Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 116 for “"colocalization"”.
-
Colocalization analysis for multimodal optical microscopy
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-08-01
-
Colocalization between Akirin, the NuRD Complex, and Histone Modifications
… <p>Our study aimed to examine the colocalization of Akirin with the CHD family chromatin remodeling complex known as the nucleosome remodeling and deacetylase (NuRD) complex. <em>Drosophila</em> polytene chromosomes were used to visually identify colocalization between Akirin and …
-
Analyzing GFP-tagged cytoskeletal protein colocalization in human carcinoma cells
… we chose to characterize proteins based on the colocalization parameter-the amount of overlap between two objects or signals. We aimed to create a single parameter that quantitatively defined colocalization yet complemented biological intuition about a complicated system. Cell culture techniques …
-
Functional Connection Between Yeast Dynamin and Retromer at the Endosome
… between the Retromer and Vps1. My data show colocalization between Vps1 and the Retromer, and that Vps1 knockout cells show a decrease in Retromer targeting to endosomes, a phenotype reminiscent of human Alzheimer's disease. In order to evaluate the functional relationship of the Retromer and …
-
Phosphorylation kinetics of cardiac gap junction regulation during stress
… exhibit significantly decreased Cx43/N-cadherin colocalization during ischemia, while phospho-null mutant hearts retain Cx43/N-cadherin colocalization. Triton X-100 solubility assay indicates S365A/S373A mice have increased junctional Cx43 during ischemia. Additionally, we show that pS368 decay …
-
NEUROBIOLOGIA DEL SUICIDIO NEL DISTURBO DEPRESSIVO MAGGIORE: STUDIO MORFOLOGICO POST-MORTEM PER L¿INDAGINE DEL NETWORK GLIALE E VASCOLARE, CON ANALISI TOSSICOLOGICHE PER LA VALUTAZIONE DELL¿ADERENZA ALLA TERAPIA PSICHIATRICA
… cortex (dlPFC) and white matter. GFAP/S100β colocalization patterns varied by sex and brain region, with higher expression and overlap in male suicide cases. Conversely, densitometric analysis revealed higher expression levels in females, with significant regional differences. No significant …
-
Survival Of Mycobacterium Avium Subspecies Paratuberculosis In The Pol
… with cy-5 conjugated secondary antibodies, and colocalization of bacteria with each marker was evaluated separately using confocal scanning laser microscopy (cslm). In both tissue models, colocalization of viable map and m. Tuberculosis with the early endosomal marker, transferrin receptor …
-
De-Risking PET Radioligand Discovery for Neurodegenerative Diseases
… amyotrophic lateral sclerosis (ALS) tissues. Colocalization of specific binding with aggregate TDP-43 immunostaining was evaluated for isotopologues of 6 existing tau PET radioligands in varying structural classes. Three radioligands, [3H]MK-6240, [3H]GTP-1, and [3H]JNJ-067, showed no binding …
-
Enduring Effects of Early Life Inflammation on Stress-related Microglial, Neuronal and Behavioral phenotypes
… to mHFD or nLPS decreased neuronal densities, GR colocalization and increased microglial densities, activation and toll-like receptor 4 colocalization in the vHPC and amygdala subregions at adulthood, with similar findings in mHFD+nLPS females. Surprisingly, adult mHFD+nLPS males displayed …
-
Mechanisms Regulating Resistance Training Mediated Hypertrophy in Human Skeletal Muscle
… cell periphery and capillaries and concomitant colocalization with the lysosome suggesting an increased efficiency to sequester dietary amino acids and activate MyoPS in the trained state. Males had greater post-exercise mTOR colocalization with the lysosome, which may in part, explain the …
-
Single quantum dot tracking for quantitative molecular imaging
… to label a target protein and developing a new colocalization-based metric to quantify the degree of protein target labeling. We present evidence of protein target labeling by using this single-trajectory level colocalization metric in combination with nanoparticle mobility measurements. The …
-
Examining Cellular Interactions and Response to Chemotherapy in The Glioblastoma Perivascular Niche
… Day 8 to 14. These trends correlated with cell colocalization, indicating a chemotactic role for CXCL12 in enabling the migration of LN229 cells towards HUVECs in co-cultures. Von Willebrand factor (vWF) was co-expressed with glial fibrillary acidic protein (GFAP) in up to 40% of LN229 cells …
-
Exploring the Mitigation of TDP-43 Toxicity by Sis1 in Yeast
… with fluorescent proteins and examined for colocalization under fluorescent microscopy. However, while TDP-43 does appear to titrate Sis1 out of the nucleus, there was no evidence that the two proteins colocalized.
-
De novo designed protein switches to enable increased specificity of targeted therapeutics
… Using computational protein design, we developed colocalization- dependent switches which activate only on the surface of cells expressing a precise combination of antigens (Lajoie et al., 2020). We have shown that these designed protein switches are highly effective at discerning between closely …
-
The interaction of ELKS with Ca2+ channel β subunits may contribute to active zone organization
… fluorescent immunohistochemistry, we observed colocalization between ELKS1b and VDCC β4 at synapses in the molecular layer of the cerebellum, suggesting a synaptic role for the interaction of these two proteins. Analysis of a P/Q-type VDCC knockout mouse (Cacna1a-/-) revealed that the …
-
The kinetics of endosome processing
… by determining co-localization with HRP. Colocalization of the two markers in the endosome is studied by using the ability of HRP to catalyse the crosslinking of membrane marker in endosomes with DAB (3,3'-diaminobenzidine), rendering the membrane marker detergent insoluble. To study the …
-
A Cell Biological Determination of Integrator Subunit Localization
… with unique subcellular localizations.</strong> Colocalization studies with endogenous IntS3 and Cajal body marker, coilin, showed that these two proteins overlap, and from this we concluded that IntS3 localized to Cajal bodies. Additionally, colocalization studies with mCherry-tagged IntS2 and 7 …
-
Deconstructing G Protein-Coupled Receptor Dimer Pharmacology: Case Studies in Dopamine D1 and D2 Receptors
… In parallel, we also examined D1 and D2 receptor colocalization in striatal brain slices as well as in cultured medium spiny neurons. Although we found evidence using bacterial artificial chromosome-D1 and D2 reporter mice that D1 and D2 receptors are co-expressed in a small number of brain …
-
Cationic amphiphilic drug-induced autophagosome accumulation is due to autophagosome sequestration within vimentin intermediate filament networks resulting in prolonged autophagosome half-life
… degradation of long-lived proteins, and</p> <p>colocalization of GFP-LC3 and LAMP1 indicated an increase in</p> <p>autophagosome synthesis without compensatory increase in clearance.</p> <p>Autophagosome synthesis was blocked using 3-MA to monitor pre-existing</p> <p>autophagosome degradation. …
-
Sphingomyelinases and Lysosomal Trafficking in Ebolavirus Entry
… protein 40 (VP40-GFP) in 293FT cells. EBOVLP colocalization with lysosomal marker, Lamp1, was visualized using an immunofluorescence assay. Interestingly, colocalization occurred in non-permeabilized cells, suggesting an interaction with EBOVLPs and lysosomes at the cell surface. By applying …
Page 1 of 6