Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"conjugative plasmid"”.
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Characterization of two prevalent yet distinct conjugation systems among lactococci
… products with diverse organoleptic attributes. Plasmids present in L. lactis and L. cremoris species harbor genes associated with many important technological traits, such as bacteriophage resistance, lactose metabolism and exopolysaccharide (EPS) production. There are practical limitations …
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Allele dynamics in conjugative plasmids: Horizontal transfer promotes allele segregation in multicopy plasmids
… bacteria pose a major threat to human health. Plasmids, particularly through horizontal transfer, are considered key drivers of antimicrobial resistance spread because they can disseminate resistance alleles between bacterial lineages and, in some cases, even across species boundaries. As …
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Factors that affect horizontal gene transfer in enteric bacteria
… enhanced conjugation efficiencies of a conjugative plasmid from a clinical strain of Salmonella Typhimurium to an E. coli recipient in vitro. Additional experiments determined the influence of the antimicrobial concentrations above, equal to and below the minimum inhibitory concentration …
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Analysis of catabolic plasmids in pseudomonas
… Pseudomonas strain Mr74 harbours the single TOt plasmid pWW74, and this plasmid carries two related genes for catechol 2,3-oxygenase (C230) enzymes. One of these genes had been previously cloned on a 2.05 Kb XhoI DNA fragment to yield the recombinant plasmid pWW74-3016. Internal regions of this …
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Understanding Type A Clostridium perfringens in Foal Necrotizing Enteritis and Canine Acute Hemorrhagic Diarrhea
… same pathogenicity locus (NetF locus) on a tcp-conjugative plasmid and that NetG was encoded by a second plasmid (pNetG) on a unique pathogenicity locus (NetG locus). In addition, these two isolates shared three plasmids, including the netF/netE plasmid (pNetF), a cpe/cpb2 plasmid (pCPE), and a …
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Development of Chimeric Type Iv Secretion Systems For Transfer of Heterologous Substrates Across The Gram-Negative Cell Envelope
… to demonstrate transfer of the promiscuous IncQ plasmid pML122 or known <em>A. tumefaciens</em> effector proteins. I next tested the <em>E. coli</em> pKM101-encoded T4SS, which is known to transfer DNA substrates, for the capacity to deliver heterologous protein substrates to <em>E. coli</em> …
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Single cell analysis of bacterial communication and gene transfer by Enterococcus faecalis
… resistance by mechanisms like conjugation and conjugative transfer of plasmids can occur at a high frequency without antibiotic selection. Conjugative plasmid pCF10 encodes tetracycline resistance and transfer between E. faecalis cells is facilitated by cell-to-cell communication. This …
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Engineering Escherichia coli as a general genome synthesis platform
… workflows. However, delivery of large helper plasmids encoding the conjugative function is required to convert E. coli strains to conjugation-competent donor strains. I present orthogonal conjugation, a method that leverages conjugative plasmid orthogonality to enable the efficient and simple …