Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 76 for “"confocal laser scanning microscopy"”.
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Confocal laser scanning microscopy of nanoparticles applied to immunosorbent assays
… of this project was to demonstrate and develop a confocal readout method for fluorescent immunosorbent assays and investigate its potential advantages in comparison to traditional immunoassays. The key point of a confocal immunosorbent assay is the ability to detect the thin layer of immunoassay …
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Three-Dimensional Volume Reconstruction From Fluorescent Confocal Laser Scanning Microscopy Imagery
The broader impact of my work is in providing the algorithms in a form of web-enabled tools to the medical community so that medical researchers can minimize laborious and time intensive 3D volume reconstructions using the tools and computational resources at NCSA.
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Confocal Laser Scanning Microscopy As A Tool For The Investigation Of Tetracycline Fluorescence In Archaeologicalhuman Bone
… of remains postmortem. Standard epifluorescence microscopy is the most common tool employed in the analysis of these labels. Though valuable, this technique is limited by its inability to penetrate bone three-dimensionally and its inclusion of out-of-focus light, possibly disrupting accurate …
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Test Development and Material Characterization of Hot Poured Crack Sealant at Low Temperature
… of the sealants were also investigated by confocal laser scanning microscopy (CLSM), environmental scanning electron microscopy (ESEM), gel-permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), and thermo-gravimetric analysis (TGA). These techniques reveal the …
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Novel Endodontic Disinfection Approach Using Nanotechnology
… with currently used chemical modalities using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) as analytical tools. We hypothesized that iron oxide NP with enzyme-like (peroxidase) activity catalyzes H2O2 to promote bacterial killing within dentinal tubules (DT) …
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Multiplexed nanoparticle-based immunoassays
… fluorophores and nanoparticles. Epi-fluorescence microscopy, confocal laser scanning microscopy and programmable array microscopy were used to detect signals from mixtures of conventional organic fluorophores, quantum dots and silica nanoparticles doped with europium, samarium and terbium in …
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Microbiological Study of Bacterial Load Reduction in the Root Canal System Utilizing a Novel ErYagLaser
… to evaluate the effectiveness of a novel Er:Yag laser-activated irrigation technique in reducing bacterial load in root canals. In this study, mandibular molars are prepared and cultured with Enterococcus faecalis, and experiment was perform to test whether laser-activated irrigation could …
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Design, fabrication and testing of an acoustic resonator-based biosensor for the detection of cancer biomarkers
… assessed using water contact angle, atomic force microscopy and confocal laser scanning microscopy. Functionalized biosensors are then used to quantify the concentration of known proteins marker in both a purified medium and a physiologically-relevant medium.
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Material, acoustic, and mechanical properties of mosquito and midge antennae
… of antennal hearing in insects. To this end, Confocal Laser Scanning Microscopy (CLSM), Finite Element Modelling (FEM) and Laser Doppler Vibrometry (LDV) were employed. The combination of the first two allows, in absence of prior knowledge of the material properties of mosquito or midge …
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Glutathione Dynamics in Arabidopsis Seed Development and Germination
… Cytosolic glutathione was measured in situ by confocal laser scanning microscopy. Stress in the form of natural and accelerated ageing, and germination at high and low temperature and at low water potential was applied to both WT and anGR2 seeds to test vigor. Results show similar glutathione …
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Nanoscale surface modification of wood veneers for adhesion
… ionic strength for polyelectrolyte adsorption. Confocal laser scanning microscopy (CLSM) and Environmental Scanning Electron Microscope (ESEM) were used to characterize adsorbed LbL multilayers of poly(acrylic) acid (PAA) and poly(allylamine hydrochloride) (PAH). Cross-linking between PAA and …
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Evolution and Anatomy of Early Terrestrial Arthropods: Insights from the Rhynie Chert
… took their first steps. This thesis employs confocal laser scanning microscopy (CLSM) to investigate the anatomical and functional adaptations of Rhynie arthropods, addressing key questions about the evolution of arthropod terrestrialisation and the morphological innovations that enabled life …
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Three dimensional surface texture analysis for characterisation of enamel erosion
… enamel loss was measured using a white-light confocal profilometer and surface texture was measured using confocal laser scanning microscopy in combination with MountainsMap® surface analysis software. For the unpolished samples, only surface texture was measured. Statistical analyses were …
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Biofilm formation in clinical and laboratory strains of Escherichia Coli and Campylobacter Jejuni
… plate method, crystal violet staining and confocal laser scanning microscopy. The results for the E. coli laboratory isolatesi ndicated a decreasein biofilm fonnation in Luria-Bertani (LB) broth supplemented with lysed horse blood, whole and lysed human blood, human plasma, human serum and …
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Biomolecular effects and bioclinical applications of PARPs inhibitors
… and differentiation. By western analysis and confocal laser scanning microscopy (LSM), we analysed the effects of PJ-34 on PARP-1 expression, phospho-ERK and phospho-Elk-1 activation. The effect of MEK (mitogen-activated-protein-kinase-kinase) inhibitor PD98059 …
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Impact of Chloramination on the Development of Oligotrophic Biofilms
… weeks of biofilm growth till the end of week 10. Confocal laser scanning microscopy combined with quantitative analysis using COMSTAT program revealed that disinfection resulted in a reduction of average thickness and biomass volume by 83.6% and 81.8%, respectively, and an increase in compactness …
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Localized Excitation Fluorescence Imaging (LEFI)
… To address this deficiency, we developed a scanning fiber imaging method capable of nondestructive imaging of fluorescently labeled cells through a thick and optically opaque vascular scaffold, contained in a bioreactor. This imaging modality is based on local excitation of fluorescent …
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Development of Anti-corrosive Polyester Powder Coatings
… characterization techniques, including SEM/EDS, confocal laser scanning microscopy, Raman spectroscopy, XRD, and microCT, etc., were utilized for analyzing coating structures and identifying the passivation layer, corrosion products, and the underlying mechanisms.
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Foaming properties of whey protein isolate and [lambda]-carrageenan mixed systems
… while foam microstructures were visualized by confocal laser scanning microscopy (CLSM). For native WPI and [lambda]C mixed system, [lambda]C was needed in order for 5% WPI to form measurable foams. In all three WPI- [lambda]C systems at neutral pH, increasing [lambda]C concentration led to …
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Interactions between skimmed bovine milk and tea infusions under an acidulous environment: a thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy at Lincoln University
… using different microscopic techniques (SEM (scanning electron microscope) and CLSM (confocal laser scanning microscopy)). The secondary structure of proteins in ADGs were assessed by FT-IR, Circular dichroism and X-ray diffraction. Results showed that green, oolong and black tea infusions …
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