Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"complex III"”.
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Deconvoluting the Engineering and Assembly Instructions for Complex Iii Activity
In respiratory systems, membrane-bound Complex III catalyzes the oxidation of ubiquinone and the reduction of a soluble cytochrome with the bioenergetic formation of a transmembrane proton gradient (∆μH+). Complex III turnover is initiated by a unique two electron oxidation of ubiquinone at the Qo …
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CHEMICAL AND PHYSICAL CHARACTERIZATION OF COMPLEX III; THE OXIDATIVE REACTION MECHANISM
… 4-electron (CoO-depleted) and fully-reduced Complex III by ferricyanide. The nonlinear first-order plots of the oxidation of c(,1), were obtained for the reaction of 2-electron reduced sample at all tested ferricyanide concentrations. These data were explained by an extremely rapid …
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Effect of experimental complex III deficiency on respiratory chain assembly and function
The assembly of respiratory chain complexes in the mitochondrial inner membrane requires specific factors. Once assembled to form a mature functional complex, complex III (CIII) is a dimer consisting of two monomers, each with eleven subunits. To date, seven assembly factors for CIII are known, of …
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The Contextual Roles of Isocitrate Dehydrogenase-1 and Isocitrate Dehydrogenase-2 in Electron Transport Chain Complex III Deficiency
… subunit of electron transport chain complex III (ETCIII). Only a handful of UQCRC2-deficient patients have been described worldwide, and none have been subjected to a detailed metabolic analysis to understand the impact of defects in this protein. We used CRISPR-Cas9 gene editing to …
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The effect of hybridization on metabolism and hypoxia tolerance in sunfish
… and mitochondrial-encoded subunits of the enzyme complexes of oxidative phosphorylation. In my thesis, I examined metabolic properties and hypoxia tolerance of bluegill (Lepomis macrochirus), pumpkinseed (L. gibbosus), and their unidirectional F1 hybrids (male bluegill x female pumpkinseed). …
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Hibernation is super complex: dynamics of electron transport system supercomplexes
The electron transport system complexes form supercomplexes (SCs) within mitochondrial membranes, perhaps increasing respiratory capacity or reducing reactive oxygen species production. My project aimed to determine the abundance, composition, and stability of SCs in a hibernator. Hibernators have …
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Elucidating the mechanism of mitochondrial superoxide production in pro-inflammatory macrophages
… by reverse electron transport (RET) at complex I. However, this has not been demonstrated and the mechanism of mitochondrial superoxide production is unknown. Furthermore, the influence of LPS induced metabolic reprogramming on mitochondrial ROS (mtROS) production has not been …
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Computer modelling of metabolic adaptions during mitochondrial dysfunction and machine learning to predict novel mitochondrial disease genes
… set of rats administered a known mitochondrial complex III inhibitor. The analyses revealed many perturbed pathways which can be exploited as biomarkers of mild mitochondrial dysfunction, a condition which is currently clinically undetectable during the drug development process. To direct future …
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C-H activation: oxidative addition to an iridium(I) center and reactivity of the resulting iridium(III) species
Thermal reactions of the iridium complex, [Ir(COD)(PMe₃)₃]Cl (COD=1, 5 Cyclooctadiene), with benzene, benzaldehyde, pyridine, and furan yield the C-H addition products of mer-(Me₃P)₃Ir(Phenyl)(H)C! (1), mer-(Me₃P)₃Ir(COC₆H₅)(H)Cl (II), mer-(Me₃P)₃Ir(Pyridyl)(H)Cl (IID, and mer-(Me₃P)₃Ir(furyl)Cl …
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How do developers use parallel libraries?
… make their parallel code unnecessarily complex, (iii) applications of different size have different adoption trends. The library designers confirmed that our findings are useful and will influence the future development of the libraries.
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Characterization of alternative enzymes in bacterial respiration
… prokaryotes to the multicellular and highly complex eukaryotes and they largely derive this as a byproduct of respiration. Bacteria are known for their branched and highly modular aerobic and anaerobic respiratory chains. The branching refers to the presence of multiple respiratory enzymes …
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The Role of Mitochondrial Alternative Oxidase in Plant-pathogen Interactions
… O2 - burst prior to cell death. Also, we found iii that the appearance of HR and the appearance of the mitochondrial O2 - burst are delayed in transgenic plants lacking AOX. A similar delay is seen in transgenic plants treated with the complex III inhibitor antimycin A. In Wt plants, expression …
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Molekulare und biochemische Charakterisierung des mitochondrialen Translationsaktivators Cbs2p in Saccharomyces cerevisiae
Gegenstand der vorliegenden Arbeit ist das Kerngen CBS2 aus Saccharomyces cerevisiae. Cbs2p wird gemeinsam mit Cbs1p spezifisch für die Translation der Cytochrom b (COB)-mRNA in den Mitochondrien benötigt. Die Untersuchungen konzentrierten sich auf die Charakterisierung funktionell wichtiger …
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ROLE OF ENDOTHELIN-1 IN THE REGULATION OF THE SWELLING-ACTIVATED Cl- CURRENT IN ATRIAL MYOCYTES
… Blocking NOX with apocynin or mitochondrial complex I with rotenone both completely suppressed ET-1-induced ROS generation and activation of ICl,swell, indicating that ROS from both NOX and mitochondria were required to activate ICl,swell, and complete block by inhibitors of either ROS source …
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Molecular Mechanisms of Programmed Necrotic Death Initiated by Intrinsic Death Signals
… ROS production by upregulating the activity of complex III of the electron transport chain. My work concluded a key role for oxidative stress in the loss of long-term survival triggered by intrinsic death signals and revealed an important role for DeltaNp63alpha in regulating redox homeostasis. …
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INTRA-MITOCHONDRIAL INJURY DURING ISCHEMIA-REPERFUSION
… reperfusion. During ischemia oxidants from complex III oxidize cytochrome c, forming a peroxidase, which causes oxidative damage and depletion of cardiolipin. Depletion of cardiolipin disrupts normal physiology and augments cell death. Identification of the innovative pathobiology during …
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Mitochondrial metabolic suppression and reactive oxygen species production during hypometabolism in mammals
… largely from substrate oxidation inhibition at complex I and/or II. In skeletal muscle, state 3 respiration measured at 37°C was reduced up to 32% during hibernation. By contrast, in heart, state 3 respiration measured at 37°C was 2-fold higher during daily torpor in hamsters. Therefore, active, …
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Identification and functional characterisation of different inner mitochondrial membrane supercomplexes
… including oxidative phosphorylation (OXPHOS) complexes and transporters. Mitochondrial respiratory chain (MRC) complexes, which generate ATP by OXPHOS, associate in higher-order assemblies, known as supercomplexes (SC), that are structurally interdependent. Numerous patients carrying mutations …
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The role of Toll-like Receptor 4 in the Modulation in Skeletal Muscle Metabolism
… reactive oxygen species (ROS) from complex III. In conclusion, these data show that TLR4 activation elicits a change in mitochondrial substrate preference in that acetyl-CoA derived from pyruvate oxidation is the preferred substrate for the TCA cycle over that derived from …
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Structure-based theoretical characterisation of the redox-dependent titration behaviour of cytochrome bc1
… that represents complex III of the mitochondrial respiratory chain. It spans the inner mitochondrial membrane and uses the free energy of electron transfer from coenzyme Q (CoQ) to cytochrome c to shift protons across the membrane. The chemical energy of reduced …
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