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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 43 for “"colorimetric assay"”.
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A Possible Role of Ascorbate in Boron Deficient Radish (Raphanus sativa L. cv. Cherry Belle)
… in four different strengths of boron solution. A colorimetric assay for phosphorus was performed both before and after supplementation. Results, however, were inconclusive.
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NAD(P)H:QUINONE OXIDOREDUCTASE (NQO1)-DIRECTED LAVENDAMYCIN ANTITUMOR AGENTS: STRUCTURE-BASED DESIGN, MOLECULAR MODELING AND STRUCTURE-ACTIVITY STUDIES
… NQO1 were studied using a spectrophotometric assay. These structure-activity relationship (SAR) studies determined key structural features that were required for lavendamycin substrate specificity. Cytotoxicity toward human colon adenocarcinoma NQO1-deficient (BE) and NQO1-rich (BE-NQ) cells …
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NAD(P)H:QUINONE OXIDOREDUCTASE (NQO1)-DIRECTED LAVENDAMYCIN ANTITUMOR AGENTS: STRUCTURE-BASED DESIGN, MOLECULAR MODELING AND STRUCTURE-ACTIVITY STUDIES
… NQO1 were studied using a spectrophotometric assay. These structure-activity relationship (SAR) studies determined key structural features that were required for lavendamycin substrate specificity. Cytotoxicity toward human colon adenocarcinoma NQO1-deficient (BE) and NQO1-rich (BE-NQ) cells …
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Cloning and characterization of the Pichia Pastoris PMR1 gene
… using 3,3',5,5' tetramethylbenzidine (TMB) colorimetric assay and western analysis. β-gal expression and secretion was determined by western analysis. Secretion in P. pastorius Δpmr1 for both heterologous proteins showed no appreciable difference compared to wild type, nor did P. pastoris …
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Development of a Field-Use Paper-Based PKU Test Using Colorimetric Readout
… PKU test, based on a well-known enzyme colorimetric assay (ECA), which is applicable to low-resource settings. Phenylalanine dehydrogenase (PheDH) catalyzes the reaction between phenylalane (Phe) and NAD+ to produce phenylpyruvate, NH4, and NADH. Subsequently, NADH reacts with nitroblue …
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Observing Renal Responses to Endurance Cycling in the Heat
… and post-event. Serum creatinine was measured by colorimetric assay and analyzed via the Jaffe reaction, and creatinine clearance was estimated. An ELISA kit was used to measure serum neutrophil gelatinase-associated lipocalin. RESULTS: A net fluid loss of 1.3 ± 1.1 kg was observed. Urine specific …
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DEVELOPMENT OF A RAPID DIAGNOSTIC ASSAY FOR THE COLORIMETRIC DETECTION OF BACTERIAL AND VIRAL PATHOGENS USING LOOP-MEDIATED ISOTHERMAL AMPLIFICATION COUPLED WITH A PEPTIDE NUCLEIC ACID AND GOLD NANOPARTICLE REPORTER SYSTEM
… (AuNP) detection system that results in a colorimetric assay. By employing modified LAMP primers, the target-dependent, co-amplification of a novel, sequence is generated, which serves as the target for PNA/AuNP sequence-specific detection. This investigation verified the generation of a …
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Biochemical Evaluation of SRH Analogs as Potential LuxS Inhibitors
… goal of this project is to establish biochemical assays for the evaluation of small molecules as potential LuxS inhibitors. The first assay is a conventional colorimetric assay that utilizes Ellman’s reagent to quantify the homocysteine byproduct of DPD production by LuxS. For this assay purified …
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Fructose and the Maillard reaction
… diseases is unknown, largely because assays for glycation underestimate, or do not detect, fructation (Ahmed N& Furth A. J. 1992, Clin. Chem. X8,1301-1303). In vitro radiolabelling illustrates that incorporation of fructose into protein, through the Maillard reaction, is more swift …
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Radiobiological evaluation of new boron delivery agents for boron neutron capture therapy
… (BPA). In vitro evaluations utilized a colorimetric assay and 96-well plates to minimize the quantities of compound required for testing. The assay was optimized for the murine SCCVII, squamous cell carcinoma to determine the chemical toxicity and relative cellular uptake of a compound. …
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The principal protease system in bovine milk
… shown to occur in human milk. We used a coupled colorimetric assay to demonstrate the occurrence of a fibrin-independent plasminogen activator (similar to u-PA) in bovine skimmilk. The occurrence of a u-PA like activator in bovine milk was further confirmed in co-polymerized gel electrophoresis. …
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Leukocyte Response to Elastin-Like Polypeptide Coatings
… coverage, respectively, as determined by the colorimetric assay, FastinTM Elastin. These surfaces were exposed to flowing citrated whole blood for surface and bulk evaluation of leukocyte activity using scanning electron microscopy and flow cytometry, respectively. Little leukocyte activation …
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Modified alginates as a matrix for gene transfection in a HeLa cell model
… protein, P-Galactosidase, was quantified using a colorimetric assay. Compared to the controls, the modified compounds showed moderate to significant improvements in transfection capability, with one exception. The matrix prepared by modifying alginate with diaminoacridine hydrochloride proved …
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Comparative efficacy of azole compounds targeting sterol 14-demethylase against multiple isolates of Acanthamoeba castellanii and polyphaga
… and Acanthamoeba polyphaga using a modified colorimetric assay. Sequence analysis was also carried out in order to prove the existence of the putative target enzyme of the azoles. The results indicate that azoles have an inhibitory effect on Acanthamoeba spp. growth although the effectiveness …
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<i>PTHR1/SOX9</i> and <i>IDH1/IDH2</i> Relative Expression in Primary Chondrocyte and Chondrosarcoma Cells Under the Synergistic Influence of Inducible Hypoxia and Extracellular Acidosis
… D-2-Hydroxygluterate (D-2-HG). Our colorimetric assay data suggest that D-2-HG concentration levels are 10.5-fold and 6-fold more elevated in JJ012/SW1353 respectively than in the <em>IDH</em> wild type CON5. Our gene expression data indicates that both inducible hypoxia and …
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Exploring the interaction between asymmetric phosphonates and acetylcholinesterase. Probing the gorge and P-site via customized covalent modification
… electric eel AChE (EEAChE) were determined via colorimetric assay and the dansyl containing FPs were demonstrated to be the most potent. In addition, the binding effects of the chromophore and FP moiety to protein were evaluated and results demonstrated that the size and structure of chromophore …
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Exploring the interaction between asymmetric phosphonates and acetylcholinesterase. Probing the gorge and P-site via customized covalent modification
… electric eel AChE (EEAChE) were determined via colorimetric assay and the dansyl containing FPs were demonstrated to be the most potent. In addition, the binding effects of the chromophore and FP moiety to protein were evaluated and results demonstrated that the size and structure of chromophore …
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Developing Low-GN and GN-zero Winter Malt Barley for the Eastern United States
… VA and screened for GN content using a colorimetric assay. All VT lines tested positive for presence, and 93 were sampled for micromalting and GN quantification 2023. These lines, along with controls, were genotyped using genotyping-by-sequencing (GBS) and evaluated for genomic …
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Structural Elucidation of [RNQ+] prions Transmissible Infectivity
… propagation, we have taken advantage of the colorimetric assay of the yeast prions [RNQ+] and [PSI+]. These prions result from the aggregation of the proteins Rnq1 and Sup35, respectively. Changes in phenotype allows for the investigation of the structure and toxicity of yeast fibrils formed …
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