Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"conditional mutant"”.
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Multifunctional Regulators of Cardiac Disease and Development
… and MEF2D in chondrocytes using Mef2c and Mef2d conditional mutant alleles. From this study, I demonstrated that MEF2 proteins are also critical regulators of chondrocyte hypertrophy at least partly through their regulation of procollagen, type X, alpha 1 (Col10a1). I also explored another …
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Chromosomal instability and tumorigenesis : genetic analysis of the murine spindle checkpoint gene Mps1
… tumor formation, I generated mice containing a conditional mutant allele of the spindle checkpoint kinase MPS1 (MpslA) and induced its expression in thymocytes. I show that MpslA is a partial loss of function allele for spindle checkpoint activation which results in chromosome missegregation in …
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The germline- and tissue-specific effects of endogenous point-mutant p53
… mutations that result in accumulation of mutant p53 protein. These mutations may confer dominant-negative or gain-of- function properties to p53. To ascertain the physiological effects of tumor-associated point- mutations in p53, the structural mutant p53R172H and the contact mutant …
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Genetic and behavioral discrimination of dopamine 1 and 5 receptors in hippocampal dependent memory consolidation
… from D5Rs we have generated three strains of conditional mutant mice (D1 KO, D5 KO, D1/5 KO). I present data that shows distinct expression patterns within the hippocampus, the importance of D1 Rs and D5Rs in modulating hippocampal plasticity, and hippocampal dependent learning. These data …
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The role of Xist RNA in the maintenance of X chromosome inactivation
… somatic cells was investigated by generating a conditional allele of the Xist gene. A system was set up in which reactivation of two X-linked genes, the endogenous Hprt gene and an X-linked GFP transgene, can be quantitatively assessed. Mouse embryonic fibroblasts derived from mice carrying the …
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Development of Vessels, Airways and Cartilage Rings: The role of T-box genes
… potential of the allantois. Ex vivo, the Tbx4 mutant phenotype can be rescued using agonists of the Wnt signaling pathway and an inhibitor of the canonical Wnt signaling pathway phenocopies the Tbx4mutant phenotype in wildtype allantoises. In vivo, Tbx4 and Wnt2 double heterozygous placentas …
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Implicación de igf1r en inflamación pulmonar aguda y en alergia inducida por ácaros del polvo doméstico en ratones
… die shortly after birth, the generation of Igf1r conditional mutant mice would allow to avoid postnatal mortality. IGFs were reported to play a role in chronic lung pathologies including cancer, ARDS, COPD, pulmonary fibrosis and asthma, in which inflammation is a relevant component. Methods: …
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Neurogenesis and Gliogenesis from Ascl1 (Mash1) Expressing Progenitors in the Central Nervous System
… astrocytes and oligodendrocytes. Analysis of conditional mutants of Ascl1 demonstrated that Ascl1 is required for only one division of each lineage. Loss of Ascl1 results in a reduction of inhibitory dILA neurons and oligodendrocytes, but not excitatory dILB neurons and astrocytes. In chapter …
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Serum Response Factor Regulates Multiple Aspects of Central Nervous System Development
… of major axonal tracts in the forebrain. Conditional mutant mice lacking SRF in neural progenitor cells: Srf-Nestin-cKO) exhibit striking deficits in cortical axonal projections including corticostriatal, corticospinal, and corticothalamic tracts, and they show a variable loss of the …
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Functional analysis of tcf21 and tbx20 in zebrafish
… of these genes are essential for development and mutants do not survive. The solution to this problem is to engineer the gene so that it can be turned off at a specific time and in a specific cell type. A common method of this in the mouse model utilizes the Cre/loxP system: two loxP sites flank a …
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CATALYTIC-INDEPENDENT FUNCTIONS OF KDM6A IN B CELL MATURATION AND IMMUNE REGULATION
… recombination technology in vivo. In these conditional mutant mice, Kdm6a inactivation started in pro-B cells and reached completion in pre-B cells and later stages of B-cell lymphopoiesis. Kdm6a deficiency was dispensable for early B-cell differentiation but limited the re-entry of …
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Characterization of alternative NADH dehydrogenases in the respiratory chain of Toxoplasma gondii as a novel drug targets
… um auf diese Weise induzierbare knock-out Mutanten zu generieren. Depletion von TgNDH2-I in einer konditionalen knock-out Mutante hat weder Einfluß auf die Replikationsrate noch auf die in vitro Stadienkonversion der Parasiten. Daraus läßt sich der Schluß ziehen, dass TgNDH2-II allein für …
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Anti-Cancer Effects Of Garcinol In Pancreatic Cancer Transgenic Mouse Model
… to eight week old male KPC mice (kras and P53 conditional mutant) were divided into 4 groups( KC- cancer control; KGr- 0.05% Garcinol diet; KGm-Gemcitabine injected; KGG- Garcinol+Gemcitabine), littermates without mutation served as non- cancer controls were divided into 2 groups( CC- …