Global ETD Search
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Showing 1 to 19 of 19 for “"conjugative transposon"”.
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Excision of the Bacteroides Conjugative Transposon CTnDOT
In this study, we showed that expression of the exc operon is regulated by tetracycline and mediated by the regulatory proteins, RteA, RteB, and RteC. Tetracycline induces the expression of rteA and rteB, genes in the tetQ operon. Then, RteB induces the expression of rteC. RteC, subsequently, …
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Regulatory Genes of the Bacteroides Conjugative Transposon, CTnDOT
In order to examine which genes are responsible for excision, I first demonstrated that the excision genes orf2c, orf2d, orf3, and exc are part of an operon (the orf2c operon) and that this operon is regulated at the transcriptional level. I found that RteC is an DNA-binding activator of the orf2c …
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The Integration and Excision Mechanisms of the Bacteroides Conjugative Transposon CTnDOT
A critical requirement for studying transposition is the availability of a defined in vitro transposition system. Using the partial-purified CTnDOT Int protein, an in vitro integration system was developed and a host factor(s) from Bacteroides cell extract was shown to be important for integration. …
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Transcriptional regulation of mobilization and transfer of the Bacteroides conjugative transposon CTnDOT
… mobile genetic elements. One such element is a conjugative transposon (sometimes referred to as an integrative conjugative element, or ICE) called CTnDOT, which is a 65 kb ICE that encodes resistance to the antibiotics erythromycin and tetracycline. A notable feature of CTnDOT is that excision …
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Tetracycline-Associated Regulation of the Transfer Genes of the Bacteroides Conjugative Transposon CTnDOT
Many human colonic Bacteroides spp. harbor a conjugative transposon (CTn) called CTnDOT. CTnDOT carries two antibiotic resistance genes tetQ and ermF. A distinctive feature of CTnDOT is that both its excision and transfer are stimulated by tetracycline. The regulation of excision has been described …
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Construction and Use of a System for Studying the Integration of Bacteroides Conjugative Transposons
… is known about the way in which Bacteroides conjugative transposons integrate into their chromosomal target sites, or what the nature of these sites are. In the past, this question has been very difficult to address due to the large size of the Bacteroides conjugative transposons. In this …
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The DNA Transfer Region of Bacteroides Conjugative Transposons Tc(r)Em(r)DOT and Tc(r)ERL
… study of the conjugal transfer genes found on a conjugative transposon. The complete sequence of a functional transfer region is presented and comparative analysis is made between the transfer gene sequences of several conjugative transposons. Two of the newly discovered transfer genes possess …
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The Role of Bacteroides Conjugative Transposons in the Spread of Antibiotic Resistance Genes, And, The Role of recA in DNA Damage Repair in Deincoccus Radiodurans
… segment of DNA, located on a Bacteroides conjugative transposon (CTnBST), that carries a gene for erythromycin resistance (ermB). The same DNA sequence (100% identical) had been previously identified in the genome of Arcanobacterium pyogenes, a gram positive opportunistic pathogen that is …
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Positive regulators of Bacteroides CTnDOT excision and transfer
… both excision and transfer of a Bacteroides Conjugative transposon CTnDOT. It was shown previously that a gene, rteC, is necessary for tetracycline-stimulated transcriptional regulation of the xis2c operon, which contains the excision genes. The protein encoded by this gene, RteC, did not …
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Role of the core in CTnDOT integration
… tyrosine recombinase encoded by the Bacteroides conjugative transposon CTnDOT. It catalyzes integration and excision into and out of the bacterial host chromosome. Although it is a member of the well studied tyrosine recombinase family, IntDOT is of particular interest because it can catalyze …
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Studies on Clostridium acetobutylicum P262 : sporulation induction and analysis of an oligosporogenous, solvent-deficient mutant
… to be 75·C. acetobutylicum mutants m5 06 were by transposon mutagenesis using the conjugative transposon Tn925::Tn917, not transposon Tn925 as previously thought [Babb. B.L. 1990. B.Sc. (Honours) thesis, University of Cape Town. South Africa]. The spore development and the fermentation profiles of …
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Activities of Bacteroides Conjugative Transposons: CTnGERM1 and CTnDOT
CTnDOT is a conjugative transposon (CTn) that is found in many Bacteroides strains. Transfer of CTnDOT is stimulated 100--1000 fold if the cells are exposed to tetracycline (Tc). An operon that contains a Tc resistance gene, tetQ, and two regulatory genes, rteA and rteB, is essential for control of …
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Regulation of the mobile genetic element ICEBs1 by a conserved repressor and anti-repressor
… genetic element ICEBs1 is an integrative and conjugative element (a conjugative transposon) found in the Bacillus subtilis chromosome. The SOS response and the RapI-Phrl sensory system activate ICEBsl gene expression, excision, and transfer by inactivating the ICEBsl repressor protein ImmR. …
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Characterization of TraM, a Required Bacteroides Fragilis Conjugal Apparatus Protein That Interacts with the B. Fragilis Putative Coupling Protein Bcta and the B. Fragilis Relaxase Protein BmpH
… clinical isolate LV23 harbors a 37kb conjugative transposon, BTF-37. Two important conjugal apparatus proteins encoded by BTF-37 are BctA, a predicted coupling protein (CP) that powers DNA translocation, and ORF7 (TraM). In this study, we demonstrated that TraM exhibits characteristics …
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The development of tools to allow genetic and genomic analysis of Frankia
… This was achieved through the use of a conjugative transposon, Tn916, which was introduced into Frankia isolate Eul1c by mating with Enterococcus faecalis GC110. The generation of stable tetracycline and novobiocin resistant transconjugants suggests that the transposon inserted into the …
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Characterization of IntDOT, the CTnDOT Integrase
… revealed that the integrase of the Bacteroides conjugative transposon CTnDOT (IntDOT) might be a member of the tyrosine recombinase family because IntDOT has five of six highly conserved residues found in the catalytic domains of tyrosine recombinases. Yet, IntDOT catalyzes a reaction that …
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Protein/DNA interactions during site-specific recombination
… carry large, integrated elements including conjugative transposons (CTns) and mobilizable transposons (MTns). One such conjugative transposon is CTnDOT, which is 65 kb in size and encodes resistances to tetracycline and erythromycin. In recent years, CTnDOT has been implicated in the spread …
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The role of Bacteroides Host Factor A in CTnDOT integration and Holliday junction resolution
… recombination. It is an example of a Conjugative Transposon (CTn). These elements were later classified as Integrated Conjugative Elements (ICEs). They frequently carry resistance to antibiotics and always contain the genes to mediate their own transfer by conjugation. CTnDOT is 65 kb …
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Structure-function analysis of IntDOT, the CTnDOT integrase
… movement of CTnDOT that is an integrative conjugative element (ICE) found in Bacteroides spp. IntDOT has been classified in the tyrosine recombinase family. However, unlike most other tyrosine recombinases that require sequence identity in the region where strand exchanges occur, IntDOT …