Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"compensatory mutations"”.
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Investigating the Double-Edged Sword of Rifampicin Resistance in Mycobacterium Tuberculosis
… taken for six to nine months. RifR is caused by mutations in rpoB, the gene encoding the β subunit of RNA polymerase (RNAP), the target of Rif. These mutations block Rif binding but also alter the shape of the RNA exit channel, potentially disrupting transcription elongation. As is true for many …
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Investigating the Role of Stem-Loop 1 in the Assembly Process of HIV-1
… accelerated by introducing previously identified compensatory mutations into the SP1 and NC domains of Gag, in a manner reminiscent of the actions of maturation inhibitor resistance mutations. The effects of the compensatory mutations were also dependent on intact late domain motifs. These data …
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Studies into Transcriptional Pausing Regulators and Drug Resistance in Mycobacterium Tuberculosis
… a fitness defect. However, there are secondary, compensatory mutations existing in the RNAP and other transcription factors that relieve hyper-termination effect and restore fitness to the cell. Using biochemical and functional, in vivo studies, we showed the first mechanism of RifR compensation …
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Understanding the evolutionary radiation of the megadiverse monkey beetle fauna (Scarabaeidae: Hopliini) of South Africa
… T lymphocyte (CTL) responses. Evolved evasion mutations that undermine host immune responses pose a major challenge to the development of such vaccines. Understanding the mechanisms that selectively favour the emergence of CTL evasion mutations in vivo and the impact of these mutations on both …
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Impact of Cytotoxic T Lymphocyte (CTL) escape mutations in acute/Early HIV-1 Subtype C Infection on Disease Progression
… T lymphocyte (CTL) responses. Evolved evasion mutations that undermine host immune responses pose a major challenge to the development of such vaccines. Understanding the mechanisms that selectively favour the emergence of CTL evasion mutations in vivo and the impact of these mutations on both …
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Model studies aimed toward constructing PI3K pathways in leukemia T-cells from shRNA knockdowns
… less vulnerable to deleterious side effects or compensatory mutations. However, despite their potential, methods to reconstruct networks are often specific to experimental technique and their exact ability to accurately capture networks is unexplored. Motivated by a shRNA knockdown experiment on …
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Activities of Bacteroides Conjugative Transposons: CTnGERM1 and CTnDOT
… of ribosomes to the ribosome binding site. Mutations that abolished base pairing in the stem of this putative hairpin structure made GUS production as high in the absence of Tc as in Tc-stimulated cells. Compensatory mutations that restored the hairpin structure led to a return of regulated …
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The genetic landscape of protein-protein interaction specificity
… of fitness effects for individual interface mutations measured across hundreds of genetic backgrounds. We show that positive and negative contributions to specificity are neither inherently coupled nor mutually exclusive. Further, we argue that the wild-type antitoxin may be optimized for …
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Genetic and Biochemical Analyses of the Flaviviridae Capsid Proteins
… defective HCV core mutants could be rescued by compensatory mutations in p7, NS2, and NS3, adding to accumulating evidence that these nonstructural proteins are important for virion morphogenesis. The functional determinants of an analogous BVDV protein, uncleaved NS2-3, in infectious virus …
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Evolution and Regulation of Virulence and Antimicrobial Resistance in Pseudomonas aeruginosa
… by which avirulent strains become virulent via compensatory mutations. I constructed and characterised an extensive collection of mutants in a mutant that is defective in the SR (due to deletion of the relA and spoT genes) and discovered that QS-dependent virulence factor production can be …
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Regulation of Human Telomerase Alternative Splicing
… RNA:RNA pairing may regulate splicing of hTERT. Mutations within the repeat sequence that abolish exon skipping were corrected by compensatory mutations in the pre-mRNA. To identify trans-acting regulators of hTERT alternative splicing, the minigene was modified into a dual-luciferase reporter …
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Evolutionary influences on the reduction in enzootic circulation and human incidence of western equine encephalitis
… levels. My studies identified six nonsynonymous mutations that were phylogenetically significant for the evolution of WEEV through the 20th century. I also found that these mutations have a phenotypic effect on WEEV’s enzootic hosts. Using competitive fitness assays, contemporary mutations have a …
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Auto-tethering as a selection mechanism for recognition of multimeric substrates by the AAA+ unfoldase ClpX
… binding sites in the transpososome containing compensatory mutations. This work can shed light on the division of labor required to mediate auto-tethering in the transpososome as well as in other multimeric substrates of CIpX.
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Functional Characterization of the Mammalian TRPV4 Channel: Yeast Screen Reveals Gain-of-Function Mutations
… and mechanosensitive processes; recently, mutations of TRPV4 have been linked to human skeletal and neurodegenerative disorders. We obtained a loss-of-function variant of TRPV4 containing the substitutions K70E (N-terminal tail) and M605T (intracellular linker between transmembrane helices …
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Impatto di rifaximina sul microbiota intestinale: selezione di bifidobatteri antibiotico resistenti
… selection of resistant mutants. Since point mutations in rpoB have been indicated as representing the principal factor determining rifampicin resistance in E. coli and M. tuberculosis, whether a similar mechanism also occurs in Bifidobacterium was verified. The analysis of a 129 bp rpoB core …