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Showing 1 to 20 of 25 for “"colony-forming-unit"”.
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Experiment-Based Quantitative Modeling for the Antibacterial Activity of Silver Nanoparticles
… analysis based on kinetic growth assay and colony forming unit assay has been carried out to study the antimicrobial effect of Ag nanoparticles against Escherichia coli (E. coli) bacteria. It was observed that the presence of Ag nanoparticles increased the lag time of bacterial growth while …
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Molecular substrate design for the selective adhesion, proliferation and differentiation of marrow connective tissue progenitors
… CFU formation of marrow-derived CTPs using colony forming unit (CFU) assay.
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Cellular Responses to Short-Term Mechanical Loading: Relative Involvement of Osteoblasts, Pre-Osteoblasts, and Osteoprogenitor Cells
… stages of the osteoblast lineage (osteogenic colony forming unit (CFU-O), pre-osteoblast, and osteoblast) to short-term, external mechanical loads applied to the tibiae of rats. The results showed that loading protocols sufficient to induce lamellar bone formation do not affect endocortical …
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Surface Engineered Novel Patterned Polymers to Remove Pathogenic Biofilms from Human Skin. Effective Removal of Antimicrobial Resistant Bacteria from Chronic Wounds
… S. aureus and P. aeruginosa, as determined using Colony Forming Unit (CFU) analysis (*p<0.01), Scanning Electron Microscopy (SEM) (*p<0.01) and Confocal Scanning Laser Microscopy (CSLM) (*p<0.01). The released triclosan was not cytotoxic against either bacteria or primary cultures of human dermal …
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Haematopoietic progenitor cells in carotid disease
… by flow cytometry (CD133+ve/CD34+ve) and early colony forming unit assay (eCFU). HPC function was measured by migration assay and by staining for senescence associated B-galactosidase (SA-Bgal). HPC number and function was then measured in 62 patients undergoing CEA pre-operatively, 1 day post …
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Investigating the potential of Sodium Butyrate to Control Salmonella Enteritidis in Poultry
… sodium butyrate reduced SE adhesion by ∼1.7 Log colony forming unit (CFU)/mL and ∼1.8 Log CFU/mL and invasion by ∼2 Log CFU/mL and ∼2.93 Log CFU/mL respectively in primary chicken enterocytes (P</p>
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Fabrication of graphene-based resistive sensor for eschericia coli bacteria sensing
… the number of the bacteria from 4 to 273 colony forming-unit (cfu) result decreasing the resistance from 4.371 to 3.903 ohm gradually. Finally, the sensor was successfully designed using SolidWorks and fabricated by integrating graphene film as a sensing layer with Arduino …
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Sparse Sample Detection Using Magnetic Bead Manipulation on a Digital Microfluidic Device
… sparse sample detection system is as low as 1 Colony Forming Unit/mL (CFU/mL).</p>
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DEHYDRATION AND MICROBIAL IMPACTS ON WATER UPTAKE AND POSTHARVEST QUALITY OF CUT LILY
… water. Bacterial concentrations of 107 cfu·mL-1 (colony forming unit per mL) caused inferior postharvest quality. The abundance of bacteria was greatest in the lower stem, especially in the lowest 15 cm. By using 16S rRNA gene sequencing, we found different flowers (rose, lily, and gerbera) affect …
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Role of epigenetics in hematopoietic stem cell development
… blood cancers and over one million people in the United States living with one of these disorders. Bone marrow transplants have good outcomes, but these procedures require a donor who is a perfect match, and thus many patients are unable to receive treatment. It is important to find …
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A Bifunctional Nanocomposites Based Electrochemical Biosensor for In-field Detection of Pathogenic Bacteria in Food
… S. Typhimurium within the range of 102 to 106 colony forming unit (cfu)/ml in the pure culture. The limits of detection (LODs) of E. coli O157:H7 in ground beef and S. Typhimurium in chicken carcass rinse water were 2.05 x 103 cfu/g and 1.04 x 103 cfu/ml, respectively. The second …
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Drug discovery at the site of pulmonary tuberculosis
… and related to efficacy measurements using colony forming unit counts. Promising combinations of novel drugs were identified in a two dimensional synergy assay; these combinations showed synergistic activity in the infected macrophage model. The compounds showed marked differences in their …
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Evaluating microbial safety, postharvest quality, and consumer acceptability of fresh produce engraved with QR codes using a CO₂ laser labeling technology
… (E. coli) at the initial concentration of log 6 colony forming unit (CFU)/mL to the labeled fruit. The results showed that the population of rifampicin-resistant E. coli in QR-code labeled apples was statistically similar to the control. The third research chapter assessed consumers' perception …
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Growth Characteristics of Bifidobacteria in Infant Formulas
… by incubation at 37°C for 24 h. Mean maximum colony forming unit per milliliter (cfu/ml) for B. bifidum were 6.lxl06 (SB), 1.4xl0<sup>9</sup> (MB), l.lxl0<sup>7</sup> (CH), and l.8xl0<sup>9</sup> (C). Generation time (G) ranged from 96 (MB) to 322 (SB) min. Mean maximum bacterial counts for B. …
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Investigation of Silver Enhanced Dental Restorative Materials by the Development of an In Vitro Oral Biofilm Model
… formation, however it is considered a way of performing a reproducible experiment under controlled conditions. Obviously there are ethical limitations with in vivo studies in relation to caries and periodontal diseases. Therefore, different in vitro techniques have been developed and are …
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Evaluation of the Interplay between β-Lactamase Expression and Porin Loss in β-Lactam Susceptibility Profiles in Escherichia coli
Escherichia coli, a leading cause of both community- and hospital-acquired infections, has shown a marked increase in antimicrobial resistance driven by the acquisition of Extended-Spectrum β-Lactamases (ESBLs) and carbapenemases. However, β-lactam resistance in E. coli is often multifactorial, …
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A Novel, Codon-Based Approach to Preserve On-Tumor Activity and Reduce Off-Tumor Toxicity of Chimeric Antigen Receptor (CAR) T Cells
… vitro</em>. Furthermore, <em>in vitro</em> colony-forming unit assays demonstrated improved hematopoietic cell potential with S30 and R27 Hu8F4-CAR compared to wildtype Hu8F4-CAR. Lastly, CAR-T cells expressing the R27 Hu8F4-CAR variant showed reduced activity against healthy human HLA-A2+ …
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Accelerated Sepsis Diagnosis by Seamless Integration of Nucleic Acid Purification and Detection
… in very low concentrations, e.g., less than 1 colony-forming unit/ml. As a result, among the current sepsis diagnostic methods there is an unsatisfactory trade-off between the assay time and the specificity of the derived diagnostic information. Although the present qPCR-based test is more …
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New approaches to improve the cord blood unit hematopoietic progenitor cell content
… day, over 400 000 unrelated donor cord blood units have been stored in cord blood banks worldwide. To enable successful cord blood transplantation, recent efforts have been focused on finding ways to increase the hematopoietic progenitor cell content of cord blood units. In this study, factors …
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Novel Inhibitors for Isocitrate Lyase as a Potent Antitubercular Agent for Mycobacterium Tuberculosis
… assay and a significant impact in the colony-forming unit assay. The role of fatty acid metabolism in drug tolerance was investigated by testing three central carbon metabolism mutant strains against isoniazid. Discussion: From the three CL-54 analogues tested, only CL-54-04 caused a …
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