Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 88 for “"conditional knockout"”.
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Evaluation of Sex Differences in the Hippocampus and Pituitary of Egr1 conditional knockout mice mediated by Nestin-Cre
… gene and Nestin-Cre as a system for generating conditional knockout mouse strains. The Chapter begins with the identification of Egr1 gene and its protein structure, then proceeds to grasp its link to memory with behavior testing. The critical role of Egr1 in the pituitary and what cell …
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Defining the Role of Pannexin1 in Skeletal Muscle and Satellite Cell Function in vivo and ex vivo Using a Novel Conditional Knockout Mouse Model
… Using a novel skeletal-muscle-specific Panx1 knockout (Panx1^smKO) mouse model, this thesis highlights the influences Panx1 plays in muscular development and maintenance, as well as in muscle function. Loss of Panx1 led to a fiber-type switch coinciding with smaller fibers in both the tibialis …
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Genetische Inaktivierung des hyperpolarisations-aktivierten Ionenkanals HCN 4
The present work describes the development of knockout models for the genetic inactivation of the hyperpolarization-activated channel HCN4. The HCN4-subunit is expressed in the sinoatrial node of the heart, where it is probably involved in the generation of cardiac pacemaking. As a consequence, …
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Characterization of the TCOF1 Gene Using a Neuroblastoma Cell Line and a Mouse Model
… mice. In this study, we generated Tcofl conditional allele mice with loxP sites flanking exon 1. These mice were crossed with Wntl-Cre transgenic mice to generate a conditional knockout of Tcofl specifically in neural crest (NC) cells. Homozygous conditional knockout mice show …
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Glucose and Lipid Dysregulation Following Loss of Hepatic Aryl Hydrocarbon Receptor in Mice.
… metabolism. However, the generation of AhR knockout (AhR-KO) mice has shown physiological roles for the AhR in normal liver growth and development, insulin homeostasis, glucose homeostasis, and lipid metabolism. The liver is a major regulator of energy and glucose homeostasis and recent data …
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Analysis of Circadian Rhythm Using a Novel SCN-Specific Cre Transgenic Mouse Line
… SCN. Next, I generated NMS-Cre mediated Bmal1 conditional knockout animals to study behavioral rhythm output when most of the SCN neurons are without a molecular oscillator. The NMS-Cre(+);Bmal1flox/flox animals have essentially normal circadian rhythm of locomotor activity. Then, I generated …
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The role of ERα in the uterus during embryo implantation and in the ovary during ovarian tumorigenesis
… to placentation. Through the development of knockout mouse models, it is known that ERalpha, the predominant estrogen receptor in the uterus, is essential for the embryo to attach to these cells prior to invasion. However, we have uncovered for the first time the essential role of ERalpha in …
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The regulation of premature termination at divergent promoters
… of RNA Polymerase II. Here, we generated a conditional knockout of the essential RNA exosome subunit, Exosc3, in mouse embryonic stem cells. Removal of Exosc3 resulted in stabilization of polyadenylated uaRNAs, enhancer RNAs and long noncoding RNAs. In addition, promoter proximal pausing …
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The Role of Cleavage and Polyadenylation Spcific Factor 6 (CPSF6) in Hematopoiesis
… impacts on this process. To test this, we used conditional knockout mouse models to examine the function of <em>Cpsf6</em> in both embryonic and adult hematopoiesis. Our findings demonstrate that <em>Cpsf6</em> is essential for embryonic hematopoiesis but is dispensable for maintaining adult …
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Molecular targets of chromatin marks H3K4ME3, H3K9ME3 and H3K27ME3 in an adult germinal niche
… the role of EZH2 will be determined by EZH2 conditional knockout and overexpression models, using stereotaxic injections of novel Cre protein and lentiviral delivery of EZH2, respectively.
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Investigating The Role of The P63 Isoform Δnp63 In Lung Stem Cell Populations and Lung Cancer
… remains unclear. We utilized a ΔNp63-specific conditional knockout mouse model to determine ΔNp63’s role in lung progenitor cells and lung cancer. In vivo and in vitro experiments revealed a role for ΔNp63 in maintaining lung progenitor cells. ChIP-seq results indicate that deletion of ΔNp63 …
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The Impact of Acetylcholine Receptors on Osteocytes and Bone Mechanoadaptation
… Ca2+ signaling. We generated osteocyte-targeted conditional knockout mice to remove key components used by ACh receptors. We assessed tissue material make-up, strength, and formations rates to anabolic loading. We also used intravital imaging via two-photon fluorescent microscopy to visualize the …
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Genetic Analysis of The Hippo Pathway In Mouse Liver
… with different cre-mouse lines to generate conditional knockout mice. Results indicate a ubiquitous tumor suppression function of these components, predominantly in the liver. A further liver specific analysis of the deletion mutation of these components, as well as the <em>Yap/Taz</em> …
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Characterization of Cre Mouse Models to Target CNS Barriers for Generating Conditional Knockouts of ABC Transporters
… and have been studied using global transporter knockout mouse models. Since the CNS compartment and location of transporters is unique at each barrier site, a global knockout model does not address questions of how each barrier site contributes to ineffectiveness of drugs getting into the CNS. …
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The Effects of PTEN Deletion on Cell Size and Plasticity in the Hippocampus
… and synaptic structures and function in Pten-conditional knockout mice in which the gene was deleted from excitatory neurons during late development. Using 2-photon imaging, Golgi staining, immunohistochemistry, electron microscopy, and electrophysiologic tools, we determined that late Pten …
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Regulation of Toxoplasma Motility by Calcium-Dependent Protein Kinases
… gondii</italic> life cycle. Generating a conditional knockout, we showed that TgCDPK1 is required for microneme secretion during egress and invasion. The essential role of this kinase was further supported through the use of bulky pyrazolo [3,4-d] pyrimidine: PP) analogues that inhibit …
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The roles of Rb, p107, and E2f4 in bone formation and embryonic development
… lethality due to placental defects, we adopted a conditional knockout strategy to generate Rb-deficient embryos that survive to birth. This approach allows us to assess a role for Rb in skeletal development. We find that Rb-inactivation impairs the ossification of several bones. These bone defects …
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THE ROLE OF BMP SIGNALING AND ENDOGLIN IN REGULATION OF HEMATOPOIESIS
… in adult mice, using single and double Cre-based conditional knockout mice for the BMP signaling mediators Smad1 and Smad5. We demonstrate that BMP signaling deficient bone marrow (BM) competes normally with wild-type cells and display unperturbed self-renewal and differentiation capacity when …
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The Small GTPase Rheb Is Required for Spermatogenesis but not Oogenesis
… identified an essential role in spermatogenesis. Conditional knockout (cKO) of Rheb in the male germline resulted in severe oligoasthenoteratozoospermia and male sterility. More detailed phenotypic analyses uncovered an age-dependent meiotic progression defect combined with subsequent …
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Impaired Control of Chronic Herpesvirus Infection in Tissue Resident Macrophages During Coinfection with Intestinal Parasite and Other Cell-intrinsic Herpesvirus Interactions
… cell-specific MHV68 reactivation, we utilized conditional knockout mice to remove IL-4 receptor signaling on B cells or macrophages. We found that IL-4 signaling in combination with interferon-γ blockade reactivates MHV68 from latently infected macrophages, but not B cells. We continued our …
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