factor-[Beta][ (TGF-[beta]), prostacyclin, and endothelial nitric oxide …
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Function Study of Atrial Fibrillation-Linked Connexin Mutants and Heterotypic Docking Compatibility of Cardiac Connexins
Gap junctions are critical for the propagation of action potentials in the heart. Many autosomal dominant Cx40 mutations have been linked to atrial fibrillation (AF) and have showed GJ impairments. However, others such as L221I and V85I showed apparently no GJ impairments. Cells expressing L221I or …
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Turnover Rate of The Neuronal Connexin Cx36 In Hela Cells
… turnover rates of Cx36 have not been studied. Connexins have relatively fast turnover rates, with short half-lives measured to be 1.5 to 3.5 hours in pulse-chase analyses of connexins (Cx26 and Cx43) in tissue culture cells and whole organs. We utilized HaloTag technology to study the turnover …
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Putative Pore-lining Residues and Intracellular Magnesium Influence Connexin50 Unitary Gap Junction Channel Conductance
Gap junction channels (GJs) are oligomers of connexins forming channels linking neighbouring cells. GJs formed by different connexins show distinct unitary channel conductance (γj), transjunctional-voltage dependent gating (Vj-gating) properties, and magnesium modulation. We investigate here how …
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Functions of connexin 46 in lens and solid tumors during hypoxia
… are many types and families of protein pores, connexins comprise a unique family. Six connexin monomers assemble into a hemichannel, which is transported to the cell membrane. An opposing cell membrane containing compatible connexin hemichannels is located and connected, forming an …
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C-terminal lysines modulate Connexin32 turnover and its ability to suppress growth of Neuro-2a cell cultures
… In addition to their function in GJ, connexins also regulate a variety of physiological processes by forming hemichannels that are involved in paracrine signaling (Sanchez, Orellana et al. 2009) and through interactions with other proteins in the cytoplasm(Francis, Xu et al. 2011) and …
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The Characterization of Cx31 in Keratinocytes and its Link to Erythrokeratodermia Variabilis et Progressiva
… Cx31 exhibits a longer half-life than most connexins and that the L209F and L135V mutants cause EKVP through mechanisms that include the loss of Cx31 gap junction intercellular communication and keratinocyte death.
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Cardiovascular Characterization of Cx40/Panx1 Single and Double Knockout Mice
Connexins (Cxs) and pannexins (Panxs) are protein families that form large-pore channels which exist at the plasma membrane for both intracellular and extracellular signaling. Given their potential for overlapping cellular signaling functions we proposed that mice lacking both a connexin and a …
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Mutations in Connexin43 and Connexin40 Associated with Human Atrial Fibrillation; Physiology and Mechanism
… three novel Cx40 mutants, fluorescently tagged connexins were visualized in HeLa cells using confocal microscopy. Electrophysiological studies were performed by a member of our lab to test the function of mutant gap junction channels. The Cx43 frameshift mutant (Cx43-932delC) caused …
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Functional study of Gap Junctional Intercellular Communication in Human Keratinocyte Cell Line
… among almost all tissues, are composed of connexins in vertebrate. The essential role of gap junction-mediated intercellular communications(GJIC) for coordination of cell homeostasis, tissue functions and organ development are well reported by many researches using genetics and mutant …
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Modulation of the vascular function by cannabinoids, calcitonin gene related peptide and connexin hemichannels
… with some delay also GJs; it targets multiple connexins because it mimics well-conserved extracellular domains of the connexin protein. By contrast, TAT-L2 peptide has specificity at two levels: it only inhibits HCs but not GJs and it has specificity for Cx43 and not for other connexins. The …
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Two-Color Fluorescent Analysis of Connexin 36 Turnover and Trafficking – Relationship to Functional Plasticity
… throughout existing gap junctions. Existing connexins also diffused into photobleached areas with a half-time of less than 2 seconds. Recovery of connexin was impaired when laser power was focused and phototoxicity may be responsible. To better understand mechanisms of turnover we studied the …
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Identificaiton of the pore-lining helices of connexin 43 gap-junctional hemichannels
… adjacent cells. The hemichannels are hexamers of connexins, proteins that have four transmembrane helices. The transmembrane helices that line the gap-junctional pore have not been identified, and their identification was the main goal of my dissertation project. To accomplish this goal, I used a …
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Effects of constitutive and acute Connexin 36 deficiency on brain-wide susceptibility to PTZ-induced neuronal hyperactivity
Connexins are transmembrane proteins that form hemichannels allowing the exchange of molecules between the extracellular space and the cell interior. Two hemichannels from adjacent cells dock and form a continuous gap junction pore, thereby permitting direct intercellular communication. Connexin 36 …
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Effects of connexin modulators in animal models of retinal degeneration: delivery and treatment efficacy
… injury. Therefore the role of pannexins and connexins in injury of the retina in this animal model needed further experimental evidence. Thus, the aim of this thesis was to determine the effect of hemichannel blockers (Cx43 mimetic peptides, tonabersat) and pannexin channel-blockers …
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Signaling Induced by Inflammatory Mediators in the Rodent Pulmonary Microvasculature
… communication via gap junctions composed of connexins, such as Connexin 43 (Cx43). Herein, we explored how intercellular communication through Cx43-containing gap junctions mediates thrombin-induced signaling as well as the interplay between thrombin- and S1P- induced signaling on the …
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The Role of Connexin43 and Gap Junctional Intercellular Communication in the Apoptotic Process of Auditory Cells
… organ of Cortis are major targets, where many connexins (Cxs) are expressed. Cxs are a family of membrane proteins and these are expressed abundantly in mammalian inner ears. Cxs oligomerize into a hexameric connexon, also known as a hemichannel, and transport to plasma membranes along …
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