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Showing 1 to 17 of 17 for “"compatible solutes"”.
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Molecular characterisation of the mechanisms of compatible solute accumulation in Listeria monocytogenes
… of a restricted range of low molecular mass solutes compatible with cellular function. Accumulated to high internal concentrations in hyper-saline environments, compatible solutes, either transported into the cell or synthesized de novo, play a dual role: helping to stabilize protein …
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Development of SNAP-tag-based fusion proteins targeting HIV-1 viral reservoirs
… by osmotic stress expression in the presence of compatible solutes in the periplasmic space of E. coli. SNAP-tag is a self-labelling protein that covalently binds benzylguanine (BG)-modified substrates in a 1:1 stoichiometric ratio. When recombinantly fused to any protein of interest, SNAP-tag …
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Physiological Responses of Cotton Genotypes to Water-Deficit Stress during Reproductive Development
… in osmotic adjustment through accumulation of compatible solutes, increased activity of enzymes, and perturbation of carbohydrates metabolism in leaves and flowers of cotton plants, and differences in drought tolerance among the genotypes would exist. Therefore, field and growth room …
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USE OF MOLECULAR APPROACHES TO STUDY THE OCCURRENCE OF EXTREMOPHILES AND EXTREMODURES IN NON-EXTREME ENVIRONMENTS
… resonance spectroscopy (NMR) to detect the compatible solutes and atomic emission spectophotometery (AES) to measure the cellular potassium ions. In addition, ultraviolet-type C tolerant bacteria were isolated from terrestrial environments using phylogenetic analysis of the 16S rRNA gene …
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Microbial Evolution In Sea Ice: Communities To Genes
… proteins involved in the degradation of abundant compatible solutes like glycine betaine. Furthermore, the positive growth of C. psychrerythraea strain 34H on sarcosine (a derivative of glycine betaine) as a sole carbon and nitrogen source suggested that the laterally transferred genes were …
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An integrated geochemical and microbiological investigation of sulphate reduction in hypersaline pans
… and 134-244 at highly saline pans. The use of compatible solutes, not K+, for osmoprotection, was inferred. Half-saturation constants measured here ( 64-780mM) are the first reported for hypersaline sites and were much higher than previously measured in other environments, implying greater …
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Metabolite profiling of Eragrostis nindensis during desiccation and recovery
… reactive oxygen species quenchers and compatible solutes that replace water during desiccation. The identified metabolites and metabolic process provide a great insight into the goal of improving drought tolerance in orphan and drought-sensitive crops.
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Implications of Microcystin Production in Microcystis aeruginosa PCC 7806
… upon high light exposition. Accumulation of compatible solutes in the ΔmcyB mutant suggests a role of microcystin in fine-tuning the metabolic flow to prevent stress related to excess light, high oxygen concentration and carbon limitation. Co-analysis of the widely used model cyanobacterium …
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Transcriptomic and proteomic analysis of anhydrobiosis in Panagrolaimus superbus and Caenorhabditis elegans dauer larvae
… molecular shield activity, antioxidants, compatible solutes, the ubiquitin proteasome system, autophagy, DNA repair, cellular repair and response to pathogens. While there was much overlap in the identities of the genes and proteins which were up-regulated in response to dehydration, …
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Analysis of the nuclear proteome of the resurrection plant Xerophyta viscosa (Baker) and its response to dehydration stress
… such as antioxidants, molecular chaperones and compatible solutes (33%). This reflects the importance of strong defense systems in preventing lipid peroxidation, protein aggregation, membrane leakage and maintaining the integrity of cellular structures during dehydration and in the dried state. …
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Molecular studies on plants to enhance their stress tolerance
… toleranc via a more pronounced accumulation of compatible solutes under stress.
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The osmotic stress response of ale and lager yeast
… osmotolerance is achieved when cells accumulate compatible solutes in order to increase intracellular osmolarity thus promoting retention of cellular water and as a result maintenance of turgor and viability. Yeast cells preferentially accumulate the polyhydric alcohol glycerol, although a …
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Next-Generation version of pseudomonas exotoxin a based immunotoxins with reduced immunogenicity for triple negative breast cancer (TNBC) treatment
… osmotic stress conditions in the presence of compatible solutes. The 72 kDa, His10-tagged fusion proteins were purified using a two- step Immobilised Metal-ion Affinity Chromatography (IMAC). Specific binding of these recombinant immunotoxins to LIV-1- and TROP2-positive breast cancer cell …
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A metabolomics-based approach to study abiotic stress in <em>Lolium perenne</em>
… genotype Cashel and an increase in sugars and compatible solutes in the drought-tolerant PI 462336 genotype. Sugars exhibiting a significant increase included, raffinose, trehalose, glucose, fructose and maltose. Raffinose was identified as the metabolite exhibiting the largest accumulation …
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Elucidating the Molecular Pathways Involved in the Fitness and Water Stress Response of Pseudomonas synxantha 2-79 in the Plant Rhizosphere
<p><em>Pseudomonas synxantha</em> 2-79 is a biocontrol agent that represents beneficial indigenous rhizobacteria that are broadly distributed in the Pacific Northwest, USA and flourish in the rhizosphere of commercially grown wheat under surprisingly arid conditions. The molecular adaptation of …
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Development of recombinant immunotoxins targeting overexpressed cell surface biomarkers trophoblast cell-surface antigen 2 and mesothelin for cervical cancer treatment
… under osmotic stress in the presence of compatible solutes in Escherichia coli (BL21 DE3) cells. The rITs were extracted using sonication in a lysis buffer with protease inhibitors to recover soluble active proteins. Once expression was confirmed, purification was performed using two-step …
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Development Of Recombinant Immunotherapeutics for Triple-Negative Breast Cancer (TNBC)
… osmotic stress conditions in the presence of compatible solutes. The rITs were then purified by immobilized metal ion chromatography (IMAC) and size exclusion chromatography (SEC). Confirmation of full-length rITs was done by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS PAGE) …