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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 367 for “"conformational changes"”.
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Mesoscopic modeling of protein conformational changes
"The conformational changes of proteins are studied theoretically with the help of coarsegrained mesoscopic models of protein structure. The models explicitly incorporate the effects of the polarity of the peptide backbone and of the specificity of hydrophobic interactions. These two features are …
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Agonist-Induced Conformational Changes In The Nmda Receptor
… and glutamate molecules and undergo a series of conformational changes that results in the opening of a cation-permeable pore in the neuronal plasma membrane. Various aspects of NMDA receptor function are not fully understood, including the phenomenon of negative cooperativity between the …
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Gene Regulation Through Artificially Induced DNA Conformational Changes
The conformation of DNA has been shown to play an important role in the regulation of gene expression. A consequence of this finding is that agents that alter the conformation of DNA should also affect the regulation of gene expression. To explore this, we used tethered-triple helix …
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Conformational Changes In The Extracellular Domain of Glutamate Receptors
… the unique advantage of tracking large scale conformational changes associated with receptor activation and desensitization. In this dissertation, LRET, in combination with biochemical and electrophysiological studies, were performed on the NMDA receptors to draw a correlation between …
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Fluorescent Tools for Watching Conformational Changes of Biological Molecules
… assay to study the timing of the neck linker conformational change that occurs as kinesin walks processively along a microtubule. We observed repetitive, alternating cycles of fluorescence and quenching as individual kinesin motors walked along microtubules. By monitoring the position of the …
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Investigating Ligand Induced Conformational Changes in the Estrogen Receptor
Primarily, we have investigated ER conformational structure using the reporter group method of site-directed spin labeling (SDSL), along with electron paramagnetic resonance (EPR) spectroscopy. In this work, B and C parameter calculations from EPR line-shape theory express receptor differences …
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Characterization of Ligand-Induced Conformational Changes in the EGF Receptor
… DISSERTATION Characterization of ligand-induced conformational changes in the EGF receptor by Katherine S. Yang Doctor of Philosophy in Biochemistry Washington University in St. Louis, 2009 Professor Linda Pike, Chairperson The epidermal growth factor: EGF) receptor is a classical receptor …
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Markov state modeling of binding and conformational changes of proteins
… other molecules. However, the interplay between conformational change and binding is not fully understood. In this work, this interplay is investigated with molecular dynamics (MD) simulations of the protein-peptide system Mdm2-PMI and by analysis of data from relaxation experiments. The central …
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Single-molecule visualization of conformational changes in the SecA ATPase
… is contradictory. We aim to directly measure conformational changes of the 2HF by utilizing single-molecule Fbrster resonance energy transfer (smFRET). Directly measuring conformational changes in an ATPase will also provide further insight into the guiding principles of ATPase function. …
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Gold and Magnetite Nanoparticles as Sensors of Conformational Changes in Proteins
… and influence of different mechanical factors, conformational changes in the polypeptide occurred. The aggregation of the gold nanoparticles related to the folding of the polypeptide caused shifts in color of the solutions from red to blue that were measured by UV-Vis spectrometry. Different …
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Cationic Phenylene Ethynylene Polymers and Oligomers Induce Conformational Changes to Proteins
… The compound also showed morphological changes to the viral capsids composed of protein assemblies. Due to the results of previous research the mechanism by which the composed interacted with the protein remained unanswered. Studies were conducted using two model proteins BSA and …
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Investigation of the dynamics and conformational changes of the U1A protein
The U1A-SL2 RNA complex is a model system for studying interactions between RNA and the RNA recognition motif (RRM), which is one of the most common RNA binding domains. Despite extensive studies conducted to study the origins of U1A-RNA affinity and specificity, little is known about the kinetics …
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Probing the conformational changes of the yeast mitochondrial ADP/ATP carrier
… ADP and exports ATP by switching between two conformational states. In the cytoplasmic state, which can be locked by carboxy-atractyloside, the substrate binding site is accessible to the cytoplasm, whereas in the matrix state, which can be locked by bongkrekic acid, the substrate binding site …
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Mechanistic investigation of coordinated conformational changes in multisubunit ion channels and enzymes
… distinct functional components. Coordinated conformational changes through allosteric interactions between subunits and/or between functional units can efficiently regulate protein activity. This dissertation describes investigations of coordinated conformational changes in two systems: the …
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Functional Consequences of Rna Exosome Complex Alteration By Conformational Changes and Cofactor Binding
<p>The RNA exosome is an essential 3’-5 ribonuclease that processes or degrades a variety of RNA species in eukaryotes. It is composed of nine structural cores and one catalytic subunit, Rrp44. Structural studies captured two different conformations of Rrp44, Rrp44<sup>ch</sup> (channel) and …
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Conformational changes in the (Ca²⁺, Mg²⁺)-ATPase of sarcoplasmic reticulum during energy transduction
… Mg²⁺)-ATPase activity is unimpaired. Possible conformational changes associated with this uncoupling were monitored by following alterations in kinetic mobility of sulphydryl (-SH) groups either by using 5, 5'-dithiobis- (2-nitrobenzoate) (DTNB) and stopped flow analysis or …
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NMR investigations of blood coagulation: Conformational changes of membrane bilayers and proteins in blood coagulation
… employ solution and solid-state NMR to observe changes in dynamics and conformation of both membranes and membrane associated proteins upon interaction with other biological molecules. Initial studies focus on the interaction of neurological proteins alpha-synuclein and endosulfine-alpha. Using …
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Identification and characterization of low pH-triggered conformational changes in the herpes simplex virus glycoprotein B
… that mildly acidic low pH triggers specific conformational changes in HSV gB at a pH of 5.7 to 6.0. The antigenic structure of gB functional region I that is critical for fusion is specifically altered by mildly acidic pH both in vitro and during entry into host cells. Point mutations within …
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Theoretical Approaches to the Characterization of Water, Aqueous Interfaces, and Improved Sampling of Protein Conformational Changes
<p>Methods to advance the understanding of water and other aqueous systems are devel- oped. This work falls into three areas: The creation of better interaction potentials for water, improved methods for sampling configurational space, and the applications of these methods to understand systems of …
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