Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 24 for “"complementation assay"”.
-
Development of high-throughput screening assays to identify small molecule inhibitors of Plasmodium falciparum Hsp70-1
… the shortage of simple, cost-effective screening assays. Heat shock proteins (Hsps) are a special class of molecular chaperones essential for parasite survival and development within the human host. Hsps are also implicated in drug resistance. Hsp70 forms an integral part of the molecular …
-
Investigation of the role of the GGMP motif of Plasmodium falciparum Hsp70-1 on the chaperone function of the protein and its interaction with a co-chaperone, PfHop
… of PfHsp70-1, DnaK and the GGMP variants, a complementation assay in E. coli dnak756 cells whose Hsp70 is functionally compromised was conducted. The PfHsp70-1 GGMP variants were able to suppress the thermosensitivity of the E. coli cells. However, the Investigation of the role of GGMP motif …
-
Characterization of Ligand-Induced Conformational Changes in the EGF Receptor
… cells in real time using luciferase fragment complementation imaging. We find that these conformational changes are unique to the full-length activated EGF receptor. These studies identified a novel conformational change that was dependent on MAP kinase activation and desensitization of the …
-
EXAMINING THE ROLE OF THE XAB2 PROTEIN IN HOMOLOGOUS RECOMBINATION
… a complex during HR. By using an end resection assay and cell biology analysis, I found that XAB2 is essential for 5’ – 3’ end resection, an intermediate step common to both HDR and SSA pathways. With a functional complementation assay I developed, I have shown that specific TPR regions are …
-
Innate Immune Sensing and Signaling of Cytosolic DNA
… of STING remains elusive. Using a cell-free complementation assay, we identified cyclic GMP-AMP (cGAMP), as a novel eukaryotic second messenger generated by DNA stimulated or DNA virus infected cells. cGAMP contains a unique phosphodiester linkage combination (both 2'-5' and 3'-5'), for which …
-
A single molecule approach to investigate how AP1 transcriptional regulators find their target sites on DNA
… will be investigated. The Protein Fragment Complementation Assay (PCA) derived inhibitor FosW is also capable of interfering with its target cJun. To study these proteins, DNA tightropes were created where single strands of λ, pUC19, pUCap1 and target-free λ (TFλ) DNA were suspended above …
-
Intracellular peptide library screening to derive inhibitors of Parkinson's disease associated α-synuclein aggregation
… a multiplexed intracellular Protein-fragment Complementation Assay (PCA) library screening system. Successfully selected peptides must bind to full length α-syn and lower its toxicity to confer bacterial growth. PCA selected peptides were characterized using several biophysical assays and a …
-
New reporters of protein trafficking and protein-protein interactions in live cells
… (PPIs) in live cells by means of a ribozyme complementation assay, but we found that the strategy was limited by the interaction affinity constraints and by low ribozyme activity in cells. We then sought to still detect interactions among endogenous proteins but in fixed cells. We devised an …
-
Atp-Citrate Lyase Links Cyclin E to Cellular Metabolism In Breast Cancer
… hypothesis, we used a retrovirus-based protein complementation assay (RePCA) to identify LMW-E (T1) protein-protein interactions in breast cancer. Using this methodology, we found ATP-citrate lyase (ACLY) as a novel interacting protein of LMW-E (T1) in the cytoplasm. ACLY is a 125kDa …
-
Engineered heme peroxidases as genetically encoded probes with diverse applications in cell biology
… reporter enzyme widely used in bioanalytical assays, has been utilized for EM because of its ability to generate electron-dense deposits. In addition, HRP produces extremely sensitive chemiluminescent, colorimetric, and fluorescent readouts and has applications for promiscuous protein labeling …
-
Exploring candidate genes and rhizosphere microbiome in relation to iron cycling in Andean potatoes
… genes orthologous to Arabidopsis ferrome, yeast complementation assay and genetic transformation to avoid the limitation from a single approach. We revealed several candidate genes potentially associated with potato plant Fe acquisition, PGSC0003DMG400024976 (metal transporter), …
-
Manipulation of the Moloney Murine Leukemia Virus Envelope Protein in an Effort to Develop Directly and Indirectly Targeted Retroviral Vectors for Use in Human Gene Therapy
… breast cancer cell line, and (3) a eukaryotic α-complementation assay is not sensitive enough to detect cell-cell fusion at levels below that of the wild type MoMLV envelope with its natural receptor. While much investigation remains to be done, these observations will help pave the way to the …
-
Influenza Viral Protein Interaction Detecting Methods Development and A.M1-host Interplay Network Investigation
… subunits interaction. In addition to two protein complementation assays, Split luciferse complementation assay (SLCA), and Bimolecular Fluorescence Complementation (BiFC), LUMIER (luminescence-based mammalian interactome mapping) assay were also developed and used in this study. We demonstrated …
-
Analysis Of The Structure And Function Of A Timp-1/cd63 Complex And Its Relationship To An Mt1-Mmp/cd63 Complex
… interaction analysis using protein complementation assay (PCA) of the interaction of TIMP-1 and CD63. CD63 is a tetraspanin, whose major functional properties are not well understood. We have previously identified CD63 as a non-MMP protein and a transmembrane (TM) protein with which …
-
In cellulo and biophysical exploration of the role of GGMP residues of Hsp70
Hsp70 is a prominent molecular chaperone. Structurally, Hsp70 is composed of two domains, C-terminus substrate binding domain (SBD) and nucleotide binding domain (NBD). The NBD of Hsp70 is responsible for its ATPase activity. Some Hsp70s of parasites particularly those of apicomplexa are marked by …
-
Development of Bioluminescent Biosensors Monitoring Activity of the Merlin Tumour Suppressor
All solid tumours share the ability to circumvent or overcome contact inhibition of proliferation (CIP), the process by which cell-cell contacts engage signalling to stop proliferation. Merlin, an ERM-like protein, plays a crucial role in CIP and is inactivated in a wide variety of cancers, …
-
Induced Degradation of G-Protein-Coupled Receptors Through Proteolysis Targeting Chimeras
… degradation. We used both a split-luminescence complementation assay and immunoblot assay to see the effects of the HaloPROTAC3 on the protein abundance. Additionally, we used a secondary messenger d-myo-inositol-1-phosphate (IP1) accumulation assay to measure the changes in activation of the …
-
Restored iron transport by a small molecule promotes absorption and hemoglobinization: discovery, development, and mechanistic studies
… molecule candidate. In a modified functional complementation assay, the small molecule natural product, hinokitiol, restored growth to yeast missing the iron transporting complex Fet3Ftr1. Hinokitiol promoted iron uptake, and concomitantly restored cell growth back to wild-type levels. In …
-
FLP-mediated conditional loss of an essential gene to facilitate complementation assays
Commonly, when it is desirable to replace an essential gene with an allelic series of mutated genes, or genes with altered expression patterns, the complementing constructs are introduced into heterozygous plants, followed by the selection of homozygous null segregants. To overcome this laborious …
Page 1 of 2