Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 248 for “"conformational change"”.
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Electrostatics and packing in biomolecules : accounting for conformational change in protein folding and binding
The role of electrostatics and packing in protein folding and molecular association was assessed in different biomolecular systems. A continuum electrostatic model was applied to long-range electrostatic effects in the binding of human carbonic anhydrase II to a sulfonamide inhibitor. The effect of …
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Anatomy of the coiled coil and its role in the conformational change of influenza hemagglutinin
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1995.
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In Search of Catalytic Proficiency: The Importance of Enzyme Conformational Change to Orotidine 5'-Monophosphate Decarboxylase Catalysis
… OMPDC. Increasingly in enzymology, the role of conformational change in enzyme catalysis has been recognized as an important one. This research has shed light on the conformational changes that take place when substrate binds OMPDC and how the two events are coordinated.
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In search of catalytic proficiency: The importance of enzyme conformational change to orotidine 5’-monophosphate decarboxylase catalysis
The focus of this research is the role of conformational flexibility in catalysis by a TIM-barrel enzyme in pyrimidine biosynthesis, orotidine 5’-monophosphate decarboxylase (OMPDC). OMPDC catalyzes the decarboxylation of OMP to UMP; the uncatalyzed rate for this reaction has been estimated to be …
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The molecular basis of nucleotide recognition for T7 DNA polymerase
… DNA polymerases suggested that a rate-limiting conformational change preceding chemistry accounts for the high specificity following the induced fit mechanism. However, the identity of this rate-limiting conformational change and how it contributes to the fidelity is still under debate. An …
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Markov state modeling of binding and conformational changes of proteins
… of functions in organisms. To this end, they change their conformation and bind to other molecules. However, the interplay between conformational change and binding is not fully understood. In this work, this interplay is investigated with molecular dynamics (MD) simulations of the …
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Fast Processes in Heme Proteins: Mechanisms of Electron Transfer and Carbon Monoxide Recombination
… BM{-}3}$ has shown that this enzyme undergoes a conformational change upon substrate binding. The dynamics of the active site are probed through analysis of the geminate recombination in the presence and absence of substrate.
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Application of hydrogen deuterium exchange mass spectrometry in protein-ligand and protein-protein interactions
… of MS based techniques. Hydrogen-deuterium exchange mass spectrometry (HDX-MS), a combination of HPLC and MS, has become a common and sensitive tool to probe protein structural flexibility and solution dynamics. In this dissertation, HDX-MS was applied to study dynamic changes of proteins due …
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Kinetic and Mechanistic Characterization of the Uracil Phosphoribosyltransferase Activity of Bacillus Subtilis PyrR
… was consistent with each of the four proposed conformational change models.
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Biochemical mechanisms for selectivity governing RNA and DNA replication
… about the controversial nucleotide induced conformational change. I developed methods to directly measure the conformational change by site specifically incorporating a fluorescent unnatural amino acid into the enzyme at a position that gives a fluorescence signal upon nucleotide binding. In …
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Mechanism and consequences of Mu transpososome remodeling by the ClpX chaperone
… transposase monomers, and that it exerts a conformational change in transposase subunits during remodeling. However, only subunits directly contacted by ClpX undergo a conformational change. Analysis of the minimal interaction necessary between ClpX and the complex revealed that recognition …
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Structural characterization of superbug proteins involved in regulating beta-lactam resistance
… by β-lactams is not accompanied by a BlaRs conformational change. It is also shown that mutation of the BlaR1 L2 loop prevents induction of β-lactamase expression in vivo, supporting that the L2 loop plays an important role in signal transduction. The intrinsic resistance of Pseudomonas …
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The membrane lipid composition regulates talin mediated integrin activation
… and transmembrane domain and significant conformational changes in the extracellular domains, resulting in a dramatic increase in integrin's affinity for ligands. Membrane bilayers containing anionic lipids are indispensable for proper talin-integrin interaction, yet the detail picture of …
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Trans-2-aminocyclohexanol as a pH-sensitive conformation switch in liposomes
… hydrogen bond with the neighboring -OH. This change will force the bonds switch to from equatorial conformation to axial conformation. This conformational change is transmitted by the structure of the molecular, and induces consequently dramatic conformational change of the two long lipid …
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RNA replication by poliovirus RNA-dependent RNA polymerase: effects of residue 5 on elongation complex stability and processivity
… the primer/template RNA substrate and undergo a conformational change to form a stable RNA-polymerase complex. After the stable RNA-polymerase complex is formed the polymerase undergoes a second conformational change associated with the addition of the first nucleotide to the primer. It is only …
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Contribution à l’étude des peptides obliques impliqués dans des transconformations
… proteins is important to understand the transconformational processes. These proteins have a unique common feature : they undergo a conformational change leading to the formation of highly structured fibrils that are mainly β conformed. These proteins are difficult to study experimentally due …
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Fluorescent Tools for Watching Conformational Changes of Biological Molecules
… assay to study the timing of the neck linker conformational change that occurs as kinesin walks processively along a microtubule. We observed repetitive, alternating cycles of fluorescence and quenching as individual kinesin motors walked along microtubules. By monitoring the position of the …
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Mechanotransduction by talin : a molecular dynamics study of force-induced recruitment of vinculin to a focal adhesion complex
… (MD) is employed to demonstrate a force-induced conformational change that exposes the cryptic vinculin-binding-residues of VBS1 to solvent under applied forces along a realistic pulling direction. VBS 1 undergoes a rigid body rotation by an applied torque transmitted through hydrogen-bonds and …
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