Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"colocalisation"”.
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Investigating the relationship between lung cancer and the infection with the parasite Toxoplasma gondii
… protocol to colocalise and measure the degree of colocalisation of Arg-1/T. gondii and iNOS/T. gondii parasite in 51 lung cancer samples. IHC results revealed expression of Arg-1, iNOS and T. gondii in the bronchiolar epithelium, smooth muscle cells of blood vessels, alveolar macrophages and type …
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Organelle remodeling and V-ATPase dynamics in the lysosome regeneration cycle
… was an increase in apparent recruitment (by colocalisation with the late endosomal/lysosomal marker lgp120/LAMP1) of the V1G1 subunit of V-ATPase to the lysosomal membrane during ELF (sucrosomes formation). However, colocalisation decreased after 4 hours of invertase-triggered reformation of …
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Optical imaging methods for the study of disease models from the nano to the mesoscale
… A software package was developed to analyse the colocalisation of a-syn with internalised vesicles, and the clustering of a-syn under differing synaptic calcium levels. The colocalisation of a-syn and internalised vesicles was found to be temperature independent, suggesting that a-syn is involved …
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Primary sclerosing cholangitis: from genetic risk to disease biology
… are expression quantitative trait loci (eQTLs). Colocalisation assesses the evidence that a single shared causal variant is responsible for driving PSC risk and gene expression via an eQTL. In order to assign dysregulated genes to PSC risk loci, I perform colocalisation with eQTLs mapped in …
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Genetic Determinants of Human Susceptibility to Infections: A Multi-faceted Exome-based Analysis in the UK Biobank
… statistical approaches, such as fine-mapping and colocalisation analyses, in refining GWAS findings and uncovering causal variants. The review also delves into the emerging field of pharmacogenomics, setting the stage for investigating genetic influences on drug responses, particularly in the …
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Neurochemical and functional characterisation of the Melanin-concentrating hormone system in the rat brain
… neurons in the rat hypothalamus for colocalisation with neuropeptides of interest. Therefore I performed an immunohistochemical colocalisation study using a specific antibody against MCHR-1 in combination with antibodies against hypothalamic neuropeptides. I showed that MCHR-1 …
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The effect of non-coding variants on gene transcription in human blood cell types
… association studies (GWAS) by means of colocalisation. I performed a colocalisation analysis and as an example I could show how a GWAS variant mechanistically exerts its effect on plateletcrit - a blood cell index studied in a recent GWAS (Astle et al., 2016). Finally, I drew a link …
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Common 'Inborn Errors' of Metabolism in the General Population
… IEM. At 24 of these 54 loci, genetic colocalisation detected shared genetic signals for IEM-related metabolites and phenotypes. For example, in line with norepinephrine deficiency causing dizziness on standing in severe cases of rare orthostatic hypotension (OMIM #223360), I identified …
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Primary biliary cholangitis: genetic risk, phenotypic stratification and disease pathogenesis
… power across GWA studies and present statistical colocalisation, a Bayesian approach that probabilistically determines whether a given locus is associated with two or more traits; whether a single, pleiotropic variant accounts for the association with each trait; and which variant across the locus …
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Investigating the Role of Inflammatory Biomarkers and Incretins in the Aetiology of Type 2 Diabetes and Coronary Heart Disease using Human Genetics
… of GIPR mono agonism. Bayesian multi-trait colocalisation was used to distinguish trait clusters driven by shared causal variants to identify independent causal variants driving specific trait associations. Findings: IL-6 levels (both measured and genetically predicted) were associated with …
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Apolipoprotein E and propagation of pathological tau in Alzheimer’s Disease
… tau and tau-containing presynapses. Greater colocalisation was observed in AD brain and in APOE4 carriers. Thus, in AD, presynapses and pathological tau appeared more prone to astrocytic internalisation, possibly influencing synapse loss and tau spread. APOE4 mainly increased colocalisation …
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Genetic architecture of transcript splicing in blood and phenotypic consequences
… and their genetic associations, I perform colocalisation analysis of these with the generated spliceQTLs to explore their shared genetic architecture. This reveals that many splice events and molecular phenotypes appear to be regulated by shared genetic effects, and through examples …
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The role of Poly (ADP-ribose) polymerase in the regulation of innate recognition of immunological receptors expressed on human macrophages.
… to LPS. It was found that PJ-34 reduced the colocalisation of these pair of receptors and regulates their expression at gene level. Furthermore, PJ-34 regulates expression of a number of proteins on the THP-1 cells in response to LPS.
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An investigation into Toxoplasma gondii infection and the expression of Arginase-1 and iNOS in human lung tissue
… could be seen between T. gondii and iNOS. Colocalisation analysis, using grid coordinates overlayed on the matched images, found a significant positive association in regions with high Arginase-1 and high T. gondii staining. Also, a negative association between iNOS and T. gondii high …
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Quantified phenotype analysis in a cell model for Autosomal Dominant Retinitis Pigmentosa
… had a phenotype more similar to wildtype. Colocalisation was assessed using a simple approach of overlapping volume. As co-expression of rhodopsin and calnexin during time-lapse acquisition induced cytotoxicity and accelerated cellular death, we assessed phototoxicity caused by blue light …
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Shedding Light on the Biological Effects of Ionising Radiation on DNA Using Advanced Optical Microscopy
… foci by a factor of 2-5 and overestimated their colocalisation. Therefore, superresolution is not only useful in investigating foci structure, but will provide significant improvements in identifying the spatial distribution of correlated damage sites along high-LET tracks.
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Developing a novel Drosophila model to study Menin biological function
… from the cellular blastoderm stage onwards. Colocalisation with HDAC1 was observed in both contexts, consistent with the conserved mammalian Menin-HDAC1 association. Biochemical characterisation demonstrated that HA-tagged Menin can be immunoprecipitated from dissected larval brain and adult …
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Human GINS, a conserved DNA replication factor and candidate cancer marker
… to that seen for the MCMs. However, confusingly, colocalisation between GINS and MCMs and between the GINS subunits themselves is poor. Investigations are continuing to understand this conundrum. Given the value of MCM proteins as specific and sensitive markers for cancer screening, I investigated …
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Discovery of the novel mouFSnrp gene and the characterisation of its in situ expression profile during mouse neurogenesis
… in situ hybridisation and immunohistochemical colocalisation studies, interesting insights into the lineage and ontogeny of mouFSnrp expression during brain development were revealed. These results indicate that mouFSnrp expression originates in neural stem cells of the developing cortex, and …
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Étude du clivage de l'hémagglutinine de l'influenza par la matriptase
… La microscopie confocale a également montré la colocalisation du virus de l’influenza avec la matriptase dans les endosomes lors de l’entrée du virus dans la cellule. L'utilisation de la technique d'ARN interférents contre la matriptase dans un modèle cellulaire a permis de réduire la …
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