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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 87 for “"collagenase"”.
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Experimental Models of Brain Hemorrhage Using Clostridial Collagenase
<p>Stroke is the third leading cause of death and is the leading cause of disability in the United States, and the most severe form is known as intracerebral hemorrhage, which is bleeding into the brain. The goal of this study is to establish novel animal models to buildup foundations for …
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Development of Collagen Mimetic Mini-Fibrils with Tunable Collagenase Susceptibility
… the interactions of MMP1 and the bacterial collagenase ColG with native type I collagen. Kinetic assays of the enzyme reactions revealed that the triple helix was the most susceptible conformation of collagen to MMP1, while the degradation of the fibrillar form or the unfolded form of …
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Regulation der Metalloproteinasen Stromelysin und Collagenase durch Interferon in retinalen Pigmentepithelzellen
… ARPE-19 die Metalloproteinasen Stromelysin und Collagenase produziert werden und ob die Genexpression dieser Metalloproteinasen durch eine Behandlung der Zellen mit humanen Interferonen stimuliert werden kann. Der Nachweis sollte auf der mRNA-Ebene erfolgen. Als Nachweismethoden dienten die …
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Studies on the regulation of extracellular collagenase production by vibrio alginolyticus
Vibrio alginolyticus synthesized extracellular collagenase in a highly aerated peptone medium at the late-exponential and early-stationary phases of growth. Collagenase synthesis was subject to end-product repression and was repressed by various amino acids and ammonium ions. Glutamine caused …
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Characterization and regulation of serine exoproteases and collagenase in vibrio alginolyticus
The production of an extracellular collagenase and serine proteases by Vibrio alginolyticus during stationary phase was inhibited by a temperature shift from 30 to 37°C and by lack of oxygen. V. alginolyticus had identical growth rates at 30 and 37°C. Aeration did not affect the growth rate of …
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A Method to Improve Cartilage Integration
… graft. This method used a combination of collagenase treatment (as a means of degrading the ECM within the ZCD) and chondrocyte chemotaxis (as a means of improving chondrocyte migration into the ZCD and to the cartilage periphery). Results indicate that treating bovine articular cartilage …
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The mechanism of collagenolysis : a substrate-centric view
… suggests that the enzymes that degrade collagen, collagenases, physically unwind the triple-helical structure of collagen to gain access to the peptide bond that is cleaved. This unwinding mechanism would in principle have large energetic requirements, but neither ATP nor other energy-rich …
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Regulation of nitrogen metabolism in vibrio alginolyticus
… by temperature and oxygen has been reported for collagenase and prntease production by V. alginolyticus (Hare et al., 1981). V. alginolyticus had identical growth rates at 30 and 37°C. The histidine-utilization (hut) enzymes were coordinately induced by histidine. The inducible nitrogen catabolic …
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Improving the delivery and efficacy of molecular medicine via extracellular matrix modulation : insights from intravital microscopy
… collagen matrix directly with purified bacterial collagenase, or indirectly with relaxin treatment, resulted in increased diffusion coefficients of macromolecules within tumors in vivo. In order to quantitate the changes in collagen content and structure induced by relaxin treatment, we adapted …
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Molecular biology studies on the extracellular serine proteases of Vibrio alginolyticus
… several extracellular serine proteases and a collagenase during the stationary growth phase. The aim of this study was to investigate alkaline serine protease production by this organism, and to attempt the cloning and expression of a V.alginolyticus protease gene in Escherichia coli.
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Analysis of the Proteoglycan Content in Fresh and Cryopreserved Porcine Cardiovascular Tissues
… by sequential digestion of the tissues with collagenase, elastase, and papain. Glycosaminoglycans (GAGs) of the PGs were isolated and quantitated. Gdn-HCl extracted about 61% and 62% of the total GAG (proteoglycan) material from cryopreserved and fresh tissues, respectively. Collagenase …
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The Isolation and Differentiation of Adipose Derived Mesenchymal Stem Cells
… a non-enzymatic sonication based method to the collagenase digestion based method of isolating MSCs from human adipose. Sonication yielded fewer total cells than collagenase. However, alizarin red staining revealed that cells obtained using sonication possessed a stronger osteogenic capacity …
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The role of substance P in experimental intracerebral haemorrhage.
… rats were used, and variously subjected to collagenase ICH, autologous ICH, intracerebral thrombin injection and intracerebral injection of SP. The sequelae of these various injuries was assessed, as well as the effect of antagonists to the main substance P receptor (NK1R), using functional …
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Equine superficial digital flexor tendon evaluation using low field magnetic resonance imaging and ultrasonography
… at 3, 4, 6, 8, and 16 week time points post collagenase injection. MR signal intensities (T1-weighted, T2-weighted, and STIR sequences) and tendon histology (collagen content, proteoglycan content, and collagen fiber alignment) was performed at the same time points. At four weeks following …
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The role of unfolded states in collagen degradation
… helix does not fit into the active site of collagenases, the enzymes responsible of cleaving collagen. Moreover, the scissile bond that is degraded during collagenolysis is hidden from solvent. Therefore it is widely agreed that collagen unfolding must occur in order for collagenolysis to …
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A Lorentz-force actuated controllable needle-free drug delivery system
… testing on murine tissue using a bacterial collagenase.
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In vitro endothelialization of vascular prostheses: a possible means to improve the early patency of small diameter vascular grafts
… veins were excised and cannulated before 0.1 % collagenase (CLS II) was instillated for 15 minutes to detach EC's. During the following growth under tissue culture conditions, we could demonstrate that a short transport time between vein excision and collagenase application is essential for a …
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In vitro characterization and in vivo assessment of equine tendon-derived progenitor cells
… stem cells. Cell suspensions obtained via collagenase digestion of equine lateral digital extensor tendon (n=4) were serially transferred into adherent plates every 12 hrs for 4 days. TDPCs obtained from the initial, third and seventh preplates were used for subsequent analyses. …
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