Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 32 for “"congenic"”.
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Genetic Loci Contributing to Spontaneous Autoimmune Diabetes
… 34Mb of F344 between D4Rat253 and D4Rhw6 in the congenic DR.lyp rat line with two Mb of BBDP DNA, encompassing the Gimap5 mutation, introgressed on the DR genetic background. The aim of this thesis was to characterize the F344 DNA introgression, test the hypothesis that the introgression would …
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Of mice, genes, and radiation: the genetics of non-hereditary breast cancer explored using the common laboratory BALB/c mouse
… of the DNA repair gene Prkdc (DNA-PKcs). Strains congenic for the common (PrkdcB6) and variant (Prkdc BALB) alleles of Prkdc are developed, genotypically validated, and used to examine the functional consequences of Prkdc BALB and its linkage with radiation susceptibility. DNA-PKcs protein …
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Haplotype-Specific Effects of the Slam/Cd2 Family on the Immune Response
… model of systemic lupus erythematosus (SLE). Congenic B6 mice carrying the Sle1b locus produce anti-nuclear autoantibodies (ANAs) but do not develop lupus nephritis as seen in the parental NZM2410 strain. Fine mapping of the Sle1b locus placed it within a 900kb interval between 171.3 and …
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Characterization of 𝘈𝘵𝘩𝘴𝘲1, an Atherosclerosis Modifier Locus on Mouse Chromosome 4:
… studies in humans. In this study we have used congenic mice that were previously constructed to isolate 𝘈𝘵𝘩𝘴𝘲1, an atherosclerosis susceptibility locus identified in a cross between MOLF/Ei and C57BL/6J-𝘓𝘥𝘭𝘳⁻⸍⁻. Atherosclerosis studies performed in congenic mice carrying the MOLF-derived …
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Associations of egg production with the major histocompatibility complex in broiler breeder hens
… determine if the MHC affects reproduction of non-congenic broiler breeder hens. Genomic DNA was isolated from 35 hens. Restriction fragment length polymorphism (RFLP) analysis was performed on each sample, using a chicken class II MHC probe (Xu et al. 1989). Restriction endonuclease digestion …
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Genetic Exploration of Hereditary Immune Diseases
… from DBA/1-/- and BALB/c-/- mice. We created a congenic strain carrying the DBA/1-/- region of chromosome 1 that contains the QTL on the BALB/c-/- background. We also created a congenic strain carrying the BALB/c-/- region of chromosome 1 that contains the QTL on the DBA/1-/- background. We then …
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Defining How Polymorphisms at the Slam Family Locus Affect NK and T Cell Function
… haplotype of the SF, and B6.Sle1b mice that are congenic for the 900 kb interval surrounding the z haplotype of the SF. Thus, differences in lymphocyte function can be directly attributed to polymorphism at the SF locus. These congenic mouse strains were used in two studies: 1) to characterize …
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Genetic Control of Experimentally Induced Reproductive Autoimmune Phenotypes
… resistant and susceptible strains. Multiple congenic strains of mice encompassing Aod1 were constructed and demonstrated that Aod1 primarily influences susceptibility to oophoritis and comprises two regions: Aod1a on telomeric chromosome 16 that engenders susceptibility, and the more …
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Functional genomics in the stroke-prone spontaneously hypertensive rat: genome wide and candidate gene analysis
… normotensive Wistar Kyoto (WKY) and chromosome 2 congenic rats identified differential expression of several glutathione cycling genes. The Gstm1 promoter was investigated by luciferase and Transfac bioinformatic analysis, implicating two polymorphism clusters and several transcription factors in …
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Narrowing of the SLES1 Internal Reveals Complex Epistatic Interactions in the Suppression of Autoimmunity
… loss in tolerance mediated by the B6.Sle1 congenic. Sles1 had previously been fine-mapped to a remarkably gene-rich region on murine chromosome 17 containing nearly 70 genes. A series of mouse strains were constructed with a variety of suppressive and non-suppressive variants of Sles1 on …
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Pathological and genetic analysis of host susceptibility to cardiovirulent coxsackievirus B3 infection in mice
… Three inbred strains of mice and two congenic strains were infected with CVB3. Differences in survival, body weight loss, quantification of myocarditis and quantification of sarcolemmal disruption were found by comparing three sets of mice sharing the same H-2 haplotype but not the …
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Involvement of the lysosomal cysteine peptidase cathepsin B in tumour progression and formation of metastases
… a mixed C57BL/6J and 129SvJ background were made congenic for the FVB/N strain. FVB/N-TgN(MMTVPyVT)-CTSB, wt, ht and ko were generated through inter-crossing (PyCB strain). The mice were sacrificed sixty days after the discovery of the first palpable mammary tumour. The weight of primary tumours …
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Identification and Characterization of APOBEC1 mRNA Editing Targets: A Transcriptomics Approach
… bioinformatic analysis to compare wild-type and congenic apobec1-/- transcriptomes for specific single nucleotide variants indicative of editing. Following technical validation, the screening approach was used to identify more than 30 previously undescribed APOBEC1 editing sites in enterocyte …
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An investigation of the regulation of Sialoadhesin ligands on CD4+ T cells and its potential significance in Systemic Lupus Erythematosus
… on disease severity. Secondly, using speed congenic method, BWF1 mice deficient for Sn were generated. Data presented in this thesis suggest that Sn might play an antiinflammatory role in murine SLE.
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Delineating the Role of CD244 in NK Cell Cytotoxicity and the Contribution of Ly108 to Thymocyte Development
… pathologies. For these studies, we used mice congenic at the SLAM family locus (B6 –b haplotype and B6.Sle1b–z haplotype) to study two major questions: 1.) how do polymorphisms in CD244 affect NK function? And 2.) how do polymorphisms in Ly108 affect T cell tolerance? In the studies presented …
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The Role of the Complement System in Mousepox Infection
… and generated higher peak titers compared to the congenic controls. Specifically, increased hepatic inflammation and necrosis correlated with these higher tissue titers and likely contributed to the morbidity in the C3<super>-/-</super> mice. In vitro, the complement system in na├»ve C57BL/6 mouse …
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Role of Properdin in Tumour Growth and Cell Recruitment
… from<br/>wildtype mice, macrophages from congenic properdin deficient mice showed<br/>skewing towards M2 profile, encompassing mRNA expression for genes involved<br/>in arginine metabolism, production of type 2 cytokines, and relatively lower<br/>surface expression of molecules needed for …
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Mitochondrial and chromosomal genomics in type 2 diabetes
… loci, Niddm71, was dissected further using a congenic rat, C9B. Characterization of the C9B rat revealed that the Niddm71 locus encodes for a defect in insulin secretion and a defect in mitochondrial function. With a combination of bioinformatics and experimental methods we identified several …
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Esx1 Dosage Impacts Reproductive Fitness: Effects On Viability and Fertility
… development and fetal growth. We show C57Bl6-congenic Esx1 mutants display a more severe phenotype with decreased viability and that the 129 genetic background contains dominant modifier genes that enhance Esx1 mutant survival. Varying Esx1 dosage impacts testicular germ cell development. Esx1 …
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Mouse modifiers of intestinal tumorigenesis
… clone Mom1, Gould et al created a B6.Mom1 AKR congenic strain isolating Mom1 from other AKR resistance factors. Separated from other loci, a single copy of Mom1 AKR reduced tumor number by 50% and two copies produced a 70% reduction. We have used recombinant lines derived from B6.Mom1 AKR to …
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