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Showing 1 to 1 of 1 for “"combined annealing/extension protocol"”.

  1. Optimization of BRAF V600 Assay as A 2-Step Real-time PCR Protocol

    … prostate cancer. Currently, the established qPCR protocol for the QClamp® BRAF Mutation Detection Assay is comprised of a 4-step procedure: <em>Denaturation, XNA Annealing, Primer Annealing and Extension</em>. The purpose of this experiment was to test the feasibility of optimizing this assay to a …

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