Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"commercial kit"”.
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Anti-platelet aggregation activity of melaleuca bracteata var.revolution gold derived betulinic acid and its derivatives
… as platelets aggregate, was investigated using a commercial kit and Fura 2/AM respectively. The anti-acetylcholinesterase activity of the triterpenes was also investigated using a commercial kit. The compounds were fed to rats and the tail bleeding time was used to determine the ex vivo …
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Comparison of Commercial DNA Extraction Kits with That of Organic Extraction Procedures Using Simulated Forensic Evidence Samples
… using the ldentifiler human identification kit (Applied Biosystems, Foster City, CA) and analyzed using the ABI 310 genetic analyzer to assess the quality and purity of the recovered DNA from the samples. For the straight extraction both methods recovered DNA, but the commercial kit …
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Molecular diagnostics, genetic diversity and generating infectious clones for cassava brown streak viruses
… infected cassava plants and compared with the commercial kit RNeasy (Qiagen) for sensitivity and reproducibility. Results showed that both protocols were reliable but CTAB was more cost-effective and ideal for resource-poor laboratories. Mixed infections of cassava mosaic begomoviruses (CMBs) …
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Effect of autologous macrophage therapy in cirrhosis in response to individual immune reparative pathways: developing a novel therapy
… and chemokine pre and post treatment using a commercial kit. Moreover we developed a protocol for P13- magnetic resonance spectrometry (MRS) for the analysis of the metabolically active liver parenchyma. Data from the phase 1 trial were used to improve the autologous cellular produce and phase …
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The utility of a real-time PCR to detect Leptospira in a routine diagnostic setting
… chain reaction assays and a locally available commercial kit. Although our assay needs further optimization, we demonstrated that the PCR reliably detected 100 copies and 1000 copies of Leptospira rrs and lipL32 targets respectively. To test specificity, we did real-time PCR with pure DNA from …
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Detection of the calreticulin type 1 and type 2 mutations in a group of myeloproliferative neoplasm patients using molecular methods
… to detect these mutations. A conventional PCR commercial kit was also compared to that of the developed conventional PCR. For this study 24 blood samples were collected from the tertiary hospital haematology clinic, 24 archived DNA samples from a colleague's previous JAK2 mutation study were …
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Expression of angiogenic growth factors in laryngeal carcinoma
… Protein was extracted from the tissue using a commercial kit (Proteojet lysis reagent, Fermentas Life Sciences, York, UK, Calbiochem/Merck, Nottingham, UK) and the expression of 55 angiogenesis-related proteins was determined using a human angiogenesis array (R&D systems). Subsequently the …
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Phylogenetic and physiological characterization of deep-biosphere microorganisms in El’gygytgyn Crater Lake sediments
… DNA pools with a total DNA pool extracted with a commercial kit, significant differences in the microbial composition of all three pools (mean distance of relative abundance: 24.1%, mean distance of OTUs: 84.0%) was discovered. In particular, the total DNA pool covers significantly fewer taxa than …
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Využití různých metod izolace DNA baktérií mléčného kvašení v molekulárně biologických metodách
… magnetickými částicemi a izolace komerčním kitem. DNA byla izolovaná ze tří doplňků stravy a to Biopron 9 premium, Linex forte a GS Laktobacily forte 21. Čistota a koncentrace izolované DNA byla zjištěna spektrofotometricky. Přítomnost jednotlivých kmenů DNA v potravinových doplňcích byla …
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Pharmacogenomics of sickle cell disease therapeutics: pain and drug metabolism associated gene variants and hydroxyurea-induced post-transcriptional expression of miRNAs
… blood, following instructions on the available commercial kit [QIAamp DNA Blood Maxi Kit ® (Qiagen, United States)]. Genotyping (TaqMan and MassArray) was performed for 40 variants in 17 pain-related genes, three fetal haemoglobin (HbF)-promoting loci, two kidney dysfunction-related genes, and …