Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"conditioned media (CM)"”.
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Tracing of Alpha-to-Beta Cell Transition Following Treatment with CHIR-Injected Human Multipotent Stromal Cell Secreted Proteins
… bioengineered via WNT pathway activation (CHIR+ conditioned media; CM), secrete factors that promote beta-cell recovery in streptozotocin (STZ) treated NOD/SCID mice. Here, we investigated whether this regeneration process involves alpha-to-beta cell conversion, using a …
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Developing a model to assess the contribution of cytokeratin 19-expressing cells during multipotent stromal cell-induced islet regeneration
… multipotent stromal cells (hMSC) generated conditioned media (CM) that promoted β-cell regeneration in streptozotocin-treated mice. Ductal-derived endocrine progenitors, which have been shown to generate β-cells following pancreatic injury, represent a candidate for the ‘signal-receiving …
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MECHANISMS BY WHICH XRCC3 MEDIATES BREAST CANCER CELL INVASION VIA CYCLOPHILIN A
… that XRCC3 is upstream of these factors. Conditioned media (CM) from XRCC3-downregulated T4-2 cells enhances the invasion of naïve pre-invasive and invasive cells. Mass spectrometric analysis reveals that secreted cyclophilin A (CYPA) is upregulated in the CM by XRCC3-siRNAs. The addition …
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Analysis of drug resistance and the role of the stem cell niche in leukaemia
… investigation. Using microenvironment-mimicking conditioned media (CM), dexamethasone (a synthetic glucocorticoid) and etoposide to treat glucocorticoid-sensitive (C7-14) and glucocorticoid-resistant (C1-15) cells, pathways by which the microenvironment exerts its chemoprotective effect have been …
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Perivascular cell contribution to skeletal muscle repair following physiological injury
… 1 Hz) for 1 hr using a Flexercell Strain unit. Conditioned media (CM) was collected from strained (CM-S) or unstrained (CM-NS) pericytes 24 hr later and applied to C2C12 myoblasts for up to 72 hr. RESULTS: Pericyte transplantation following electrical stimulation inhibited inflammation based on …
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Presence and Role of Acanthamoeba in Wound Infections
… compared with heat-killed bacteria, bacteria conditioned medium (BCM) and Acanthamoeba conditioned media (CM). The exact trigger for the cell death in this study was not investigated but the relative contributions of apoptosis and necrosis were investigated using fluorescent technique, caspase …
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Molecular Study of Gβγ Dependent Breast Cancer Cell Migration and Invasion
… critical components of cancer metastasis. Conditioned media (CM) of NIH-3T3 fibroblasts contains chemoattractants that activate different GPCRs. Expression of βARKct attenuated NIH-3T3 CM-induced migration and invasion of both metastatic breast cancer MDA-MB-231 and MDAMB-436 cells by …
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Proteomic Characterization of Human Multipotent Stromal Cells Secreted Proteins with Therapeutic Potential for β-cell Regeneration
… proteins and refining current models of hMSC mediated β-cell regeneration. In working towards these goals, we first assessed the advantages and disadvantages of multiple fractionation techniques to help guide future experimental designs in general proteomic workflows. By applying these …
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Role of mural Cx43 in vascular interactions and its regulation by breast tumor cells
… mechanism(s) by which tumors prevent mural-cell mediated vessel stabilization have not been well elucidated. We demonstrate that heterotypic connexin 43 (Cx43)-dependent gap junction activity is required for mural cell-induced quiescence of endothelial cells, and that long term exposure of mural …
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Investigating the role of primary cilia loss in intrahepatic cholangiocarcinoma
… neutrophils and co-cultured these with organoid conditioned media (CM). In a Transwell migration assay, I showed that neutrophils exposed to non-ciliated CM migrated significantly less than when cultured in the presence of ciliated CM, suggesting that PC-loss on cancer cells directly impacts …
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Investigating interplay between human regulatory T cells and oligodendrocyte lineage cells
… consisted both of total CD4+ and CD8+ T cells. Conditioned media (CM) from these activated cultures were next harvested and added on human induced pluripotent stem cell (iPSC)-derived mixed neuroglial cells to examine changes in OPC differentiation and proliferation by immunocytochemistry. …
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Macrophage recruitment in DRG contributes to sensory axon regeneration after conditioning injury
… in the CI-induced axon regeneration. Macrophage conditioned media (CM) treated with cAMP did not exhibit neurite-growing activity. Contrarily, CM from neuron-macrophage co-cultures treated with cAMP significantly promoted neurite outgrowth, highlighting the requirement of neuron-macrophage …
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Characterization and application of adipose derived stem cells following burn injury
… treated with 100ng/mL of MCP-1 or 2% of ASC, conditioned media (ASC-CM) increased the expression of MCP-1, VEGF-165 and HIF-1α mRNA transcripts compared to the controls. Fibroblast migration was significantly increased in the presence of ASC-CM or with recombinant MCP-1 peptide (p<0.001). ASCs …